Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampE and pdhR are separated by 437 nucleotides pdhR and aceE are separated by 81 nucleotides
SO0422: ampE - AmpE protein (NCBI ptt file), at 436,277 to 437,128
ampE
SO0423: pdhR - pyruvate dehydrogenase complex repressor (NCBI ptt file), at 437,566 to 438,318
pdhR
SO0424: aceE - pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase (NCBI ptt file), at 438,400 to 441,066
aceE
Position (kb) 437
438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 436.595 kb on + strand, within ampE at 436.603 kb on - strand, within ampE at 436.603 kb on - strand, within ampE at 436.609 kb on + strand, within ampE at 436.617 kb on - strand, within ampE at 436.617 kb on - strand, within ampE at 436.622 kb on + strand, within ampE at 436.719 kb on - strand, within ampE at 436.768 kb on + strand, within ampE at 436.768 kb on + strand, within ampE at 436.776 kb on - strand, within ampE at 436.776 kb on - strand, within ampE at 436.776 kb on - strand, within ampE at 436.776 kb on - strand, within ampE at 436.904 kb on - strand, within ampE at 436.911 kb on - strand, within ampE at 436.911 kb on - strand, within ampE at 436.915 kb on + strand, within ampE at 436.915 kb on + strand, within ampE at 436.915 kb on + strand, within ampE at 436.946 kb on - strand, within ampE at 436.974 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 436.996 kb on + strand, within ampE at 437.004 kb on - strand, within ampE at 437.004 kb on - strand, within ampE at 437.078 kb on - strand at 437.088 kb on - strand at 437.170 kb on + strand at 437.170 kb on + strand at 437.275 kb on + strand at 437.290 kb on - strand at 437.323 kb on - strand at 437.365 kb on + strand at 437.373 kb on - strand at 437.397 kb on + strand at 437.430 kb on + strand at 437.438 kb on - strand at 437.645 kb on + strand, within pdhR at 437.645 kb on + strand, within pdhR at 437.645 kb on + strand, within pdhR at 437.653 kb on - strand, within pdhR at 437.653 kb on - strand, within pdhR at 437.726 kb on - strand, within pdhR at 437.924 kb on - strand, within pdhR at 437.964 kb on + strand, within pdhR at 438.034 kb on + strand, within pdhR at 438.095 kb on - strand, within pdhR at 438.156 kb on + strand, within pdhR at 438.226 kb on + strand, within pdhR at 438.268 kb on + strand at 438.493 kb on - strand at 438.505 kb on - strand at 438.576 kb on + strand at 438.718 kb on + strand, within aceE at 438.915 kb on + strand, within aceE at 438.982 kb on + strand, within aceE at 439.112 kb on + strand, within aceE at 439.212 kb on + strand, within aceE at 439.256 kb on + strand, within aceE
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 436,595 + ampE SO0422 0.37 +0.4 436,603 - ampE SO0422 0.38 +2.0 436,603 - ampE SO0422 0.38 -1.3 436,609 + ampE SO0422 0.39 +1.0 436,617 - ampE SO0422 0.40 +0.5 436,617 - ampE SO0422 0.40 -0.4 436,622 + ampE SO0422 0.40 -0.3 436,719 - ampE SO0422 0.52 +1.4 436,768 + ampE SO0422 0.58 -0.2 436,768 + ampE SO0422 0.58 -0.9 436,776 - ampE SO0422 0.59 +0.2 436,776 - ampE SO0422 0.59 +0.3 436,776 - ampE SO0422 0.59 +0.3 436,776 - ampE SO0422 0.59 -1.4 436,904 - ampE SO0422 0.74 -0.4 436,911 - ampE SO0422 0.74 -1.6 436,911 - ampE SO0422 0.74 +1.0 436,915 + ampE SO0422 0.75 +1.1 436,915 + ampE SO0422 0.75 +1.0 436,915 + ampE SO0422 0.75 +0.8 436,946 - ampE SO0422 0.79 +1.0 436,974 + ampE SO0422 0.82 +0.3 436,996 + ampE SO0422 0.84 +1.1 436,996 + ampE SO0422 0.84 -0.4 436,996 + ampE SO0422 0.84 +0.7 436,996 + ampE SO0422 0.84 +0.4 437,004 - ampE SO0422 0.85 -0.2 437,004 - ampE SO0422 0.85 +0.3 437,078 - +1.8 437,088 - -0.3 437,170 + +0.7 437,170 + -0.5 437,275 + +0.1 437,290 - +0.1 437,323 - +0.0 437,365 + +0.3 437,373 - +1.0 437,397 + -0.4 437,430 + +0.8 437,438 - -0.4 437,645 + pdhR SO0423 0.10 -0.9 437,645 + pdhR SO0423 0.10 -2.2 437,645 + pdhR SO0423 0.10 -1.9 437,653 - pdhR SO0423 0.12 -0.3 437,653 - pdhR SO0423 0.12 -1.0 437,726 - pdhR SO0423 0.21 -0.6 437,924 - pdhR SO0423 0.48 +2.0 437,964 + pdhR SO0423 0.53 -0.0 438,034 + pdhR SO0423 0.62 -1.1 438,095 - pdhR SO0423 0.70 -1.9 438,156 + pdhR SO0423 0.78 +1.0 438,226 + pdhR SO0423 0.88 -0.0 438,268 + -0.7 438,493 - +0.5 438,505 - -1.6 438,576 + -0.6 438,718 + aceE SO0424 0.12 -1.6 438,915 + aceE SO0424 0.19 -1.5 438,982 + aceE SO0424 0.22 -1.6 439,112 + aceE SO0424 0.27 -2.8 439,212 + aceE SO0424 0.30 +1.4 439,256 + aceE SO0424 0.32 -0.8
Or see this region's nucleotide sequence