Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and cysJ are separated by 75 nucleotides cysJ and pntA are separated by 416 nucleotides
SO3737: cysI - sulfite reductase (NADPH) hemoprotein beta-component (cysI) (NCBI ptt file), at 3,880,961 to 3,882,658
cysI
SO3738: cysJ - sulfite reductase (NADPH) flavoprotein alpha-component (NCBI ptt file), at 3,882,734 to 3,884,557
cysJ
SO3740: pntA - NAD(P) transhydrogenase, alpha subunit (NCBI ptt file), at 3,884,974 to 3,886,500
pntA
Position (kb)
3882
3883
3884
3885 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3881.774 kb on - strand, within cysI at 3881.815 kb on - strand, within cysI at 3881.934 kb on - strand, within cysI at 3882.169 kb on + strand, within cysI at 3882.179 kb on - strand, within cysI at 3882.179 kb on - strand, within cysI at 3882.272 kb on + strand, within cysI at 3882.280 kb on - strand, within cysI at 3882.393 kb on - strand, within cysI at 3882.416 kb on - strand, within cysI at 3882.678 kb on - strand at 3882.765 kb on - strand at 3882.806 kb on - strand at 3882.847 kb on - strand at 3882.847 kb on - strand at 3882.927 kb on - strand, within cysJ at 3882.927 kb on - strand, within cysJ at 3883.497 kb on + strand, within cysJ at 3883.559 kb on - strand, within cysJ at 3883.574 kb on - strand, within cysJ at 3883.574 kb on - strand, within cysJ at 3883.658 kb on + strand, within cysJ at 3883.736 kb on + strand, within cysJ at 3883.744 kb on - strand, within cysJ at 3883.792 kb on - strand, within cysJ at 3883.956 kb on + strand, within cysJ at 3884.257 kb on - strand, within cysJ at 3884.257 kb on - strand, within cysJ at 3884.285 kb on - strand, within cysJ at 3884.373 kb on + strand, within cysJ at 3884.498 kb on - strand at 3884.577 kb on + strand at 3884.691 kb on + strand at 3884.729 kb on + strand at 3884.795 kb on - strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.900 kb on + strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.908 kb on - strand at 3884.921 kb on + strand at 3884.966 kb on - strand at 3885.038 kb on + strand at 3885.046 kb on - strand at 3885.062 kb on + strand at 3885.070 kb on - strand at 3885.141 kb on + strand, within pntA at 3885.153 kb on + strand, within pntA at 3885.229 kb on + strand, within pntA at 3885.239 kb on + strand, within pntA at 3885.327 kb on - strand, within pntA at 3885.344 kb on + strand, within pntA
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 3,881,774 - cysI SO3737 0.48 -1.2 3,881,815 - cysI SO3737 0.50 -1.0 3,881,934 - cysI SO3737 0.57 -1.4 3,882,169 + cysI SO3737 0.71 -1.4 3,882,179 - cysI SO3737 0.72 -1.2 3,882,179 - cysI SO3737 0.72 -2.1 3,882,272 + cysI SO3737 0.77 -0.7 3,882,280 - cysI SO3737 0.78 -1.7 3,882,393 - cysI SO3737 0.84 -2.5 3,882,416 - cysI SO3737 0.86 -1.3 3,882,678 - -0.1 3,882,765 - -0.6 3,882,806 - +0.5 3,882,847 - -3.1 3,882,847 - -1.1 3,882,927 - cysJ SO3738 0.11 -0.2 3,882,927 - cysJ SO3738 0.11 -2.8 3,883,497 + cysJ SO3738 0.42 +0.9 3,883,559 - cysJ SO3738 0.45 +0.9 3,883,574 - cysJ SO3738 0.46 -0.4 3,883,574 - cysJ SO3738 0.46 -0.9 3,883,658 + cysJ SO3738 0.51 -0.7 3,883,736 + cysJ SO3738 0.55 -0.1 3,883,744 - cysJ SO3738 0.55 -0.4 3,883,792 - cysJ SO3738 0.58 -0.7 3,883,956 + cysJ SO3738 0.67 -0.1 3,884,257 - cysJ SO3738 0.83 -0.8 3,884,257 - cysJ SO3738 0.83 +0.9 3,884,285 - cysJ SO3738 0.85 -0.4 3,884,373 + cysJ SO3738 0.90 -1.1 3,884,498 - +0.2 3,884,577 + -2.5 3,884,691 + +1.5 3,884,729 + +0.1 3,884,795 - -0.3 3,884,900 + -0.2 3,884,900 + +2.5 3,884,900 + -0.1 3,884,900 + +0.3 3,884,900 + -0.1 3,884,900 + -0.3 3,884,908 - +0.6 3,884,908 - +0.1 3,884,908 - -0.9 3,884,908 - +0.1 3,884,908 - -0.3 3,884,921 + -0.3 3,884,966 - -0.1 3,885,038 + -0.8 3,885,046 - +0.3 3,885,062 + +0.6 3,885,070 - +0.7 3,885,141 + pntA SO3740 0.11 -0.1 3,885,153 + pntA SO3740 0.12 +1.3 3,885,229 + pntA SO3740 0.17 -0.4 3,885,239 + pntA SO3740 0.17 -1.1 3,885,327 - pntA SO3740 0.23 -0.4 3,885,344 + pntA SO3740 0.24 +0.5
Or see this region's nucleotide sequence