Experiment: minimal media, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdA and frdB overlap by 1 nucleotides frdB and SO0400 are separated by 63 nucleotides SO0400 and SO0401 overlap by 4 nucleotides
SO0398: frdA - fumarate reductase flavoprotein subunit (NCBI ptt file), at 411,381 to 413,387
frdA
SO0399: frdB - fumarate reductase iron-sulfur protein (NCBI ptt file), at 413,387 to 414,118
frdB
SO0400: SO0400 - conserved hypothetical protein (NCBI ptt file), at 414,182 to 414,466
SO0400
SO0401: SO0401 - alcohol dehydrogenase, zinc-containing (NCBI ptt file), at 414,463 to 415,476
SO0401
Position (kb) 414
415 Strain fitness (log2 ratio) -2
-1
0
1 at 413.196 kb on - strand at 413.206 kb on - strand at 413.301 kb on - strand at 413.313 kb on - strand at 413.331 kb on + strand at 413.397 kb on + strand at 413.460 kb on - strand at 413.573 kb on - strand, within frdB at 413.669 kb on - strand, within frdB at 413.750 kb on - strand, within frdB at 413.798 kb on - strand, within frdB at 413.845 kb on - strand, within frdB at 413.855 kb on - strand, within frdB at 413.861 kb on - strand, within frdB at 413.902 kb on - strand, within frdB at 414.008 kb on + strand, within frdB at 414.016 kb on - strand, within frdB at 414.056 kb on + strand at 414.056 kb on + strand at 414.064 kb on - strand at 414.178 kb on + strand at 414.204 kb on + strand at 414.246 kb on + strand, within SO0400 at 414.261 kb on - strand, within SO0400 at 414.317 kb on - strand, within SO0400 at 414.372 kb on + strand, within SO0400 at 414.412 kb on + strand, within SO0400 at 414.419 kb on + strand, within SO0400 at 414.509 kb on - strand at 414.565 kb on - strand, within SO0401 at 414.636 kb on - strand, within SO0401 at 414.668 kb on - strand, within SO0401 at 414.679 kb on - strand, within SO0401 at 414.681 kb on - strand, within SO0401 at 414.728 kb on - strand, within SO0401 at 414.848 kb on + strand, within SO0401 at 414.848 kb on + strand, within SO0401 at 414.856 kb on - strand, within SO0401 at 414.894 kb on + strand, within SO0401 at 414.910 kb on - strand, within SO0401 at 414.910 kb on - strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.954 kb on + strand, within SO0401 at 414.962 kb on - strand, within SO0401 at 414.962 kb on - strand, within SO0401 at 414.962 kb on - strand, within SO0401 at 415.092 kb on + strand, within SO0401 at 415.092 kb on + strand, within SO0401 at 415.100 kb on - strand, within SO0401 at 415.102 kb on + strand, within SO0401 at 415.116 kb on - strand, within SO0401 at 415.125 kb on + strand, within SO0401 at 415.132 kb on + strand, within SO0401 at 415.133 kb on - strand, within SO0401 at 415.138 kb on + strand, within SO0401 at 415.139 kb on - strand, within SO0401 at 415.165 kb on - strand, within SO0401 at 415.369 kb on + strand, within SO0401 at 415.463 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 1G configuration remove 413,196 - +0.3 413,206 - -0.4 413,301 - -0.7 413,313 - -0.9 413,331 + -0.1 413,397 + +0.2 413,460 - +0.2 413,573 - frdB SO0399 0.25 -0.0 413,669 - frdB SO0399 0.39 -0.1 413,750 - frdB SO0399 0.50 -0.3 413,798 - frdB SO0399 0.56 -0.4 413,845 - frdB SO0399 0.63 +0.1 413,855 - frdB SO0399 0.64 -0.2 413,861 - frdB SO0399 0.65 -0.3 413,902 - frdB SO0399 0.70 +0.2 414,008 + frdB SO0399 0.85 +0.7 414,016 - frdB SO0399 0.86 -1.3 414,056 + -0.1 414,056 + +1.6 414,064 - -0.0 414,178 + -0.3 414,204 + +0.3 414,246 + SO0400 0.22 -0.0 414,261 - SO0400 0.28 +0.4 414,317 - SO0400 0.47 -2.0 414,372 + SO0400 0.67 +0.9 414,412 + SO0400 0.81 +0.9 414,419 + SO0400 0.83 +1.7 414,509 - -0.8 414,565 - SO0401 0.10 -0.7 414,636 - SO0401 0.17 +0.1 414,668 - SO0401 0.20 -0.7 414,679 - SO0401 0.21 +0.5 414,681 - SO0401 0.21 +0.3 414,728 - SO0401 0.26 -0.8 414,848 + SO0401 0.38 -0.2 414,848 + SO0401 0.38 +0.6 414,856 - SO0401 0.39 -0.4 414,894 + SO0401 0.43 +0.1 414,910 - SO0401 0.44 +0.4 414,910 - SO0401 0.44 +0.1 414,954 + SO0401 0.48 +0.4 414,954 + SO0401 0.48 +0.5 414,954 + SO0401 0.48 +0.2 414,954 + SO0401 0.48 -0.0 414,962 - SO0401 0.49 +0.7 414,962 - SO0401 0.49 -0.3 414,962 - SO0401 0.49 -1.7 415,092 + SO0401 0.62 +0.3 415,092 + SO0401 0.62 +0.1 415,100 - SO0401 0.63 -0.0 415,102 + SO0401 0.63 -0.2 415,116 - SO0401 0.64 -0.1 415,125 + SO0401 0.65 -0.6 415,132 + SO0401 0.66 +0.2 415,133 - SO0401 0.66 +0.3 415,138 + SO0401 0.67 -0.1 415,139 - SO0401 0.67 -0.1 415,165 - SO0401 0.69 +1.0 415,369 + SO0401 0.89 -0.3 415,463 + +0.6
Or see this region's nucleotide sequence