Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folB and folK-2 are separated by 42 nucleotides folK-2 and SO1293 are separated by 18 nucleotides SO1293 and SO1294 are separated by 84 nucleotides
SO1291: folB - dihydroneopterin aldolase (NCBI ptt file), at 1,342,213 to 1,342,563
folB
SO1292: folK-2 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 1,342,606 to 1,343,109
folK-2
SO1293: SO1293 - undecaprenol kinase, putative (NCBI ptt file), at 1,343,128 to 1,343,928
SO1293
SO1294: SO1294 - ribosomal RNA large subunit methyltransferase A, putative (NCBI ptt file), at 1,344,013 to 1,344,840
SO1294
Position (kb)
1343
1344 Strain fitness (log2 ratio)
-1
0
1
2 at 1342.152 kb on + strand at 1342.158 kb on + strand at 1342.176 kb on + strand at 1342.592 kb on + strand at 1342.592 kb on + strand at 1342.599 kb on + strand at 1342.599 kb on - strand at 1342.624 kb on + strand at 1342.645 kb on - strand at 1342.927 kb on + strand, within folK-2 at 1342.971 kb on - strand, within folK-2 at 1343.013 kb on + strand, within folK-2 at 1343.090 kb on + strand at 1343.161 kb on + strand at 1343.223 kb on + strand, within SO1293 at 1343.223 kb on + strand, within SO1293 at 1343.231 kb on - strand, within SO1293 at 1343.231 kb on - strand, within SO1293 at 1343.247 kb on - strand, within SO1293 at 1343.253 kb on - strand, within SO1293 at 1343.268 kb on + strand, within SO1293 at 1343.313 kb on + strand, within SO1293 at 1343.321 kb on - strand, within SO1293 at 1343.321 kb on - strand, within SO1293 at 1343.321 kb on - strand, within SO1293 at 1343.332 kb on - strand, within SO1293 at 1343.356 kb on + strand, within SO1293 at 1343.357 kb on - strand, within SO1293 at 1343.642 kb on + strand, within SO1293 at 1343.797 kb on + strand, within SO1293 at 1343.905 kb on - strand at 1343.910 kb on - strand at 1343.915 kb on - strand at 1344.017 kb on - strand at 1344.294 kb on - strand, within SO1294 at 1344.366 kb on + strand, within SO1294 at 1344.435 kb on + strand, within SO1294 at 1344.493 kb on + strand, within SO1294 at 1344.499 kb on - strand, within SO1294 at 1344.533 kb on + strand, within SO1294 at 1344.569 kb on - strand, within SO1294 at 1344.572 kb on - strand, within SO1294 at 1344.714 kb on - strand, within SO1294 at 1344.763 kb on + strand at 1344.860 kb on + strand at 1344.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 1,342,152 + -0.4 1,342,158 + +0.6 1,342,176 + -0.5 1,342,592 + -1.1 1,342,592 + +0.3 1,342,599 + +0.7 1,342,599 - -0.1 1,342,624 + -0.1 1,342,645 - +0.7 1,342,927 + folK-2 SO1292 0.64 -0.5 1,342,971 - folK-2 SO1292 0.72 -0.1 1,343,013 + folK-2 SO1292 0.81 -0.6 1,343,090 + +1.5 1,343,161 + +0.3 1,343,223 + SO1293 0.12 +0.2 1,343,223 + SO1293 0.12 -0.3 1,343,231 - SO1293 0.13 +0.3 1,343,231 - SO1293 0.13 +0.6 1,343,247 - SO1293 0.15 -1.1 1,343,253 - SO1293 0.16 +0.7 1,343,268 + SO1293 0.17 -0.8 1,343,313 + SO1293 0.23 +0.4 1,343,321 - SO1293 0.24 +0.2 1,343,321 - SO1293 0.24 +0.4 1,343,321 - SO1293 0.24 +0.3 1,343,332 - SO1293 0.25 -0.4 1,343,356 + SO1293 0.28 +1.3 1,343,357 - SO1293 0.29 +0.3 1,343,642 + SO1293 0.64 -0.8 1,343,797 + SO1293 0.84 +1.1 1,343,905 - +0.1 1,343,910 - -0.1 1,343,915 - -0.9 1,344,017 - -0.2 1,344,294 - SO1294 0.34 +0.1 1,344,366 + SO1294 0.43 +0.7 1,344,435 + SO1294 0.51 +0.1 1,344,493 + SO1294 0.58 -1.0 1,344,499 - SO1294 0.59 -0.1 1,344,533 + SO1294 0.63 +0.1 1,344,569 - SO1294 0.67 +1.9 1,344,572 - SO1294 0.68 -0.7 1,344,714 - SO1294 0.85 -1.1 1,344,763 + +0.1 1,344,860 + +0.2 1,344,869 - -1.0
Or see this region's nucleotide sequence