Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2587 and HP15_2588 are separated by 41 nucleotides HP15_2588 and HP15_2589 are separated by 34 nucleotides HP15_2589 and HP15_2590 overlap by 4 nucleotides HP15_2590 and HP15_2591 overlap by 4 nucleotides HP15_2591 and HP15_2592 are separated by 9 nucleotides HP15_2592 and HP15_2593 overlap by 8 nucleotides
HP15_2587: HP15_2587 - monovalent cation/H+ antiporter subunit A, at 2,736,626 to 2,738,935
_2587
HP15_2588: HP15_2588 - Na+/H+ antiporter MnhB subunit-related protein, at 2,738,977 to 2,739,357
_2588
HP15_2589: HP15_2589 - NADH:ubiquinone/quinone oxidoreductase, chain 4L, at 2,739,392 to 2,739,739
_2589
HP15_2590: HP15_2590 - monovalent cation/H+ antiporter subunit D, at 2,739,736 to 2,741,250
_2590
HP15_2591: HP15_2591 - monovalent cation/H+ antiporter subunit E, at 2,741,247 to 2,741,720
_2591
HP15_2592: HP15_2592 - multiple resistance and pH regulation protein F, at 2,741,730 to 2,742,002
_2592
HP15_2593: HP15_2593 - monovalent cation/proton antiporter, MnhG/PhaG subunit, at 2,741,995 to 2,742,402
_2593
Position (kb)
2739
2740
2741
2742 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2738.744 kb on - strand at 2738.781 kb on + strand at 2738.786 kb on + strand at 2738.794 kb on - strand at 2738.847 kb on - strand at 2738.867 kb on - strand at 2738.915 kb on + strand at 2739.050 kb on + strand, within HP15_2588 at 2739.076 kb on - strand, within HP15_2588 at 2739.207 kb on - strand, within HP15_2588 at 2739.207 kb on - strand, within HP15_2588 at 2739.232 kb on + strand, within HP15_2588 at 2739.254 kb on + strand, within HP15_2588 at 2739.302 kb on - strand, within HP15_2588 at 2739.316 kb on + strand, within HP15_2588 at 2739.406 kb on + strand at 2739.422 kb on - strand at 2739.499 kb on - strand, within HP15_2589 at 2739.615 kb on + strand, within HP15_2589 at 2739.676 kb on - strand, within HP15_2589 at 2739.699 kb on + strand, within HP15_2589 at 2739.792 kb on - strand at 2739.921 kb on + strand, within HP15_2590 at 2739.942 kb on - strand, within HP15_2590 at 2739.944 kb on - strand, within HP15_2590 at 2739.946 kb on + strand, within HP15_2590 at 2739.980 kb on - strand, within HP15_2590 at 2740.028 kb on + strand, within HP15_2590 at 2740.262 kb on + strand, within HP15_2590 at 2740.280 kb on - strand, within HP15_2590 at 2740.318 kb on + strand, within HP15_2590 at 2740.328 kb on + strand, within HP15_2590 at 2740.338 kb on - strand, within HP15_2590 at 2740.346 kb on - strand, within HP15_2590 at 2740.484 kb on + strand, within HP15_2590 at 2740.484 kb on + strand, within HP15_2590 at 2740.499 kb on - strand, within HP15_2590 at 2740.529 kb on - strand, within HP15_2590 at 2740.561 kb on - strand, within HP15_2590 at 2740.563 kb on - strand, within HP15_2590 at 2740.563 kb on - strand, within HP15_2590 at 2740.563 kb on - strand, within HP15_2590 at 2740.671 kb on + strand, within HP15_2590 at 2740.793 kb on + strand, within HP15_2590 at 2740.807 kb on + strand, within HP15_2590 at 2740.911 kb on + strand, within HP15_2590 at 2741.000 kb on - strand, within HP15_2590 at 2741.211 kb on + strand at 2741.258 kb on + strand at 2741.593 kb on + strand, within HP15_2591 at 2741.666 kb on + strand, within HP15_2591 at 2741.674 kb on - strand at 2741.696 kb on - strand at 2741.804 kb on - strand, within HP15_2592 at 2741.809 kb on + strand, within HP15_2592 at 2741.833 kb on - strand, within HP15_2592 at 2741.898 kb on + strand, within HP15_2592 at 2742.151 kb on + strand, within HP15_2593 at 2742.159 kb on - strand, within HP15_2593
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 2,738,744 - -1.3 2,738,781 + +1.8 2,738,786 + +1.0 2,738,794 - +1.1 2,738,847 - +0.7 2,738,867 - -1.9 2,738,915 + -1.7 2,739,050 + HP15_2588 0.19 +1.0 2,739,076 - HP15_2588 0.26 +1.1 2,739,207 - HP15_2588 0.60 -0.6 2,739,207 - HP15_2588 0.60 +0.3 2,739,232 + HP15_2588 0.67 -0.1 2,739,254 + HP15_2588 0.73 +1.1 2,739,302 - HP15_2588 0.85 +0.2 2,739,316 + HP15_2588 0.89 +0.9 2,739,406 + -0.1 2,739,422 - -1.1 2,739,499 - HP15_2589 0.31 +0.1 2,739,615 + HP15_2589 0.64 +1.3 2,739,676 - HP15_2589 0.82 -0.3 2,739,699 + HP15_2589 0.88 -0.7 2,739,792 - -0.6 2,739,921 + HP15_2590 0.12 +0.2 2,739,942 - HP15_2590 0.14 +1.6 2,739,944 - HP15_2590 0.14 +1.5 2,739,946 + HP15_2590 0.14 -2.1 2,739,980 - HP15_2590 0.16 +1.4 2,740,028 + HP15_2590 0.19 +0.6 2,740,262 + HP15_2590 0.35 +0.4 2,740,280 - HP15_2590 0.36 +0.1 2,740,318 + HP15_2590 0.38 -2.7 2,740,328 + HP15_2590 0.39 +0.7 2,740,338 - HP15_2590 0.40 -0.7 2,740,346 - HP15_2590 0.40 -1.4 2,740,484 + HP15_2590 0.49 -1.0 2,740,484 + HP15_2590 0.49 -0.3 2,740,499 - HP15_2590 0.50 +4.0 2,740,529 - HP15_2590 0.52 +0.1 2,740,561 - HP15_2590 0.54 -0.8 2,740,563 - HP15_2590 0.55 -0.4 2,740,563 - HP15_2590 0.55 +1.5 2,740,563 - HP15_2590 0.55 -0.8 2,740,671 + HP15_2590 0.62 +2.4 2,740,793 + HP15_2590 0.70 +0.1 2,740,807 + HP15_2590 0.71 +0.8 2,740,911 + HP15_2590 0.78 -0.8 2,741,000 - HP15_2590 0.83 -0.3 2,741,211 + +0.3 2,741,258 + +1.2 2,741,593 + HP15_2591 0.73 -0.1 2,741,666 + HP15_2591 0.88 +0.2 2,741,674 - +1.9 2,741,696 - +1.5 2,741,804 - HP15_2592 0.27 -0.7 2,741,809 + HP15_2592 0.29 -1.0 2,741,833 - HP15_2592 0.38 -0.9 2,741,898 + HP15_2592 0.62 +0.2 2,742,151 + HP15_2593 0.38 +0.1 2,742,159 - HP15_2593 0.40 +1.1
Or see this region's nucleotide sequence