Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_248 and HP15_249 are separated by 16 nucleotides HP15_249 and HP15_250 are separated by 28 nucleotides HP15_250 and HP15_251 are separated by 68 nucleotides
HP15_248: HP15_248 - diaminopimelate epimerase, at 236,824 to 237,738
_248
HP15_249: HP15_249 - protein containing DUF484, at 237,755 to 238,501
_249
HP15_250: HP15_250 - site-specific tyrosine recombinase XerC, at 238,530 to 239,462
_250
HP15_251: HP15_251 - diguanylate cyclase, at 239,531 to 241,024
_251
Position (kb) 238
239
240 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 237.667 kb on + strand at 237.677 kb on - strand at 237.748 kb on - strand at 237.751 kb on + strand at 237.875 kb on - strand, within HP15_249 at 237.935 kb on - strand, within HP15_249 at 237.942 kb on + strand, within HP15_249 at 238.020 kb on - strand, within HP15_249 at 238.153 kb on + strand, within HP15_249 at 238.177 kb on - strand, within HP15_249 at 238.222 kb on + strand, within HP15_249 at 238.381 kb on + strand, within HP15_249 at 238.520 kb on - strand at 238.530 kb on - strand at 238.929 kb on - strand, within HP15_250 at 239.026 kb on - strand, within HP15_250 at 239.031 kb on - strand, within HP15_250 at 239.031 kb on - strand, within HP15_250 at 239.102 kb on + strand, within HP15_250 at 239.112 kb on - strand, within HP15_250 at 239.193 kb on + strand, within HP15_250 at 239.330 kb on - strand, within HP15_250 at 239.330 kb on - strand, within HP15_250 at 239.354 kb on + strand, within HP15_250 at 239.362 kb on - strand, within HP15_250 at 239.548 kb on - strand at 239.605 kb on - strand at 239.630 kb on - strand at 239.663 kb on + strand at 239.844 kb on + strand, within HP15_251 at 239.865 kb on - strand, within HP15_251 at 239.878 kb on + strand, within HP15_251 at 239.883 kb on + strand, within HP15_251 at 239.922 kb on - strand, within HP15_251 at 240.021 kb on + strand, within HP15_251 at 240.021 kb on + strand, within HP15_251 at 240.021 kb on + strand, within HP15_251 at 240.029 kb on - strand, within HP15_251 at 240.029 kb on - strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.104 kb on + strand, within HP15_251 at 240.112 kb on + strand, within HP15_251 at 240.112 kb on - strand, within HP15_251 at 240.112 kb on - strand, within HP15_251 at 240.125 kb on - strand, within HP15_251 at 240.135 kb on - strand, within HP15_251 at 240.206 kb on + strand, within HP15_251 at 240.214 kb on + strand, within HP15_251 at 240.233 kb on + strand, within HP15_251 at 240.236 kb on - strand, within HP15_251 at 240.236 kb on - strand, within HP15_251 at 240.246 kb on - strand, within HP15_251 at 240.263 kb on - strand, within HP15_251 at 240.327 kb on + strand, within HP15_251 at 240.329 kb on - strand, within HP15_251 at 240.357 kb on - strand, within HP15_251 at 240.436 kb on + strand, within HP15_251 at 240.444 kb on - strand, within HP15_251 at 240.444 kb on - strand, within HP15_251 at 240.451 kb on + strand, within HP15_251
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 237,667 + -0.6 237,677 - +1.5 237,748 - -0.7 237,751 + -0.2 237,875 - HP15_249 0.16 -0.8 237,935 - HP15_249 0.24 -0.7 237,942 + HP15_249 0.25 +1.5 238,020 - HP15_249 0.35 -1.6 238,153 + HP15_249 0.53 +2.7 238,177 - HP15_249 0.56 -0.5 238,222 + HP15_249 0.63 -1.2 238,381 + HP15_249 0.84 -0.7 238,520 - -2.0 238,530 - -0.8 238,929 - HP15_250 0.43 -0.7 239,026 - HP15_250 0.53 -1.1 239,031 - HP15_250 0.54 -0.9 239,031 - HP15_250 0.54 -0.5 239,102 + HP15_250 0.61 +2.4 239,112 - HP15_250 0.62 -0.2 239,193 + HP15_250 0.71 +1.0 239,330 - HP15_250 0.86 +1.8 239,330 - HP15_250 0.86 +1.0 239,354 + HP15_250 0.88 -0.3 239,362 - HP15_250 0.89 +0.2 239,548 - +1.0 239,605 - -0.6 239,630 - -0.4 239,663 + +0.2 239,844 + HP15_251 0.21 +0.0 239,865 - HP15_251 0.22 -0.7 239,878 + HP15_251 0.23 -1.2 239,883 + HP15_251 0.24 +0.2 239,922 - HP15_251 0.26 -0.4 240,021 + HP15_251 0.33 -0.2 240,021 + HP15_251 0.33 +1.6 240,021 + HP15_251 0.33 +2.1 240,029 - HP15_251 0.33 -0.5 240,029 - HP15_251 0.33 -1.3 240,104 + HP15_251 0.38 +2.8 240,104 + HP15_251 0.38 +0.6 240,104 + HP15_251 0.38 -1.0 240,104 + HP15_251 0.38 +1.8 240,104 + HP15_251 0.38 +0.2 240,112 + HP15_251 0.39 +3.8 240,112 - HP15_251 0.39 +1.3 240,112 - HP15_251 0.39 +2.3 240,125 - HP15_251 0.40 +1.6 240,135 - HP15_251 0.40 -0.4 240,206 + HP15_251 0.45 -0.2 240,214 + HP15_251 0.46 -0.3 240,233 + HP15_251 0.47 +0.9 240,236 - HP15_251 0.47 +2.4 240,236 - HP15_251 0.47 -0.8 240,246 - HP15_251 0.48 -1.2 240,263 - HP15_251 0.49 -1.0 240,327 + HP15_251 0.53 +2.2 240,329 - HP15_251 0.53 +1.2 240,357 - HP15_251 0.55 -0.3 240,436 + HP15_251 0.61 -0.1 240,444 - HP15_251 0.61 +1.8 240,444 - HP15_251 0.61 -0.6 240,451 + HP15_251 0.62 +1.8
Or see this region's nucleotide sequence