Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4724 and Psyr_4725 are separated by 163 nucleotides Psyr_4725 and Psyr_4726 are separated by 190 nucleotides
Psyr_4724: Psyr_4724 - Phospholipid/glycerol acyltransferase, at 5,602,632 to 5,603,555
_4724
Psyr_4725: Psyr_4725 - Protein of unknown function DUF785, at 5,603,719 to 5,604,222
_4725
Psyr_4726: Psyr_4726 - hypothetical protein, at 5,604,413 to 5,605,573
_4726
Position (kb) 5603
5604
5605 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5602.725 kb on + strand, within Psyr_4724 at 5602.726 kb on - strand, within Psyr_4724 at 5602.726 kb on - strand, within Psyr_4724 at 5602.852 kb on + strand, within Psyr_4724 at 5603.003 kb on - strand, within Psyr_4724 at 5603.275 kb on + strand, within Psyr_4724 at 5603.275 kb on + strand, within Psyr_4724 at 5603.276 kb on - strand, within Psyr_4724 at 5603.276 kb on - strand, within Psyr_4724 at 5603.276 kb on - strand, within Psyr_4724 at 5603.276 kb on - strand, within Psyr_4724 at 5603.475 kb on - strand at 5603.475 kb on - strand at 5603.475 kb on - strand at 5603.485 kb on - strand at 5603.556 kb on + strand at 5603.556 kb on + strand at 5603.557 kb on - strand at 5603.560 kb on - strand at 5603.560 kb on - strand at 5603.585 kb on + strand at 5603.585 kb on + strand at 5603.586 kb on - strand at 5603.586 kb on - strand at 5603.586 kb on - strand at 5603.593 kb on + strand at 5603.593 kb on + strand at 5603.593 kb on + strand at 5603.593 kb on + strand at 5603.593 kb on + strand at 5603.593 kb on + strand at 5603.594 kb on - strand at 5603.594 kb on - strand at 5603.594 kb on - strand at 5603.594 kb on - strand at 5603.597 kb on + strand at 5603.598 kb on - strand at 5603.622 kb on + strand at 5603.623 kb on - strand at 5603.639 kb on + strand at 5603.639 kb on + strand at 5603.639 kb on + strand at 5603.639 kb on + strand at 5603.640 kb on - strand at 5603.640 kb on - strand at 5603.640 kb on - strand at 5603.640 kb on - strand at 5603.653 kb on + strand at 5603.671 kb on + strand at 5603.671 kb on + strand at 5603.700 kb on - strand at 5603.738 kb on + strand at 5603.785 kb on + strand, within Psyr_4725 at 5603.785 kb on + strand, within Psyr_4725 at 5603.785 kb on + strand, within Psyr_4725 at 5603.785 kb on + strand, within Psyr_4725 at 5603.785 kb on + strand, within Psyr_4725 at 5603.785 kb on + strand, within Psyr_4725 at 5603.785 kb on + strand, within Psyr_4725 at 5603.786 kb on - strand, within Psyr_4725 at 5603.786 kb on - strand, within Psyr_4725 at 5603.786 kb on - strand, within Psyr_4725 at 5603.786 kb on - strand, within Psyr_4725 at 5603.786 kb on - strand, within Psyr_4725 at 5603.919 kb on - strand, within Psyr_4725 at 5603.959 kb on + strand, within Psyr_4725 at 5603.959 kb on + strand, within Psyr_4725 at 5603.959 kb on + strand, within