Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt orn and tRNA-Gly-4 are separated by 200 nucleotides tRNA-Gly-4 and tRNA-Gly-3 are separated by 124 nucleotides tRNA-Gly-3 and tRNA-Gly-2 are separated by 122 nucleotides tRNA-Gly-2 and tRNA-Gly-1 are separated by 126 nucleotides tRNA-Gly-1 and SO0595 are separated by 436 nucleotides
SO0592: orn - oligoribonuclease (NCBI ptt file), at 619,424 to 619,969
orn
SO_t009: tRNA-Gly-4 - tRNA-Gly (RefSeq), at 620,170 to 620,245
tRNA-Gly-4
SO_t010: tRNA-Gly-3 - tRNA-Gly (RefSeq), at 620,370 to 620,445
tRNA-Gly-3
SO_t011: tRNA-Gly-2 - tRNA-Gly (RefSeq), at 620,568 to 620,643
tRNA-Gly-2
SO_t012: tRNA-Gly-1 - tRNA-Gly (RefSeq), at 620,770 to 620,845
tRNA-Gly-1
SO0595: SO0595 - hypothetical protein (NCBI ptt file), at 621,282 to 621,833
SO0595
Position (kb)
620
621 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 620.313 kb on + strand at 620.606 kb on + strand, within tRNA-Gly-2 at 620.750 kb on + strand at 620.750 kb on + strand at 620.808 kb on + strand, within tRNA-Gly-1 at 620.936 kb on - strand at 620.950 kb on - strand at 620.951 kb on - strand at 620.953 kb on + strand at 620.953 kb on + strand at 620.953 kb on + strand at 620.953 kb on + strand at 620.953 kb on + strand at 620.958 kb on + strand at 620.958 kb on + strand at 620.961 kb on - strand at 620.961 kb on - strand at 620.961 kb on - strand at 620.966 kb on - strand at 620.966 kb on - strand at 620.971 kb on - strand at 621.070 kb on + strand at 621.070 kb on + strand at 621.070 kb on + strand at 621.070 kb on + strand at 621.118 kb on + strand at 621.118 kb on + strand at 621.118 kb on + strand at 621.126 kb on - strand at 621.126 kb on - strand at 621.149 kb on - strand at 621.208 kb on - strand at 621.223 kb on + strand at 621.231 kb on - strand at 621.279 kb on + strand at 621.349 kb on + strand, within SO0595 at 621.349 kb on + strand, within SO0595 at 621.349 kb on + strand, within SO0595 at 621.369 kb on - strand, within SO0595 at 621.371 kb on + strand, within SO0595 at 621.380 kb on - strand, within SO0595 at 621.432 kb on - strand, within SO0595 at 621.438 kb on + strand, within SO0595 at 621.443 kb on + strand, within SO0595 at 621.452 kb on - strand, within SO0595 at 621.452 kb on - strand, within SO0595 at 621.469 kb on - strand, within SO0595 at 621.471 kb on + strand, within SO0595 at 621.479 kb on - strand, within SO0595 at 621.483 kb on - strand, within SO0595 at 621.552 kb on + strand, within SO0595 at 621.570 kb on + strand, within SO0595 at 621.625 kb on - strand, within SO0595 at 621.655 kb on + strand, within SO0595 at 621.718 kb on - strand, within SO0595 at 621.733 kb on + strand, within SO0595 at 621.733 kb on + strand, within SO0595 at 621.741 kb on - strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.753 kb on + strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.761 kb on - strand, within SO0595 at 621.762 kb on - strand, within SO0595 at 621.796 kb on + strand at 621.803 kb on + strand at 621.803 kb on - strand at 621.804 kb on - strand at 621.809 kb on - strand at 621.