Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgm and msrA are separated by 110 nucleotides msrA and astE are separated by 278 nucleotides astE and SO2339 are separated by 162 nucleotides
SO2336: pgm - phosphoglucomutase, alpha-D-glucose phosphate-specific (NCBI ptt file), at 2,442,654 to 2,444,306
pgm
SO2337: msrA - peptide methionine sulfoxide reductase (NCBI ptt file), at 2,444,417 to 2,444,896
msrA
SO2338: astE - succinylglutamate desuccinylase (NCBI ptt file), at 2,445,175 to 2,446,209
astE
SO2339: SO2339 - alpha keto acid dehydrogenase complex, E1 component, alpha subunit (NCBI ptt file), at 2,446,372 to 2,447,550
SO2339
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-2
-1
0
1 at 2444.254 kb on - strand at 2444.403 kb on - strand at 2444.883 kb on + strand at 2444.991 kb on + strand at 2445.030 kb on + strand at 2445.074 kb on + strand at 2445.116 kb on - strand at 2445.343 kb on + strand, within astE at 2445.348 kb on + strand, within astE at 2445.527 kb on - strand, within astE at 2445.534 kb on + strand, within astE at 2445.560 kb on - strand, within astE at 2445.730 kb on - strand, within astE at 2445.732 kb on + strand, within astE at 2445.839 kb on + strand, within astE at 2445.863 kb on - strand, within astE at 2445.934 kb on + strand, within astE at 2446.184 kb on + strand at 2446.818 kb on - strand, within SO2339 at 2446.862 kb on + strand, within SO2339 at 2446.868 kb on - strand, within SO2339 at 2446.968 kb on - strand, within SO2339 at 2447.090 kb on - strand, within SO2339 at 2447.151 kb on + strand, within SO2339 at 2447.160 kb on - strand, within SO2339 at 2447.196 kb on + strand, within SO2339
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 2,444,254 - -0.3 2,444,403 - -0.1 2,444,883 + -0.2 2,444,991 + -0.8 2,445,030 + -0.6 2,445,074 + -0.3 2,445,116 - +1.7 2,445,343 + astE SO2338 0.16 -0.8 2,445,348 + astE SO2338 0.17 +1.7 2,445,527 - astE SO2338 0.34 -0.6 2,445,534 + astE SO2338 0.35 -1.1 2,445,560 - astE SO2338 0.37 -0.3 2,445,730 - astE SO2338 0.54 -2.3 2,445,732 + astE SO2338 0.54 -1.0 2,445,839 + astE SO2338 0.64 -0.5 2,445,863 - astE SO2338 0.66 -0.3 2,445,934 + astE SO2338 0.73 -0.8 2,446,184 + +0.7 2,446,818 - SO2339 0.38 -0.9 2,446,862 + SO2339 0.42 +1.7 2,446,868 - SO2339 0.42 +0.7 2,446,968 - SO2339 0.51 -0.3 2,447,090 - SO2339 0.61 +0.7 2,447,151 + SO2339 0.66 +0.7 2,447,160 - SO2339 0.67 +0.7 2,447,196 + SO2339 0.70 +1.7
Or see this region's nucleotide sequence