Experiment: LB, aerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoL and nuoK overlap by 4 nucleotides nuoK and nuoJ overlap by 4 nucleotides nuoJ and nuoI are separated by 10 nucleotides
SO1011: nuoL - NADH dehydrogenase I, L subunit (NCBI ptt file), at 1,048,384 to 1,050,231
nuoL
SO1012: nuoK - NADH dehydrogenase I, K subunit (NCBI ptt file), at 1,050,228 to 1,050,536
nuoK
SO1013: nuoJ - NADH dehydrogenase I, J subunit (NCBI ptt file), at 1,050,533 to 1,051,084
nuoJ
SO1014: nuoI - NADH dehydrogenase I, I subunit (NCBI ptt file), at 1,051,095 to 1,051,637
nuoI
Position (kb)
1050
1051 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1049.249 kb on + strand, within nuoL at 1049.329 kb on - strand, within nuoL at 1049.354 kb on + strand, within nuoL at 1049.357 kb on - strand, within nuoL at 1049.451 kb on + strand, within nuoL at 1049.581 kb on - strand, within nuoL at 1049.581 kb on - strand, within nuoL at 1049.583 kb on + strand, within nuoL at 1049.583 kb on + strand, within nuoL at 1049.583 kb on + strand, within nuoL at 1049.591 kb on - strand, within nuoL at 1049.637 kb on + strand, within nuoL at 1049.652 kb on + strand, within nuoL at 1049.697 kb on - strand at 1049.725 kb on - strand, within nuoL at 1049.725 kb on - strand, within nuoL at 1049.748 kb on + strand, within nuoL at 1049.774 kb on + strand, within nuoL at 1049.792 kb on - strand, within nuoL at 1050.114 kb on + strand at 1050.114 kb on + strand at 1050.122 kb on - strand at 1050.133 kb on + strand at 1050.178 kb on - strand at 1050.183 kb on - strand at 1050.261 kb on - strand, within nuoK at 1050.285 kb on - strand, within nuoK at 1050.315 kb on - strand, within nuoK at 1050.371 kb on - strand, within nuoK at 1050.459 kb on + strand, within nuoK at 1050.467 kb on - strand, within nuoK at 1050.481 kb on + strand, within nuoK at 1050.481 kb on + strand, within nuoK at 1050.503 kb on + strand, within nuoK at 1050.503 kb on + strand, within nuoK at 1050.511 kb on - strand at 1050.543 kb on + strand at 1050.587 kb on - strand at 1050.589 kb on + strand, within nuoJ at 1050.605 kb on - strand, within nuoJ at 1050.696 kb on + strand, within nuoJ at 1050.706 kb on + strand, within nuoJ at 1050.706 kb on + strand, within nuoJ at 1050.714 kb on - strand, within nuoJ at 1051.006 kb on + strand, within nuoJ at 1051.155 kb on - strand, within nuoI at 1051.318 kb on + strand, within nuoI at 1051.446 kb on - strand, within nuoI at 1051.454 kb on - strand, within nuoI at 1051.485 kb on + strand, within nuoI at 1051.485 kb on + strand, within nuoI at 1051.493 kb on - strand, within nuoI
Per-strain Table
Position Strand Gene LocusTag Fraction LB, aerobic, 1G configuration remove 1,049,249 + nuoL SO1011 0.47 -0.3 1,049,329 - nuoL SO1011 0.51 +0.2 1,049,354 + nuoL SO1011 0.52 -0.4 1,049,357 - nuoL SO1011 0.53 +0.7 1,049,451 + nuoL SO1011 0.58 +0.5 1,049,581 - nuoL SO1011 0.65 -0.6 1,049,581 - nuoL SO1011 0.65 -0.7 1,049,583 + nuoL SO1011 0.65 +0.1 1,049,583 + nuoL SO1011 0.65 +0.0 1,049,583 + nuoL SO1011 0.65 -0.2 1,049,591 - nuoL SO1011 0.65 -0.5 1,049,637 + nuoL SO1011 0.68 -0.6 1,049,652 + nuoL SO1011 0.69 -1.5 1,049,697 - +2.0 1,049,725 - nuoL SO1011 0.73 -0.3 1,049,725 - nuoL SO1011 0.73 -0.5 1,049,748 + nuoL SO1011 0.74 -0.3 1,049,774 + nuoL SO1011 0.75 -0.8 1,049,792 - nuoL SO1011 0.76 -0.1 1,050,114 + -0.5 1,050,114 + -1.5 1,050,122 - +0.1 1,050,133 + -0.7 1,050,178 - +1.0 1,050,183 - +1.0 1,050,261 - nuoK SO1012 0.11 +2.0 1,050,285 - nuoK SO1012 0.18 -1.0 1,050,315 - nuoK SO1012 0.28 -1.2 1,050,371 - nuoK SO1012 0.46 -0.9 1,050,459 + nuoK SO1012 0.75 -0.1 1,050,467 - nuoK SO1012 0.77 +0.5 1,050,481 + nuoK SO1012 0.82 -1.0 1,050,481 + nuoK SO1012 0.82 -0.4 1,050,503 + nuoK SO1012 0.89 +0.3 1,050,503 + nuoK SO1012 0.89 -1.0 1,050,511 - -0.4 1,050,543 + -0.3 1,050,587 - +0.2 1,050,589 + nuoJ SO1013 0.10 +1.0 1,050,605 - nuoJ SO1013 0.13 -0.7 1,050,696 + nuoJ SO1013 0.30 -1.7 1,050,706 + nuoJ SO1013 0.31 -3.0 1,050,706 + nuoJ SO1013 0.31 +1.0 1,050,714 - nuoJ SO1013 0.33 +0.5 1,051,006 + nuoJ SO1013 0.86 -0.3 1,051,155 - nuoI SO1014 0.11 -0.7 1,051,318 + nuoI SO1014 0.41 -0.7 1,051,446 - nuoI SO1014 0.65 -1.1 1,051,454 - nuoI SO1014 0.66 +0.0 1,051,485 + nuoI SO1014 0.72 -0.7 1,051,485 + nuoI SO1014 0.72 -0.8 1,051,493 - nuoI SO1014 0.73 +0.4
Or see this region's nucleotide sequence