Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2699 and Psyr_2700 are separated by 421 nucleotides Psyr_2700 and Psyr_2701 are separated by 144 nucleotides
Psyr_2699: Psyr_2699 - conserved hypothetical protein, at 3,278,314 to 3,279,333
_2699
Psyr_2700: Psyr_2700 - PAS, at 3,279,755 to 3,281,359
_2700
Psyr_2701: Psyr_2701 - conserved hypothetical protein, at 3,281,504 to 3,282,646
_2701
Position (kb)
3279
3280
3281
3282 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3279.094 kb on + strand, within Psyr_2699 at 3279.095 kb on - strand, within Psyr_2699 at 3279.095 kb on - strand, within Psyr_2699 at 3279.095 kb on - strand, within Psyr_2699 at 3279.095 kb on - strand, within Psyr_2699 at 3279.095 kb on - strand, within Psyr_2699 at 3279.108 kb on + strand, within Psyr_2699 at 3279.109 kb on - strand, within Psyr_2699 at 3279.162 kb on + strand, within Psyr_2699 at 3279.261 kb on + strand at 3279.333 kb on + strand at 3279.333 kb on + strand at 3279.333 kb on + strand at 3279.334 kb on - strand at 3279.334 kb on - strand at 3279.334 kb on - strand at 3279.339 kb on + strand at 3279.339 kb on + strand at 3279.340 kb on - strand at 3279.361 kb on + strand at 3279.361 kb on + strand at 3279.382 kb on + strand at 3279.382 kb on + strand at 3279.383 kb on - strand at 3279.417 kb on + strand at 3279.418 kb on - strand at 3279.525 kb on - strand at 3279.525 kb on - strand at 3279.602 kb on + strand at 3279.643 kb on + strand at 3279.645 kb on + strand at 3279.645 kb on + strand at 3279.645 kb on + strand at 3279.646 kb on - strand at 3279.674 kb on + strand at 3279.674 kb on + strand at 3279.679 kb on + strand at 3279.680 kb on - strand at 3279.682 kb on + strand at 3279.682 kb on + strand at 3279.682 kb on + strand at 3279.683 kb on - strand at 3279.996 kb on + strand, within Psyr_2700 at 3279.997 kb on - strand, within Psyr_2700 at 3279.997 kb on - strand, within Psyr_2700 at 3280.059 kb on + strand, within Psyr_2700 at 3280.059 kb on + strand, within Psyr_2700 at 3280.060 kb on - strand, within Psyr_2700 at 3280.060 kb on - strand, within Psyr_2700 at 3280.060 kb on - strand, within Psyr_2700 at 3280.060 kb on - strand, within Psyr_2700 at 3280.144 kb on + strand, within Psyr_2700 at 3280.206 kb on + strand, within Psyr_2700 at 3280.206 kb on + strand, within Psyr_2700 at 3280.207 kb on - strand, within Psyr_2700 at 3280.207 kb on - strand, within Psyr_2700 at 3280.240 kb on + strand, within Psyr_2700 at 3280.242 kb on + strand, within Psyr_2700 at 3280.243 kb on - strand, within Psyr_2700 at 3280.247 kb on - strand, within Psyr_2700 at 3280.281 kb on + strand, within Psyr_2700 at 3280.281 kb on + strand, within Psyr_2700 at 3280.281 kb on + strand, within Psyr_2700 at 3280.331 kb on - strand, within Psyr_2700 at 3280.400 kb on - strand, within Psyr_2700 at 3280.407 kb on + strand, within Psyr_2700 at 3280.407 kb on + strand, within Psyr_2700 at 3280.407 kb on + strand, within Psyr_2700 at 3280.407 kb on + strand, within Psyr_2700 at 3280.408 kb on - strand, within Psyr_2700 at 3280.431 kb on + strand, within Psyr_2700 at 3280.438 kb on + strand, within Psyr_2700 at 3280.439 kb on - strand, within Psyr_2700 at 3280.439 kb on - strand, within Psyr_2700 at 3280.439 kb on - strand, within Psyr_2700 at 3280.481 kb on + strand, within Psyr_2700 at 3280.481 kb on + strand, within Psyr_2700 at 3280.481 kb on + strand, within Psyr_2700 at 3280.482 kb on - strand, within Psyr_2700 at 3280.482 