Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemC and hemD are separated by 4 nucleotides hemD and hemX are separated by 60 nucleotides hemX and SO4316 overlap by 4 nucleotides
SO4313: hemC - porphobilinogen deaminase (NCBI ptt file), at 4,490,624 to 4,491,556
hemC
SO4314: hemD - uroporphyrinogen-III synthase (NCBI ptt file), at 4,491,561 to 4,492,301
hemD
SO4315: hemX - hemX protein (NCBI ptt file), at 4,492,362 to 4,493,606
hemX
SO4316: SO4316 - hemY protein, putative (NCBI ptt file), at 4,493,603 to 4,494,769
SO4316
Position (kb)
4492
4493
4494 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4492.288 kb on + strand at 4492.529 kb on + strand, within hemX at 4492.598 kb on + strand, within hemX at 4492.629 kb on + strand, within hemX at 4492.630 kb on + strand, within hemX at 4492.630 kb on + strand, within hemX at 4492.630 kb on + strand, within hemX at 4492.638 kb on - strand, within hemX at 4492.638 kb on - strand, within hemX at 4492.648 kb on + strand, within hemX at 4492.842 kb on - strand, within hemX at 4492.864 kb on - strand, within hemX at 4492.880 kb on + strand, within hemX at 4492.885 kb on + strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.893 kb on - strand, within hemX at 4492.893 kb on - strand, within hemX at 4492.959 kb on + strand, within hemX at 4492.995 kb on + strand, within hemX at 4493.031 kb on + strand, within hemX at 4493.112 kb on - strand, within hemX at 4493.112 kb on - strand, within hemX at 4493.448 kb on - strand, within hemX at 4493.456 kb on - strand, within hemX at 4493.457 kb on + strand, within hemX at 4493.692 kb on - strand at 4493.764 kb on + strand, within SO4316 at 4493.807 kb on + strand, within SO4316 at 4493.839 kb on - strand, within SO4316 at 4493.865 kb on + strand, within SO4316 at 4493.940 kb on + strand, within SO4316 at 4494.107 kb on + strand, within SO4316 at 4494.109 kb on - strand, within SO4316 at 4494.112 kb on + strand, within SO4316 at 4494.164 kb on + strand, within SO4316 at 4494.177 kb on + strand, within SO4316 at 4494.185 kb on - strand, within SO4316 at 4494.185 kb on - strand, within SO4316 at 4494.190 kb on - strand, within SO4316 at 4494.210 kb on - strand, within SO4316 at 4494.292 kb on + strand, within SO4316 at 4494.292 kb on - strand, within SO4316 at 4494.336 kb on + strand, within SO4316 at 4494.344 kb on - strand, within SO4316 at 4494.374 kb on - strand, within SO4316 at 4494.410 kb on + strand, within SO4316 at 4494.418 kb on - strand, within SO4316
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 4,492,288 + +1.0 4,492,529 + hemX SO4315 0.13 +1.4 4,492,598 + hemX SO4315 0.19 -1.7 4,492,629 + hemX SO4315 0.21 +0.9 4,492,630 + hemX SO4315 0.22 +0.7 4,492,630 + hemX SO4315 0.22 +1.0 4,492,630 + hemX SO4315 0.22 -1.0 4,492,638 - hemX SO4315 0.22 -1.2 4,492,638 - hemX SO4315 0.22 -0.0 4,492,648 + hemX SO4315 0.23 +0.2 4,492,842 - hemX SO4315 0.39 +0.3 4,492,864 - hemX SO4315 0.40 +0.7 4,492,880 + hemX SO4315 0.42 -1.5 4,492,885 + hemX SO4315 0.42 -0.0 4,492,888 - hemX SO4315 0.42 +0.2 4,492,888 - hemX SO4315 0.42 +1.0 4,492,888 - hemX SO4315 0.42 +1.0 4,492,888 - hemX SO4315 0.42 +0.6 4,492,893 - hemX SO4315 0.43 -0.1 4,492,893 - hemX SO4315 0.43 +1.0 4,492,959 + hemX SO4315 0.48 -0.6 4,492,995 + hemX SO4315 0.51 +0.3 4,493,031 + hemX SO4315 0.54 -0.0 4,493,112 - hemX SO4315 0.60 +1.7 4,493,112 - hemX SO4315 0.60 +0.4 4,493,448 - hemX SO4315 0.87 +1.0 4,493,456 - hemX SO4315 0.88 +0.6 4,493,457 + hemX SO4315 0.88 +1.0 4,493,692 - +0.3 4,493,764 + SO4316 0.14 -1.3 4,493,807 + SO4316 0.17 +1.0 4,493,839 - SO4316 0.20 +1.0 4,493,865 + SO4316 0.22 -0.1 4,493,940 + SO4316 0.29 +0.6 4,494,107 + SO4316 0.43 -0.0 4,494,109 - SO4316 0.43 -0.5 4,494,112 + SO4316 0.44 +0.7 4,494,164 + SO4316 0.48 -2.1 4,494,177 + SO4316 0.49 -0.3 4,494,185 - SO4316 0.50 -0.3 4,494,185 - SO4316 0.50 +2.0 4,494,190 - SO4316 0.50 +2.0 4,494,210 - SO4316 0.52 -1.2 4,494,292 + SO4316 0.59 +1.2 4,494,292 - SO4316 0.59 -1.7 4,494,336 + SO4316 0.63 +0.7 4,494,344 - SO4316 0.63 +0.1 4,494,374 - SO4316 0.66 +0.1 4,494,410 + SO4316 0.69 +1.2 4,494,418 - SO4316 0.70 +1.0
Or see this region's nucleotide sequence