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 620,313 + +0.2 620,606 + tRNA-Gly-2 SO_t011 0.50 +3.3 620,750 + +0.7 620,750 + -0.6 620,808 + tRNA-Gly-1 SO_t012 0.50 +0.7 620,936 - -0.1 620,950 - -0.0 620,951 - -0.4 620,953 + -0.0 620,953 + -0.6 620,953 + -0.9 620,953 + -0.9 620,953 + +0.2 620,958 + +0.5 620,958 + -0.5 620,961 - +0.3 620,961 - +0.3 620,961 - +0.3 620,966 - -0.6 620,966 - -0.0 620,971 - -0.0 621,070 + +0.2 621,070 + +0.6 621,070 + +0.8 621,070 + +1.1 621,118 + -0.0 621,118 + -0.3 621,118 + -0.9 621,126 - -0.4 621,126 - +1.3 621,149 - -0.8 621,208 - +0.0 621,223 + +1.0 621,231 - +1.0 621,279 + +0.6 621,349 + SO0595 0.12 -1.0 621,349 + SO0595 0.12 +0.6 621,349 + SO0595 0.12 +0.5 621,369 - SO0595 0.16 +0.1 621,371 + SO0595 0.16 +0.2 621,380 - SO0595 0.18 +1.0 621,432 - SO0595 0.27 +0.2 621,438 + SO0595 0.28 -0.4 621,443 + SO0595 0.29 +0.8 621,452 - SO0595 0.31 -0.5 621,452 - SO0595 0.31 -0.5 621,469 - SO0595 0.34 +0.4 621,471 + SO0595 0.34 +0.1 621,479 - SO0595 0.36 -0.4 621,483 - SO0595 0.36 -0.1 621,552 + SO0595 0.49 -1.0 621,570 + SO0595 0.52 -1.0 621,625 - SO0595 0.62 -0.4 621,655 + SO0595 0.68 +1.7 621,718 - SO0595 0.79 -0.3 621,733 + SO0595 0.82 +0.1 621,733 + SO0595 0.82 +0.3 621,741 - SO0595 0.83 -1.8 621,753 + SO0595 0.85 +1.6 621,753 + SO0595 0.85 +0.8 621,753 + SO0595 0.85 -0.0 621,753 + SO0595 0.85 -0.7 621,753 + SO0595 0.85 -0.2 621,753 + SO0595 0.85 -0.2 621,753 + SO0595 0.85 +0.1 621,753 + SO0595 0.85 -0.2 621,753 + SO0595 0.85 -1.0 621,753 + SO0595 0.85 -1.2 621,753 + SO0595 0.85 -0.8 621,753 + SO0595 0.85 +1.0 621,753 + SO0595 0.85 +1.0 621,753 + SO0595 0.85 -0.0 621,753 + SO0595 0.85 +0.4 621,753 + SO0595 0.85 -0.3 621,753 + SO0595 0.85 +0.7 621,753 + SO0595 0.85 -1.2 621,753 + SO0595 0.85 -0.8 621,753 + SO0595 0.85 +0.6 621,753 + SO0595 0.85 -0.2 621,753 + SO0595 0.85 -0.2 621,753 + SO0595 0.85 -1.5 621,753 + SO0595 0.85 -1.0 621,753 + SO0595 0.85 -0.1 621,753 + SO0595 0.85 -0.6 621,753 + SO0595 0.85 -0.0 621,753 + SO0595 0.85 +0.4 621,761 - SO0595 0.87 -1.4 621,761 - SO0595 0.87 +1.1 621,761 - SO0595 0.87 +0.1 621,761 - SO0595 0.87 -0.1 621,761 - SO0595 0.87 +0.2 621,761 - SO0595 0.87 -0.1 621,761 - SO0595 0.87 +0.1 621,761 - SO0595 0.87 +0.4 621,761 - SO0595 0.87 +0.8 621,761 - SO0595 0.87 +0.4 621,761 - SO0595 0.87 -0.6 621,761 - SO0595 0.87 -0.3 621,761 - SO0595 0.87 -0.0 621,761 - SO0595 0.87 -0.1 621,761 - SO0595 0.87 +0.0 621,761 - SO0595 0.87 +1.3 621,761 - SO0595 0.87 -1.5 621,761 - SO0595 0.87 +1.0 621,761 - SO0595 0.87 +0.7 621,761 - SO0595 0.87 -0.3 621,761 - SO0595 0.87 -1.5 621,761 - SO0595 0.87 +0.4 621,761 - SO0595 0.87 +0.8 621,761 - SO0595 0.87 +0.0 621,761 - SO0595 0.87 +0.1 621,761 - SO0595 0.87 -0.2 621,761 - SO0595 0.87 -0.4 621,761 - SO0595 0.87 -2.2 621,761 - SO0595 0.87 +0.2 621,762 - SO0595 0.87 -0.6 621,796 + +0.2 621,803 + +1.1 621,803 - +1.6 621,804 - -0.8 621,809 - -0.9 621,811 - +0.1
Or see this region's nucleotide sequence