Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3272 and HP15_3273 are separated by 407 nucleotides HP15_3273 and HP15_3274 are separated by 90 nucleotides HP15_3274 and HP15_3275 are separated by 66 nucleotides
HP15_3272: HP15_3272 - inositol-1(or 4)-monophosphatase, at 3,430,905 to 3,431,729
_3272
HP15_3273: HP15_3273 - nucleoid occlusion protein, at 3,432,137 to 3,432,724
_3273
HP15_3274: HP15_3274 - thiazole synthase, at 3,432,815 to 3,433,624
_3274
HP15_3275: HP15_3275 - ThiS, thiamine-biosynthesis, at 3,433,691 to 3,433,891
_3275
Position (kb)
3432
3433 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3431.331 kb on - strand, within HP15_3272 at 3431.482 kb on + strand, within HP15_3272 at 3431.501 kb on + strand, within HP15_3272 at 3431.556 kb on - strand, within HP15_3272 at 3431.695 kb on + strand at 3431.703 kb on - strand at 3431.720 kb on - strand at 3431.736 kb on - strand at 3431.792 kb on + strand at 3432.284 kb on + strand, within HP15_3273 at 3432.321 kb on - strand, within HP15_3273 at 3432.361 kb on - strand, within HP15_3273 at 3432.414 kb on + strand, within HP15_3273 at 3432.424 kb on + strand, within HP15_3273 at 3432.424 kb on + strand, within HP15_3273 at 3432.424 kb on + strand, within HP15_3273 at 3432.432 kb on - strand, within HP15_3273 at 3432.513 kb on - strand, within HP15_3273 at 3432.533 kb on - strand, within HP15_3273 at 3432.553 kb on - strand, within HP15_3273 at 3432.604 kb on + strand, within HP15_3273 at 3432.755 kb on + strand at 3432.784 kb on + strand at 3432.784 kb on + strand at 3433.620 kb on + strand at 3433.628 kb on - strand at 3433.628 kb on - strand at 3433.661 kb on - strand at 3433.683 kb on - strand at 3433.687 kb on + strand at 3433.689 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 3,431,331 - HP15_3272 0.52 +2.3 3,431,482 + HP15_3272 0.70 +0.7 3,431,501 + HP15_3272 0.72 -1.0 3,431,556 - HP15_3272 0.79 +1.2 3,431,695 + +2.5 3,431,703 - +1.5 3,431,720 - +0.4 3,431,736 - -1.7 3,431,792 + +1.6 3,432,284 + HP15_3273 0.25 +0.0 3,432,321 - HP15_3273 0.31 +2.1 3,432,361 - HP15_3273 0.38 -1.3 3,432,414 + HP15_3273 0.47 -1.9 3,432,424 + HP15_3273 0.49 +1.9 3,432,424 + HP15_3273 0.49 +2.3 3,432,424 + HP15_3273 0.49 +1.6 3,432,432 - HP15_3273 0.50 +0.0 3,432,513 - HP15_3273 0.64 -0.1 3,432,533 - HP15_3273 0.67 +2.3 3,432,553 - HP15_3273 0.71 +1.5 3,432,604 + HP15_3273 0.79 -0.1 3,432,755 + -0.1 3,432,784 + -0.6 3,432,784 + +0.5 3,433,620 + -0.4 3,433,628 - +0.1 3,433,628 - +1.2 3,433,661 - +1.7 3,433,683 - +0.3 3,433,687 + +0.0 3,433,689 - -0.2
Or see this region's nucleotide sequence