Experiment: Varel_Bryant_medium_Glucose with Irgasan 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1459 and BT1460 are separated by 156 nucleotides BT1460 and BT1461 are separated by 868 nucleotides
BT1459: BT1459 - putative two-component system sensor histidine kinase, no kinase domain (NCBI ptt file), at 1,796,636 to 1,797,616
BT1459
BT1460: BT1460 - conserved hypothetical protein (NCBI ptt file), at 1,797,773 to 1,800,250
BT1460
BT1461: BT1461 - hypothetical protein (NCBI ptt file), at 1,801,119 to 1,801,463
BT1461
Position (kb)
1797
1798
1799
1800
1801 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1796.777 kb on - strand, within BT1459 at 1796.870 kb on - strand, within BT1459 at 1796.918 kb on + strand, within BT1459 at 1797.041 kb on + strand, within BT1459 at 1797.266 kb on + strand, within BT1459 at 1797.266 kb on + strand, within BT1459 at 1797.303 kb on + strand, within BT1459 at 1797.376 kb on - strand, within BT1459 at 1797.380 kb on + strand at 1797.380 kb on + strand, within BT1459 at 1797.380 kb on + strand, within BT1459 at 1797.436 kb on - strand, within BT1459 at 1797.444 kb on + strand, within BT1459 at 1797.507 kb on + strand, within BT1459 at 1797.508 kb on - strand, within BT1459 at 1797.508 kb on - strand, within BT1459 at 1797.518 kb on - strand, within BT1459 at 1797.556 kb on + strand at 1797.557 kb on - strand at 1797.667 kb on + strand at 1797.668 kb on - strand at 1797.669 kb on + strand at 1797.716 kb on - strand at 1797.761 kb on + strand at 1797.762 kb on - strand at 1797.775 kb on - strand at 1797.787 kb on + strand at 1797.787 kb on + strand at 1797.787 kb on + strand at 1797.799 kb on + strand at 1797.852 kb on + strand at 1797.853 kb on - strand at 1797.883 kb on + strand at 1797.884 kb on - strand at 1797.949 kb on + strand at 1798.162 kb on - strand, within BT1460 at 1798.190 kb on + strand, within BT1460 at 1798.190 kb on + strand, within BT1460 at 1798.332 kb on + strand, within BT1460 at 1798.333 kb on - strand, within BT1460 at 1798.405 kb on - strand, within BT1460 at 1798.413 kb on - strand, within BT1460 at 1798.444 kb on - strand, within BT1460 at 1798.514 kb on - strand, within BT1460 at 1798.574 kb on + strand, within BT1460 at 1798.715 kb on - strand, within BT1460 at 1798.720 kb on - strand, within BT1460 at 1798.809 kb on + strand, within BT1460 at 1798.822 kb on + strand, within BT1460 at 1798.823 kb on - strand, within BT1460 at 1798.825 kb on - strand, within BT1460 at 1798.865 kb on - strand, within BT1460 at 1798.918 kb on + strand, within BT1460 at 1798.919 kb on - strand, within BT1460 at 1798.920 kb on + strand, within BT1460 at 1798.921 kb on - strand, within BT1460 at 1798.921 kb on - strand, within BT1460 at 1798.921 kb on - strand, within BT1460 at 1799.039 kb on - strand, within BT1460 at 1799.053 kb on + strand, within BT1460 at 1799.054 kb on - strand, within BT1460 at 1799.055 kb on + strand, within BT1460 at 1799.214 kb on + strand, within BT1460 at 1799.278 kb on - strand, within BT1460 at 1799.278 kb on - strand, within BT1460 at 1799.288 kb on - strand, within BT1460 at 1799.541 kb on - strand, within BT1460 at 1799.698 kb on + strand, within BT1460 at 1799.698 kb on + strand, within BT1460 at 1799.699 kb on - strand, within BT1460 at 1799.884 kb on + strand, within BT1460 at 1799.987 kb on - strand, within BT1460 at 1800.080 kb on + strand