Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3030 and HP15_3031 are separated by 100 nucleotides HP15_3031 and HP15_3032 are separated by 12 nucleotides HP15_3032 and HP15_3033 are separated by 3 nucleotides HP15_3033 and HP15_3034 are separated by 159 nucleotides
HP15_3030: HP15_3030 - histidine/lysine/arginine/ornithine transporter subunit, at 3,174,688 to 3,175,458
_3030
HP15_3031: HP15_3031 - L-arginine ABC transporter, periplasmic substrate-binding component ArtJ/HisJ/ArtI/AotJ/ArgT <small>(from data)</small>, at 3,175,559 to 3,176,311
_3031
HP15_3032: HP15_3032 - amino acid ABC transporter, inner membrane subunit, at 3,176,324 to 3,177,067
_3032
HP15_3033: HP15_3033 - amino acid ABC transporter, inner membrane subunit, at 3,177,071 to 3,177,793
_3033
HP15_3034: HP15_3034 - succinylglutamate desuccinylase, at 3,177,953 to 3,179,008
_3034
Position (kb) 3176
3177
3178 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3175.368 kb on + strand, within HP15_3030 at 3175.427 kb on + strand at 3175.440 kb on - strand at 3175.465 kb on + strand at 3175.478 kb on - strand at 3175.478 kb on - strand at 3175.717 kb on + strand, within HP15_3031 at 3175.784 kb on + strand, within HP15_3031 at 3175.959 kb on - strand, within HP15_3031 at 3175.993 kb on - strand, within HP15_3031 at 3176.057 kb on + strand, within HP15_3031 at 3176.066 kb on + strand, within HP15_3031 at 3176.161 kb on + strand, within HP15_3031 at 3176.166 kb on + strand, within HP15_3031 at 3176.189 kb on + strand, within HP15_3031 at 3176.213 kb on - strand, within HP15_3031 at 3176.213 kb on - strand, within HP15_3031 at 3176.269 kb on - strand at 3176.454 kb on + strand, within HP15_3032 at 3176.462 kb on - strand, within HP15_3032 at 3176.462 kb on - strand, within HP15_3032 at 3176.518 kb on + strand, within HP15_3032 at 3176.526 kb on - strand, within HP15_3032 at 3176.533 kb on + strand, within HP15_3032 at 3176.544 kb on + strand, within HP15_3032 at 3176.752 kb on + strand, within HP15_3032 at 3176.760 kb on - strand, within HP15_3032 at 3176.770 kb on - strand, within HP15_3032 at 3176.770 kb on - strand, within HP15_3032 at 3176.776 kb on - strand, within HP15_3032 at 3176.839 kb on - strand, within HP15_3032 at 3176.952 kb on - strand, within HP15_3032 at 3177.053 kb on + strand at 3177.053 kb on + strand at 3177.144 kb on - strand, within HP15_3033 at 3177.159 kb on + strand, within HP15_3033 at 3177.239 kb on + strand, within HP15_3033 at 3177.262 kb on + strand, within HP15_3033 at 3177.270 kb on - strand, within HP15_3033 at 3177.304 kb on + strand, within HP15_3033 at 3177.535 kb on + strand, within HP15_3033 at 3177.565 kb on - strand, within HP15_3033 at 3177.565 kb on - strand, within HP15_3033 at 3177.570 kb on - strand, within HP15_3033 at 3177.629 kb on + strand, within HP15_3033 at 3177.720 kb on + strand, within HP15_3033 at 3177.971 kb on + strand at 3177.981 kb on + strand at 3178.004 kb on + strand at 3178.044 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 3,175,368 + HP15_3030 0.88 +3.1 3,175,427 + +1.2 3,175,440 - -0.9 3,175,465 + +0.0 3,175,478 - +1.3 3,175,478 - +1.8 3,175,717 + HP15_3031 0.21 +1.2 3,175,784 + HP15_3031 0.30 +0.4 3,175,959 - HP15_3031 0.53 -0.3 3,175,993 - HP15_3031 0.58 -0.6 3,176,057 + HP15_3031 0.66 -0.4 3,176,066 + HP15_3031 0.67 +2.7 3,176,161 + HP15_3031 0.80 +0.8 3,176,166 + HP15_3031 0.81 +0.3 3,176,189 + HP15_3031 0.84 +0.2 3,176,213 - HP15_3031 0.87 +0.1 3,176,213 - HP15_3031 0.87 +1.7 3,176,269 - -1.6 3,176,454 + HP15_3032 0.17 +0.2 3,176,462 - HP15_3032 0.19 +0.2 3,176,462 - HP15_3032 0.19 -1.0 3,176,518 + HP15_3032 0.26 +1.0 3,176,526 - HP15_3032 0.27 -0.9 3,176,533 + HP15_3032 0.28 +0.0 3,176,544 + HP15_3032 0.30 +1.3 3,176,752 + HP15_3032 0.58 +0.4 3,176,760 - HP15_3032 0.59 +1.0 3,176,770 - HP15_3032 0.60 +1.8 3,176,770 - HP15_3032 0.60 -0.3 3,176,776 - HP15_3032 0.61 -0.0 3,176,839 - HP15_3032 0.69 +0.5 3,176,952 - HP15_3032 0.84 +2.1 3,177,053 + +0.7 3,177,053 + +2.5 3,177,144 - HP15_3033 0.10 -1.2 3,177,159 + HP15_3033 0.12 +0.6 3,177,239 + HP15_3033 0.23 -1.7 3,177,262 + HP15_3033 0.26 +2.0 3,177,270 - HP15_3033 0.28 -0.5 3,177,304 + HP15_3033 0.32 +1.7 3,177,535 + HP15_3033 0.64 +1.0 3,177,565 - HP15_3033 0.68 -1.2 3,177,565 - HP15_3033 0.68 -0.5 3,177,570 - HP15_3033 0.69 +0.6 3,177,629 + HP15_3033 0.77 -0.2 3,177,720 + HP15_3033 0.90 +1.2 3,177,971 + +0.0 3,177,981 + -0.7 3,178,004 + -2.0 3,178,044 - -0.9
Or see this region's nucleotide sequence