Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2201 and HP15_2202 overlap by 9 nucleotides HP15_2202 and HP15_2203 are separated by 256 nucleotides HP15_2203 and HP15_2204 are separated by 90 nucleotides HP15_2204 and HP15_2205 are separated by 130 nucleotides
HP15_2201: HP15_2201 - transcriptional regulator, LysR family, at 2,318,616 to 2,319,499
_2201
HP15_2202: HP15_2202 - tRNA-(ms(2)io(6)a)-hydroxylase, at 2,319,491 to 2,320,099
_2202
HP15_2203: HP15_2203 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 2,320,356 to 2,322,950
_2203
HP15_2204: HP15_2204 - membrane protein containing DUF81, at 2,323,041 to 2,323,787
_2204
HP15_2205: HP15_2205 - sodium/solute symporter, at 2,323,918 to 2,325,363
_2205
Position (kb)
2320
2321
2322
2323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2319.479 kb on - strand at 2319.616 kb on + strand, within HP15_2202 at 2319.672 kb on + strand, within HP15_2202 at 2319.688 kb on - strand, within HP15_2202 at 2319.715 kb on + strand, within HP15_2202 at 2319.752 kb on - strand, within HP15_2202 at 2319.882 kb on - strand, within HP15_2202 at 2319.904 kb on + strand, within HP15_2202 at 2319.912 kb on + strand, within HP15_2202 at 2319.925 kb on + strand, within HP15_2202 at 2319.995 kb on - strand, within HP15_2202 at 2320.028 kb on + strand, within HP15_2202 at 2320.038 kb on + strand, within HP15_2202 at 2320.045 kb on + strand at 2320.061 kb on - strand at 2320.154 kb on + strand at 2323.027 kb on + strand at 2323.087 kb on - strand at 2323.094 kb on - strand at 2323.174 kb on - strand, within HP15_2204 at 2323.536 kb on + strand, within HP15_2204 at 2323.613 kb on - strand, within HP15_2204 at 2323.613 kb on - strand, within HP15_2204 at 2323.791 kb on + strand at 2323.879 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 2,319,479 - -2.4 2,319,616 + HP15_2202 0.21 +0.8 2,319,672 + HP15_2202 0.30 -0.7 2,319,688 - HP15_2202 0.32 -0.4 2,319,715 + HP15_2202 0.37 -0.2 2,319,752 - HP15_2202 0.43 +1.1 2,319,882 - HP15_2202 0.64 -2.5 2,319,904 + HP15_2202 0.68 -0.4 2,319,912 + HP15_2202 0.69 -1.9 2,319,925 + HP15_2202 0.71 -0.6 2,319,995 - HP15_2202 0.83 -0.8 2,320,028 + HP15_2202 0.88 -1.2 2,320,038 + HP15_2202 0.90 -0.3 2,320,045 + -1.1 2,320,061 - -1.0 2,320,154 + +1.4 2,323,027 + -1.0 2,323,087 - +0.3 2,323,094 - +0.3 2,323,174 - HP15_2204 0.18 +1.2 2,323,536 + HP15_2204 0.66 -0.5 2,323,613 - HP15_2204 0.77 +0.2 2,323,613 - HP15_2204 0.77 +2.2 2,323,791 + +0.2 2,323,879 + -0.2
Or see this region's nucleotide sequence