Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2808 and Psyr_2809 overlap by 4 nucleotides Psyr_2809 and Psyr_2810 overlap by 14 nucleotides Psyr_2810 and Psyr_2811 are separated by 33 nucleotides Psyr_2811 and Psyr_2812 are separated by 395 nucleotides
Psyr_2808: Psyr_2808 - hypothetical protein, at 3,385,197 to 3,385,478
_2808
Psyr_2809: Psyr_2809 - hypothetical protein, at 3,385,475 to 3,386,272
_2809
Psyr_2810: Psyr_2810 - hypothetical protein, at 3,386,259 to 3,387,236
_2810
Psyr_2811: Psyr_2811 - hypothetical protein, at 3,387,270 to 3,387,692
_2811
Psyr_2812: Psyr_2812 - phage repressor protein, Serine peptidase, MEROPS family S24, at 3,388,088 to 3,388,738
_2812
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3385.301 kb on + strand, within Psyr_2808 at 3385.301 kb on + strand, within Psyr_2808 at 3385.302 kb on - strand, within Psyr_2808 at 3385.305 kb on - strand, within Psyr_2808 at 3385.734 kb on + strand, within Psyr_2809 at 3385.735 kb on - strand, within Psyr_2809 at 3385.735 kb on - strand, within Psyr_2809 at 3385.878 kb on + strand, within Psyr_2809 at 3385.879 kb on - strand, within Psyr_2809 at 3385.970 kb on - strand, within Psyr_2809 at 3386.142 kb on + strand, within Psyr_2809 at 3386.266 kb on + strand at 3386.266 kb on + strand at 3386.266 kb on + strand at 3386.267 kb on - strand at 3386.267 kb on - strand at 3386.267 kb on - strand at 3386.407 kb on + strand, within Psyr_2810 at 3386.493 kb on + strand, within Psyr_2810 at 3386.493 kb on + strand at 3386.493 kb on + strand, within Psyr_2810 at 3386.493 kb on + strand, within Psyr_2810 at 3386.581 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.605 kb on + strand, within Psyr_2810 at 3386.606 kb on + strand, within Psyr_2810 at 3386.606 kb on - strand, within Psyr_2810 at 3386.606 kb on - strand, within Psyr_2810 at 3386.606 kb on - strand, within Psyr_2810 at 3386.783 kb on + strand, within Psyr_2810 at 3386.783 kb on + strand, within Psyr_2810 at 3386.784 kb on - strand, within Psyr_2810 at 3386.784 kb on - strand, within Psyr_2810 at 3386.864 kb on + strand, within Psyr_2810 at 3386.864 kb on + strand, within Psyr_2810 at 3386.898 kb on + strand, within Psyr_2810 at 3386.909 kb on - strand, within Psyr_2810 at 3386.975 kb on + strand, within Psyr_2810 at 3387.151 kb on + strand at 3387.229 kb on + strand at 3387.578 kb on + strand, within Psyr_2811 at 3387.578 kb on + strand, within Psyr_2811 at 3387.696 kb on + strand at 3387.792 kb on + strand at 3387.839 kb on + strand at 3387.932 kb on + strand at 3388.073 kb on + strand at 3388.075 kb on + strand at 3388.075 kb on + strand at 3388.083 kb on + strand at 3388.083 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 3,385,301 + Psyr_2808 0.37 -0.5 3,385,301 + Psyr_2808 0.37 -0.8 3,385,302 - Psyr_2808 0.37 -0.7 3,385,305 - Psyr_2808 0.38 +2.6 3,385,734 + Psyr_2809 0.32 -0.2 3,385,735 - Psyr_2809 0.33 -0.4 3,385,735 - Psyr_2809 0.33 +0.1 3,385,878 + Psyr_2809 0.51 -0.8 3,385,879 - Psyr_2809 0.51 +1.3 3,385,970 - Psyr_2809 0.62 +1.5 3,386,142 + Psyr_2809 0.84 +1.8 3,386,266 + -0.2 3,386,266 + +3.1 3,386,266 + +0.4 3,386,267 - -0.4 3,386,267 - +0.7 3,386,267 - -0.2 3,386,407 + Psyr_2810 0.15 -1.2 3,386,493 + Psyr_2810 0.24 -0.6 3,386,493 + -1.0 3,386,493 + Psyr_2810 0.24 -0.4 3,386,493 + Psyr_2810 0.24 -2.1 3,386,581 + Psyr_2810 0.33 -0.4 3,386,605 + Psyr_2810 0.35 -0.4 3,386,605 + Psyr_2810 0.35 -2.3 3,386,605 + Psyr_2810 0.35 -2.0 3,386,605 + Psyr_2810 0.35 -0.7 3,386,606 + Psyr_2810 0.35 +3.2 3,386,606 - Psyr_2810 0.35 +0.0 3,386,606 - Psyr_2810 0.35 +0.3 3,386,606 - Psyr_2810 0.35 +1.3 3,386,783 + Psyr_2810 0.54 -0.7 3,386,783 + Psyr_2810 0.54 +0.3 3,386,784 - Psyr_2810 0.54 -1.2 3,386,784 - Psyr_2810 0.54 -2.3 3,386,864 + Psyr_2810 0.62 +0.3 3,386,864 + Psyr_2810 0.62 +0.9 3,386,898 + Psyr_2810 0.65 +1.4 3,386,909 - Psyr_2810 0.66 -0.5 3,386,975 + Psyr_2810 0.73 +2.6 3,387,151 + +0.0 3,387,229 + +0.1 3,387,578 + Psyr_2811 0.73 -1.1 3,387,578 + Psyr_2811 0.73 +0.3 3,387,696 + -0.7 3,387,792 + +0.2 3,387,839 + +1.0 3,387,932 + +0.0 3,388,073 + +0.0 3,388,075 + -0.2 3,388,075 + -2.1 3,388,083 + -1.0 3,388,083 + -0.1
Or see this region's nucleotide sequence