Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1319 and HP15_1320 are separated by 105 nucleotides HP15_1320 and HP15_1321 are separated by 210 nucleotides HP15_1321 and HP15_tRNA22 are separated by 126 nucleotides HP15_tRNA22 and HP15_tRNA23 are separated by 64 nucleotides HP15_tRNA23 and HP15_tRNA24 are separated by 25 nucleotides
HP15_1319: HP15_1319 - alanyl-tRNA synthetase, class IIc, at 1,380,978 to 1,383,608
_1319
HP15_1320: HP15_1320 - aspartate kinase: aspartate kinase, monofunctional class, at 1,383,714 to 1,384,952
_1320
HP15_1321: HP15_1321 - carbon storage regulator, at 1,385,163 to 1,385,339
_1321
HP15_tRNA22: HP15_tRNA22 - tRNA-Ser, at 1,385,466 to 1,385,555
_tRNA22
HP15_tRNA23: HP15_tRNA23 - tRNA-Arg, at 1,385,620 to 1,385,696
_tRNA23
HP15_tRNA24: HP15_tRNA24 - tRNA-Arg, at 1,385,722 to 1,385,798
_tRNA24
Position (kb) 1383
1384
1385 Strain fitness (log2 ratio) -1
0
1
2 at 1385.119 kb on + strand at 1385.308 kb on + strand, within HP15_1321 at 1385.850 kb on + strand at 1385.908 kb on + strand at 1385.919 kb on + strand at 1385.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 1,385,119 + +1.5 1,385,308 + HP15_1321 0.82 -0.9 1,385,850 + +0.3 1,385,908 + -0.6 1,385,919 + +2.0 1,385,948 - -0.4
Or see this region's nucleotide sequence