kb on - strand, within Psyr_2700 at 3280.567 kb on + strand, within Psyr_2700 at 3280.568 kb on - strand, within Psyr_2700 at 3280.666 kb on + strand, within Psyr_2700 at 3280.732 kb on + strand, within Psyr_2700 at 3280.831 kb on + strand, within Psyr_2700 at 3280.831 kb on + strand, within Psyr_2700 at 3280.831 kb on + strand, within Psyr_2700 at 3280.831 kb on + strand, within Psyr_2700 at 3280.831 kb on + strand, within Psyr_2700 at 3280.832 kb on - strand, within Psyr_2700 at 3280.832 kb on - strand, within Psyr_2700 at 3280.832 kb on - strand, within Psyr_2700 at 3280.978 kb on - strand, within Psyr_2700 at 3280.981 kb on + strand, within Psyr_2700 at 3280.981 kb on + strand, within Psyr_2700 at 3280.981 kb on + strand, within Psyr_2700 at 3280.982 kb on - strand, within Psyr_2700 at 3280.982 kb on - strand, within Psyr_2700 at 3280.982 kb on - strand, within Psyr_2700 at 3280.982 kb on - strand, within Psyr_2700 at 3281.000 kb on - strand, within Psyr_2700 at 3281.002 kb on - strand, within Psyr_2700 at 3281.014 kb on + strand, within Psyr_2700 at 3281.015 kb on - strand, within Psyr_2700 at 3281.015 kb on - strand, within Psyr_2700 at 3281.015 kb on - strand, within Psyr_2700 at 3281.053 kb on - strand, within Psyr_2700 at 3281.293 kb on + strand at 3281.315 kb on - strand at 3281.464 kb on + strand at 3281.464 kb on + strand at 3281.466 kb on + strand at 3281.466 kb on + strand at 3281.467 kb on - strand at 3281.467 kb on - strand at 3281.619 kb on + strand, within Psyr_2701 at 3281.692 kb on + strand at 3281.693 kb on - strand, within Psyr_2701 at 3281.693 kb on - strand, within Psyr_2701 at 3281.693 kb on - strand, within Psyr_2701 at 3281.706 kb on - strand, within Psyr_2701 at 3281.706 kb on - strand, within Psyr_2701 at 3281.745 kb on + strand, within Psyr_2701 at 3281.745 kb on + strand, within Psyr_2701 at 3281.745 kb on + strand, within Psyr_2701 at 3281.745 kb on + strand, within Psyr_2701 at 3281.746 kb on - strand, within Psyr_2701 at 3281.746 kb on - strand, within Psyr_2701 at 3281.872 kb on + strand, within Psyr_2701 at 3281.911 kb on - strand, within Psyr_2701 at 3282.059 kb on + strand, within Psyr_2701 at 3282.059 kb on + strand, within Psyr_2701 at 3282.059 kb on + strand, within Psyr_2701 at 3282.059 kb on + strand, within Psyr_2701 at 3282.060 kb on - strand, within Psyr_2701 at 3282.060 kb on - strand, within Psyr_2701 at 3282.060 kb on - strand, within Psyr_2701 at 3282.060 kb on - strand, within Psyr_2701 at 3282.122 kb on + strand, within Psyr_2701 at 3282.195 kb on + strand, within Psyr_2701 at 3282.195 kb on + strand, within Psyr_2701 at 3282.195 kb on + strand, within Psyr_2701 at 3282.196 kb on - strand, within Psyr_2701 at 3282.196 kb on - strand, within Psyr_2701 at 3282.249 kb on + strand, within Psyr_2701
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 3,279,094 + Psyr_2699 0.76 -1.1 3,279,095 - Psyr_2699 0.77 -0.9 3,279,095 - Psyr_2699 0.77 -0.0 3,279,095 - Psyr_2699 0.77 -0.3 3,279,095 - Psyr_2699 0.77 +2.4 3,279,095 - Psyr_2699 0.77 +0.5 3,279,108 + Psyr_2699 0.78 +1.0 3,279,109 - Psyr_2699 0.78 +1.7 3,279,162 + Psyr_2699 0.83 -0.9 3,279,261 + +1.8 3,279,333 + -2.0 3,279,333 + -1.6 3,279,333 + -1.5 3,279,334 - -1.6 3,279,334 - -1.5 3,279,334 - +0.3 3,279,339 + -1.2 3,279,339 + -2.4 3,279,340 - +0.0 3,279,361 + -0.2 3,279,361 + +0.7 3,279,382 + +0.7 3,279,382 + +0.8 3,279,383 - +0.1 3,279,417 + -0.3 3,279,418 - -0.1 3,279,525 - +1.9 