Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceE and aceF are separated by 12 nucleotides aceF and lpdA are separated by 149 nucleotides
SO0424: aceE - pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase (NCBI ptt file), at 438,400 to 441,066
aceE
SO0425: aceF - pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase (NCBI ptt file), at 441,079 to 443,112
aceF
SO0426: lpdA - pyruvate dehydrogenase complex, E3 component, lipoamide dehydrogenase (NCBI ptt file), at 443,262 to 444,689
lpdA
Position (kb) 441
442
443
444 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 440.246 kb on - strand, within aceE at 440.329 kb on + strand, within aceE at 440.346 kb on + strand, within aceE at 440.346 kb on + strand, within aceE at 440.521 kb on - strand, within aceE at 440.660 kb on + strand, within aceE at 440.758 kb on - strand, within aceE at 440.761 kb on + strand, within aceE at 440.854 kb on - strand at 440.869 kb on + strand at 440.869 kb on + strand at 440.877 kb on - strand at 440.877 kb on - strand at 440.879 kb on - strand at 440.903 kb on + strand at 441.048 kb on - strand at 441.151 kb on + strand at 441.298 kb on - strand, within aceF at 441.736 kb on - strand, within aceF at 441.875 kb on + strand, within aceF at 441.875 kb on + strand, within aceF at 441.875 kb on + strand, within aceF at 441.883 kb on - strand, within aceF at 441.962 kb on - strand, within aceF at 441.970 kb on - strand, within aceF at 442.179 kb on - strand, within aceF at 442.305 kb on - strand, within aceF at 442.645 kb on + strand, within aceF at 442.665 kb on - strand, within aceF at 442.741 kb on - strand, within aceF at 442.806 kb on - strand, within aceF at 442.830 kb on + strand, within aceF at 442.838 kb on - strand, within aceF at 442.919 kb on + strand at 442.927 kb on - strand at 443.016 kb on + strand at 443.028 kb on + strand at 443.063 kb on + strand at 443.133 kb on + strand at 443.136 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 440,246 - aceE SO0424 0.69 +2.0 440,329 + aceE SO0424 0.72 -0.7 440,346 + aceE SO0424 0.73 +0.6 440,346 + aceE SO0424 0.73 -0.2 440,521 - aceE SO0424 0.80 -0.9 440,660 + aceE SO0424 0.85 -0.2 440,758 - aceE SO0424 0.88 -0.4 440,761 + aceE SO0424 0.89 -2.5 440,854 - -2.4 440,869 + -1.7 440,869 + -1.3 440,877 - -0.3 440,877 - -0.7 440,879 - -1.6 440,903 + -1.2 441,048 - -1.6 441,151 + -2.2 441,298 - aceF SO0425 0.11 -0.9 441,736 - aceF SO0425 0.32 +0.4 441,875 + aceF SO0425 0.39 -2.0 441,875 + aceF SO0425 0.39 -1.6 441,875 + aceF SO0425 0.39 -0.7 441,883 - aceF SO0425 0.40 -2.4 441,962 - aceF SO0425 0.43 -0.2 441,970 - aceF SO0425 0.44 -0.2 442,179 - aceF SO0425 0.54 -0.3 442,305 - aceF SO0425 0.60 -2.3 442,645 + aceF SO0425 0.77 -1.0 442,665 - aceF SO0425 0.78 -0.5 442,741 - aceF SO0425 0.82 -2.0 442,806 - aceF SO0425 0.85 +0.6 442,830 + aceF SO0425 0.86 -0.0 442,838 - aceF SO0425 0.86 +0.5 442,919 + -2.8 442,927 - -1.0 443,016 + +0.3 443,028 + -0.0 443,063 + -2.0 443,133 + -0.1 443,136 - +0.1
Or see this region's nucleotide sequence