Experiment: LB, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valS and tRNA-Arg-8 are separated by 601 nucleotides tRNA-Arg-8 and tRNA-Ser-4 are separated by 95 nucleotides tRNA-Ser-4 and tRNA-Arg-7 are separated by 36 nucleotides tRNA-Arg-7 and tRNA-Arg-6 are separated by 43 nucleotides tRNA-Arg-6 and tRNA-Arg-5 are separated by 43 nucleotides tRNA-Arg-5 and tRNA-Arg-4 are separated by 83 nucleotides tRNA-Arg-4 and tRNA-Arg-3 are separated by 31 nucleotides tRNA-Arg-3 and tRNA-Ser-3 are separated by 20 nucleotides
SO3424: valS - valyl-tRNA synthetase (NCBI ptt file), at 3,563,491 to 3,566,367
valS
SO_t083: tRNA-Arg-8 - tRNA-Arg (RefSeq), at 3,566,969 to 3,567,045
tRNA-Arg-8
SO_t084: tRNA-Ser-4 - tRNA-Ser (RefSeq), at 3,567,141 to 3,567,232
tRNA-Ser-4
SO_t085: tRNA-Arg-7 - tRNA-Arg (RefSeq), at 3,567,269 to 3,567,345
tRNA-Arg-7
SO_t086: tRNA-Arg-6 - tRNA-Arg (RefSeq), at 3,567,389 to 3,567,465
tRNA-Arg-6
SO_t087: tRNA-Arg-5 - tRNA-Arg (RefSeq), at 3,567,509 to 3,567,585
tRNA-Arg-5
SO_t088: tRNA-Arg-4 - tRNA-Arg (RefSeq), at 3,567,669 to 3,567,745
tRNA-Arg-4
SO_t089: tRNA-Arg-3 - tRNA-Arg (RefSeq), at 3,567,777 to 3,567,853
tRNA-Arg-3
SO_t090: tRNA-Ser-3 - tRNA-Ser (RefSeq), at 3,567,874 to 3,567,965
tRNA-Ser-3
Position (kb)
3566
3567
3568 Strain fitness (log2 ratio)
-1
0
1 at 3566.533 kb on + strand at 3566.541 kb on - strand at 3566.654 kb on + strand at 3566.662 kb on - strand at 3566.694 kb on - strand at 3566.694 kb on - strand at 3566.694 kb on - strand at 3566.701 kb on + strand at 3566.704 kb on - strand at 3566.709 kb on - strand at 3566.709 kb on - strand at 3566.716 kb on - strand at 3566.718 kb on - strand at 3566.830 kb on + strand at 3566.840 kb on - strand at 3566.840 kb on - strand at 3566.840 kb on - strand at 3566.840 kb on - strand at 3566.993 kb on - strand, within tRNA-Arg-8
Per-strain Table
Position Strand Gene LocusTag Fraction LB, aerobic, 1G configuration remove 3,566,533 + +0.4 3,566,541 - +0.1 3,566,654 + +0.3 3,566,662 - +0.6 3,566,694 - +0.1 3,566,694 - -0.6 3,566,694 - -0.0 3,566,701 + -0.6 3,566,704 - +0.3 3,566,709 - -0.4 3,566,709 - -0.0 3,566,716 - +0.0 3,566,718 - +0.2 3,566,830 + +1.3 3,566,840 - -0.4 3,566,840 - +0.4 3,566,840 - -0.3 3,566,840 - +0.2 3,566,993 - tRNA-Arg-8 SO_t083 0.31 -0.8
Or see this region's nucleotide sequence