3,279,525 - +0.2 3,279,602 + -0.5 3,279,643 + +0.5 3,279,645 + +0.1 3,279,645 + -0.5 3,279,645 + -1.0 3,279,646 - -0.0 3,279,674 + +0.2 3,279,674 + -1.7 3,279,679 + -0.8 3,279,680 - +0.7 3,279,682 + +0.0 3,279,682 + -0.5 3,279,682 + -0.6 3,279,683 - +1.0 3,279,996 + Psyr_2700 0.15 +1.1 3,279,997 - Psyr_2700 0.15 +0.7 3,279,997 - Psyr_2700 0.15 +0.0 3,280,059 + Psyr_2700 0.19 +1.7 3,280,059 + Psyr_2700 0.19 +0.7 3,280,060 - Psyr_2700 0.19 +0.6 3,280,060 - Psyr_2700 0.19 -0.2 3,280,060 - Psyr_2700 0.19 +0.4 3,280,060 - Psyr_2700 0.19 +0.7 3,280,144 + Psyr_2700 0.24 -0.8 3,280,206 + Psyr_2700 0.28 -0.9 3,280,206 + Psyr_2700 0.28 +1.4 3,280,207 - Psyr_2700 0.28 +0.4 3,280,207 - Psyr_2700 0.28 +0.7 3,280,240 + Psyr_2700 0.30 -0.9 3,280,242 + Psyr_2700 0.30 +0.7 3,280,243 - Psyr_2700 0.30 -0.5 3,280,247 - Psyr_2700 0.31 +1.5 3,280,281 + Psyr_2700 0.33 +0.7 3,280,281 + Psyr_2700 0.33 +0.1 3,280,281 + Psyr_2700 0.33 +0.7 3,280,331 - Psyr_2700 0.36 +0.8 3,280,400 - Psyr_2700 0.40 +0.1 3,280,407 + Psyr_2700 0.41 -1.1 3,280,407 + Psyr_2700 0.41 -2.0 3,280,407 + Psyr_2700 0.41 +0.1 3,280,407 + Psyr_2700 0.41 +2.4 3,280,408 - Psyr_2700 0.41 +1.1 3,280,431 + Psyr_2700 0.42 -0.3 3,280,438 + Psyr_2700 0.43 -0.2 3,280,439 - Psyr_2700 0.43 -0.2 3,280,439 - Psyr_2700 0.43 -0.3 3,280,439 - Psyr_2700 0.43 +1.1 3,280,481 + Psyr_2700 0.45 -1.7 3,280,481 + Psyr_2700 0.45 -0.3 3,280,481 + Psyr_2700 0.45 -0.6 3,280,482 - Psyr_2700 0.45 +0.5 3,280,482 - Psyr_2700 0.45 +0.2 3,280,567 + Psyr_2700 0.51 -1.1 3,280,568 - Psyr_2700 0.51 +1.2 3,280,666 + Psyr_2700 0.57 -0.0 3,280,732 + Psyr_2700 0.61 +0.9 3,280,831 + Psyr_2700 0.67 -0.9 3,280,831 + Psyr_2700 0.67 +0.9 3,280,831 + Psyr_2700 0.67 -1.0 3,280,831 + Psyr_2700 0.67 +0.5 3,280,831 + Psyr_2700 0.67 +0.4 3,280,832 - Psyr_2700 0.67 -0.2 3,280,832 - Psyr_2700 0.67 -0.5 3,280,832 - Psyr_2700 0.67 -1.8 3,280,978 - Psyr_2700 0.76 +0.7 3,280,981 + Psyr_2700 0.76 +1.7 3,280,981 + Psyr_2700 0.76 +0.3 3,280,981 + Psyr_2700 0.76 -0.5 3,280,982 - Psyr_2700 0.76 -0.3 3,280,982 - Psyr_2700 0.76 -0.6 3,280,982 - Psyr_2700 0.76 -1.8 3,280,982 - Psyr_2700 0.76 -0.1 3,281,000 - Psyr_2700 0.78 +0.1 3,281,002 - Psyr_2700 0.78 +2.7 3,281,014 + Psyr_2700 0.78 -0.6 3,281,015 - Psyr_2700 0.79 -0.6 3,281,015 - Psyr_2700 0.79 -0.2 3,281,015 - Psyr_2700 0.79 -0.7 3,281,053 - Psyr_2700 0.81 -1.5 3,281,293 + +0.1 3,281,315 - +0.1 3,281,464 + -0.3 3,281,464 + -1.8 3,281,466 + -2.1 3,281,466 + +0.1 3,281,467 - +0.1 3,281,467 - -2.2 3,281,619 + Psyr_2701 0.10 +0.2 3,281,692 + +0.1 3,281,693 - Psyr_2701 0.17 -2.4 3,281,693 - Psyr_2701 0.17 +0.4 3,281,693 - Psyr_2701 0.17 -1.9 3,281,706 - Psyr_2701 0.18 +1.5 3,281,706 - Psyr_2701 0.18 +0.2 3,281,745 + Psyr_2701 0.21 -1.5 3,281,745 + Psyr_2701 0.21 -0.1 3,281,745 + Psyr_2701 0.21 -0.2 3,281,745 + Psyr_2701 0.21 -1.5 3,281,746 - Psyr_2701 0.21 -1.5 3,281,746 - Psyr_2701 0.21 -2.1 3,281,872 + Psyr_2701 0.32 -0.4 3,281,911 - Psyr_2701 0.36 -0.9 3,282,059 + Psyr_2701 0.49 -1.3 3,282,059 + Psyr_2701 0.49 -0.6 3,282,059 + Psyr_2701 0.49 -1.1 3,282,059 + Psyr_2701 0.49 +0.1 3,282,060 - Psyr_2701 0.49 -2.1 3,282,060 - Psyr_2701 0.49 -0.3 3,282,060 - Psyr_2701 0.49 -0.3 3,282,060 - Psyr_2701 0.49 +0.1 3,282,122 + Psyr_2701 0.54 +2.4 3,282,195 + Psyr_2701 0.60 -0.4 3,282,195 + Psyr_2701 0.60 -0.5 3,282,195 + Psyr_2701 0.60 +1.7 3,282,196 - Psyr_2701 0.61 -0.6 3,282,196 - Psyr_2701 0.61 -0.8 3,282,249 + Psyr_2701 0.65 -0.2
Or see this region's nucleotide sequence