Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3125 and ogt are separated by 37 nucleotides ogt and SO3127 are separated by 105 nucleotides
SO3125: SO3125 - ATP-dependent RNA helicase, DEAD box family (NCBI ptt file), at 3,252,401 to 3,253,684
SO3125
SO3126: ogt - methylated-DNA--protein-cysteine methyltransferase (NCBI ptt file), at 3,253,722 to 3,254,225
ogt
SO3127: SO3127 - Ada regulatory protein, putative (NCBI ptt file), at 3,254,331 to 3,255,959
SO3127
Position (kb)
3253
3254
3255 Strain fitness (log2 ratio)
-1
0
1
2 at 3252.815 kb on + strand, within SO3125 at 3252.833 kb on + strand, within SO3125 at 3252.836 kb on + strand, within SO3125 at 3253.121 kb on + strand, within SO3125 at 3253.139 kb on - strand, within SO3125 at 3253.338 kb on + strand, within SO3125 at 3253.440 kb on - strand, within SO3125 at 3253.456 kb on - strand, within SO3125 at 3253.582 kb on - strand at 3253.582 kb on - strand at 3253.589 kb on + strand at 3253.589 kb on + strand at 3253.886 kb on + strand, within ogt at 3253.897 kb on + strand, within ogt at 3254.038 kb on + strand, within ogt at 3254.230 kb on - strand at 3254.456 kb on + strand at 3254.458 kb on + strand at 3254.519 kb on + strand, within SO3127 at 3254.566 kb on + strand, within SO3127 at 3254.566 kb on + strand, within SO3127 at 3254.566 kb on + strand, within SO3127 at 3254.613 kb on + strand, within SO3127 at 3254.670 kb on + strand, within SO3127 at 3254.802 kb on - strand, within SO3127 at 3254.866 kb on + strand, within SO3127 at 3254.990 kb on + strand, within SO3127 at 3255.022 kb on - strand, within SO3127 at 3255.103 kb on + strand, within SO3127 at 3255.113 kb on + strand, within SO3127 at 3255.220 kb on + strand, within SO3127 at 3255.220 kb on + strand, within SO3127
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 3,252,815 + SO3125 0.32 +0.1 3,252,833 + SO3125 0.34 +1.4 3,252,836 + SO3125 0.34 -0.1 3,253,121 + SO3125 0.56 -0.3 3,253,139 - SO3125 0.57 -0.7 3,253,338 + SO3125 0.73 +0.5 3,253,440 - SO3125 0.81 -0.3 3,253,456 - SO3125 0.82 -1.1 3,253,582 - -0.3 3,253,582 - -0.6 3,253,589 + -0.0 3,253,589 + -0.1 3,253,886 + ogt SO3126 0.33 -0.7 3,253,897 + ogt SO3126 0.35 -0.5 3,254,038 + ogt SO3126 0.63 -0.7 3,254,230 - +0.3 3,254,456 + +0.2 3,254,458 + -0.1 3,254,519 + SO3127 0.12 -0.7 3,254,566 + SO3127 0.14 +0.3 3,254,566 + SO3127 0.14 -0.7 3,254,566 + SO3127 0.14 +0.0 3,254,613 + SO3127 0.17 +2.4 3,254,670 + SO3127 0.21 +1.3 3,254,802 - SO3127 0.29 -0.1 3,254,866 + SO3127 0.33 -1.1 3,254,990 + SO3127 0.40 +0.2 3,255,022 - SO3127 0.42 -1.1 3,255,103 + SO3127 0.47 -0.2 3,255,113 + SO3127 0.48 -0.7 3,255,220 + SO3127 0.55 +1.7 3,255,220 + SO3127 0.55 +0.3
Or see this region's nucleotide sequence