Experiment: LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08010 and BWI76_RS08015 are separated by 91 nucleotides BWI76_RS08015 and BWI76_RS08020 are separated by 106 nucleotides BWI76_RS08020 and BWI76_RS08025 are separated by 142 nucleotides
BWI76_RS08010: BWI76_RS08010 - octanoyltransferase, at 1,698,466 to 1,699,110
_RS08010
BWI76_RS08015: BWI76_RS08015 - hypothetical protein, at 1,699,202 to 1,699,465
_RS08015
BWI76_RS08020: BWI76_RS08020 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,699,572 to 1,700,771
_RS08020
BWI76_RS08025: BWI76_RS08025 - septal ring lytic transglycosylase RlpA, at 1,700,914 to 1,702,014
_RS08025
Position (kb)
1699
1700
1701 Strain fitness (log2 ratio)
-1
0
1 at 1699.151 kb on + strand at 1699.755 kb on - strand, within BWI76_RS08020 at 1700.122 kb on - strand, within BWI76_RS08020 at 1700.205 kb on + strand, within BWI76_RS08020 at 1700.215 kb on + strand, within BWI76_RS08020 at 1700.223 kb on - strand, within BWI76_RS08020 at 1700.259 kb on - strand, within BWI76_RS08020 at 1700.467 kb on + strand, within BWI76_RS08020 at 1700.504 kb on + strand, within BWI76_RS08020 at 1700.504 kb on + strand, within BWI76_RS08020 at 1700.707 kb on + strand at 1700.941 kb on + strand at 1700.949 kb on - strand at 1701.202 kb on + strand, within BWI76_RS08025 at 1701.311 kb on - strand, within BWI76_RS08025 at 1701.437 kb on + strand, within BWI76_RS08025 at 1701.525 kb on + strand, within BWI76_RS08025 at 1701.700 kb on - strand, within BWI76_RS08025
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,699,151 + +0.2 1,699,755 - BWI76_RS08020 0.15 -0.3 1,700,122 - BWI76_RS08020 0.46 +0.2 1,700,205 + BWI76_RS08020 0.53 -0.3 1,700,215 + BWI76_RS08020 0.54 +0.3 1,700,223 - BWI76_RS08020 0.54 -1.1 1,700,259 - BWI76_RS08020 0.57 -1.0 1,700,467 + BWI76_RS08020 0.75 -1.1 1,700,504 + BWI76_RS08020 0.78 -1.2 1,700,504 + BWI76_RS08020 0.78 -1.4 1,700,707 + -0.1 1,700,941 + -0.8 1,700,949 - +0.3 1,701,202 + BWI76_RS08025 0.26 -0.6 1,701,311 - BWI76_RS08025 0.36 +0.3 1,701,437 + BWI76_RS08025 0.48 +0.1 1,701,525 + BWI76_RS08025 0.55 +0.8 1,701,700 - BWI76_RS08025 0.71 -0.8
Or see this region's nucleotide sequence