Psyr_4725 at 5603.959 kb on + strand, within Psyr_4725 at 5603.959 kb on + strand, within Psyr_4725 at 5603.960 kb on - strand, within Psyr_4725 at 5603.960 kb on - strand, within Psyr_4725 at 5603.960 kb on - strand, within Psyr_4725 at 5603.960 kb on - strand, within Psyr_4725 at 5603.960 kb on - strand, within Psyr_4725 at 5603.960 kb on - strand, within Psyr_4725 at 5604.091 kb on - strand, within Psyr_4725 at 5604.091 kb on - strand, within Psyr_4725 at 5604.091 kb on - strand, within Psyr_4725 at 5604.197 kb on - strand at 5604.198 kb on + strand at 5604.199 kb on - strand at 5604.232 kb on + strand at 5604.232 kb on + strand at 5605.137 kb on + strand, within Psyr_4726
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 5,602,725 + Psyr_4724 0.10 +1.3 5,602,726 - Psyr_4724 0.10 -0.4 5,602,726 - Psyr_4724 0.10 -0.5 5,602,852 + Psyr_4724 0.24 +1.4 5,603,003 - Psyr_4724 0.40 +0.5 5,603,275 + Psyr_4724 0.70 +0.2 5,603,275 + Psyr_4724 0.70 +0.7 5,603,276 - Psyr_4724 0.70 +0.3 5,603,276 - Psyr_4724 0.70 +0.2 5,603,276 - Psyr_4724 0.70 +0.4 5,603,276 - Psyr_4724 0.70 +0.6 5,603,475 - +0.8 5,603,475 - -2.2 5,603,475 - +0.9 5,603,485 - -1.1 5,603,556 + +0.9 5,603,556 + +0.6 5,603,557 - +0.7 5,603,560 - +1.2 5,603,560 - -0.4 5,603,585 + -2.6 5,603,585 + -0.4 5,603,586 - +0.9 5,603,586 - +2.1 5,603,586 - +1.2 5,603,593 + -0.8 5,603,593 + +0.2 5,603,593 + -0.4 5,603,593 + +0.7 5,603,593 + +0.3 5,603,593 + +1.1 5,603,594 - -0.0 5,603,594 - +0.4 5,603,594 - +0.7 5,603,594 - -0.9 5,603,597 + -0.9 5,603,598 - +1.2 5,603,622 + +0.4 5,603,623 - -0.8 5,603,639 + -0.3 5,603,639 + +0.1 5,603,639 + +0.4 5,603,639 + -0.6 5,603,640 - -2.9 5,603,640 - -0.3 5,603,640 - -0.7 5,603,640 - -2.6 5,603,653 + +0.3 5,603,671 + +0.1 5,603,671 + +0.8 5,603,700 - +0.7 5,603,738 + +0.0 5,603,785 + Psyr_4725 0.13 +0.2 5,603,785 + Psyr_4725 0.13 -0.4 5,603,785 + Psyr_4725 0.13 +0.7 5,603,785 + Psyr_4725 0.13 -1.1 5,603,785 + Psyr_4725 0.13 -0.1 5,603,785 + Psyr_4725 0.13 -1.7 5,603,785 + Psyr_4725 0.13 +0.7 5,603,786 - Psyr_4725 0.13 +0.1 5,603,786 - Psyr_4725 0.13 -1.4 5,603,786 - Psyr_4725 0.13 -0.4 5,603,786 - Psyr_4725 0.13 -0.8 5,603,786 - Psyr_4725 0.13 +0.8 5,603,919 - Psyr_4725 0.40 +0.9 5,603,959 + Psyr_4725 0.48 -1.0 5,603,959 + Psyr_4725 0.48 +0.7 5,603,959 + Psyr_4725 0.48 -0.6 5,603,959 + Psyr_4725 0.48 -1.2 5,603,959 + Psyr_4725 0.48 +0.4 5,603,960 - Psyr_4725 0.48 -0.0 5,603,960 - Psyr_4725 0.48 +0.6 5,603,960 - Psyr_4725 0.48 -0.0 5,603,960 - Psyr_4725 0.48 -1.1 5,603,960 - Psyr_4725 0.48 -0.2 5,603,960 - Psyr_4725 0.48 -0.7 5,604,091 - Psyr_4725 0.74 -0.5 5,604,091 - Psyr_4725 0.74 -0.2 5,604,091 - Psyr_4725 0.74 -1.2 5,604,197 - +1.6 5,604,198 + +1.0 5,604,199 - -0.4 5,604,232 + +0.1 5,604,232 + +1.2 5,605,137 + Psyr_4726 0.62 +0.7
Or see this region's nucleotide sequence