at 1800.104 kb on + strand at 1800.169 kb on + strand at 1800.361 kb on - strand at 1800.393 kb on - strand at 1800.399 kb on - strand at 1800.462 kb on + strand at 1800.462 kb on + strand at 1800.463 kb on - strand at 1800.495 kb on + strand at 1800.816 kb on + strand at 1800.821 kb on + strand at 1800.834 kb on - strand at 1800.840 kb on + strand at 1800.845 kb on - strand at 1800.845 kb on - strand at 1800.869 kb on - strand at 1800.914 kb on - strand at 1800.916 kb on + strand at 1800.919 kb on - strand at 1800.920 kb on + strand at 1801.005 kb on + strand at 1801.010 kb on + strand at 1801.048 kb on + strand at 1801.048 kb on + strand at 1801.092 kb on + strand at 1801.183 kb on + strand, within BT1461 at 1801.207 kb on - strand, within BT1461 at 1801.217 kb on - strand, within BT1461 at 1801.232 kb on + strand, within BT1461
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Irgasan 0.025 mM remove 1,796,777 - BT1459 0.14 +0.1 1,796,870 - BT1459 0.24 -1.5 1,796,918 + BT1459 0.29 -1.6 1,797,041 + BT1459 0.41 -2.5 1,797,266 + BT1459 0.64 -0.9 1,797,266 + BT1459 0.64 -2.5 1,797,303 + BT1459 0.68 +0.5 1,797,376 - BT1459 0.75 +0.1 1,797,380 + +0.1 1,797,380 + BT1459 0.76 -1.5 1,797,380 + BT1459 0.76 -0.4 1,797,436 - BT1459 0.82 +0.1 1,797,444 + BT1459 0.82 +1.1 1,797,507 + BT1459 0.89 -1.5 1,797,508 - BT1459 0.89 +0.1 1,797,508 - BT1459 0.89 +0.3 1,797,518 - BT1459 0.90 +1.1 1,797,556 + -0.9 1,797,557 - -1.9 1,797,667 + +1.7 1,797,668 - -2.7 1,797,669 + +0.4 1,797,716 - -0.9 1,797,761 + +0.1 1,797,762 - +0.1 1,797,775 - -1.9 1,797,787 + +0.1 1,797,787 + +0.1 1,797,787 + -1.5 1,797,799 + -1.5 1,797,852 + -4.4 1,797,853 - -0.9 1,797,883 + -1.8 1,797,884 - -0.5 1,797,949 + -1.5 1,798,162 - BT1460 0.16 +0.6 1,798,190 + BT1460 0.17 -0.1 1,798,190 + BT1460 0.17 +0.1 1,798,332 + BT1460 0.23 -0.3 1,798,333 - BT1460 0.23 +1.2 1,798,405 - BT1460 0.26 +0.1 1,798,413 - BT1460 0.26 -0.6 1,798,444 - BT1460 0.27 -2.5 1,798,514 - BT1460 0.30 -0.6 1,798,574 + BT1460 0.32 -0.4 1,798,715 - BT1460 0.38 +2.1 1,798,720 - BT1460 0.38 -2.6 1,798,809 + BT1460 0.42 +0.1 1,798,822 + BT1460 0.42 -0.9 1,798,823 - BT1460 0.42 -2.4 1,798,825 - BT1460 0.42 -0.5 1,798,865 - BT1460 0.44 -1.1 1,798,918 + BT1460 0.46 +0.5 1,798,919 - BT1460 0.46 -0.2 1,798,920 + BT1460 0.46 -2.5 1,798,921 - BT1460 0.46 +1.7 1,798,921 - BT1460 0.46 -0.3 1,798,921 - BT1460 0.46 +0.1 1,799,039 - BT1460 0.51 -0.9 1,799,053 + BT1460 0.52 -0.2 1,799,054 - BT1460 0.52 -0.5 1,799,055 + BT1460 0.52 +1.1 1,799,214 + BT1460 0.58 -0.5 1,799,278 - BT1460 0.61 -2.2 1,799,278 - BT1460 0.61 -1.6 1,799,288 - BT1460 0.61 -1.2 1,799,541 - BT1460 0.71 -1.1 1,799,698 + BT1460 0.78 +2.0 1,799,698 + BT1460 0.78 +0.1 1,799,699 - BT1460 0.78 +0.1 1,799,884 + BT1460 0.85 +1.1 1,799,987 - BT1460 0.89 -0.0 1,800,080 + +1.1 1,800,104 + -1.5 1,800,169 + +0.1 1,800,361 - +2.1 1,800,393 - -2.4 1,800,399 - +1.1 1,800,462 + -0.5 1,800,462 + +0.3 1,800,463 - -1.9 1,800,495 + -0.1 1,800,816 + +0.1 1,800,821 + +1.1 1,800,834 - -1.9 1,800,840 + -1.9 1,800,845 - -0.3 1,800,845 - -1.2 1,800,869 - -1.9 1,800,914 - -0.3 1,800,916 + -1.6 1,800,919 - -1.6 1,800,920 + -0.9 1,801,005 + -0.5 1,801,010 + -1.4 1,801,048 + +1.1 1,801,048 + +0.1 1,801,092 + -0.9 1,801,183 + BT1461 0.19 -0.6 1,801,207 - BT1461 0.26 -1.3 1,801,217 - BT1461 0.28 +1.1 1,801,232 + BT1461 0.33 +0.8
Or see this region's nucleotide sequence