Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2653 and Psyr_2654 are separated by 29 nucleotides Psyr_2654 and Psyr_2655 are separated by 131 nucleotides Psyr_2655 and Psyr_2656 are separated by 171 nucleotides Psyr_2656 and Psyr_2657 are separated by 143 nucleotides
Psyr_2653: Psyr_2653 - ISPsy8, transposase OrfA, at 3,224,311 to 3,224,826
_2653
Psyr_2654: Psyr_2654 - Integrase, catalytic region, at 3,224,856 to 3,225,662
_2654
Psyr_2655: Psyr_2655 - Nitroreductase, at 3,225,794 to 3,226,615
_2655
Psyr_2656: Psyr_2656 - hypothetical protein, at 3,226,787 to 3,227,137
_2656
Psyr_2657: Psyr_2657 - hypothetical protein, at 3,227,281 to 3,228,120
_2657
Position (kb)
3225
3226
3227 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.745 kb on + strand at 3225.746 kb on - strand at 3225.778 kb on + strand at 3225.778 kb on + strand at 3225.779 kb on - strand at 3226.067 kb on + strand, within Psyr_2655 at 3226.067 kb on + strand, within Psyr_2655 at 3226.067 kb on + strand, within Psyr_2655 at 3226.068 kb on - strand, within Psyr_2655 at 3226.096 kb on - strand, within Psyr_2655 at 3226.096 kb on - strand, within Psyr_2655 at 3226.096 kb on - strand, within Psyr_2655 at 3226.114 kb on + strand, within Psyr_2655 at 3226.147 kb on - strand, within Psyr_2655 at 3226.147 kb on - strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.167 kb on - strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.260 kb on - strand, within Psyr_2655 at 3226.347 kb on + strand, within Psyr_2655 at 3226.425 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.727 kb on - strand at 3226.727 kb on - strand at 3226.757 kb on + strand at 3226.758 kb on - strand at 3226.758 kb on - strand at 3226.768 kb on - strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.788 kb on + strand at 3226.789 kb on - strand at 3226.841 kb on - strand, within Psyr_2656 at 3226.841 kb on - strand, within Psyr_2656 at 3226.863 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.939 kb on - strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3227.060 kb on + strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.204 kb on + strand at 3227.204 kb on + strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.377 kb on + strand, within Psyr_2657 at 3227.377 kb on + strand at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.421 kb on - strand, within Psyr_2657 at 3227.451 kb on + strand, within Psyr_2657 at 3227.452 kb on - strand, within Psyr_2657 at 3227.503 kb on + strand, within Psyr_2657 at 3227.504 kb on - strand, within Psyr_2657
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 3,225,718 + +0.2 3,225,718 + -1.3 3,225,718 + +0.7 3,225,718 + +1.1 3,225,718 + -1.9 3,225,718 + +3.4 3,225,718 + -0.3 3,225,718 + +0.4 3,225,718 + +0.9 3,225,719 - -0.6 3,225,719 - -0.2 3,225,719 - +1.9 3,225,719 - +2.0 3,225,719 - +0.3 3,225,719 - +0.9 3,225,745 + -0.0 3,225,746 - +0.0 3,225,778 + +0.7 3,225,778 + -1.9 3,225,779 - +0.4 3,226,067 + Psyr_2655 0.33 +0.7 3,226,067 + Psyr_2655 0.33 -0.2 3,226,067 + Psyr_2655 0.33 +1.1 3,226,068 - Psyr_2655 0.33 +1.7 3,226,096 - Psyr_2655 0.37 -0.1 3,226,096 - Psyr_2655 0.37 +0.9 3,226,096 - Psyr_2655 0.37 -0.7 3,226,114 + Psyr_2655 0.39 -0.6 3,226,147 - Psyr_2655 0.43 +2.7 3,226,147 - Psyr_2655 0.43 +0.4 3,226,166 + Psyr_2655 0.45 -0.3 3,226,166 + Psyr_2655 0.45 -0.4 3,226,166 + Psyr_2655 0.45 -0.8 3,226,166 + Psyr_2655 0.45 +1.8 3,226,166 + Psyr_2655 0.45 +0.7 3,226,166 + Psyr_2655 0.45 +0.2 3,226,166 + Psyr_2655 0.45 -0.3 3,226,166 + Psyr_2655 0.45 +1.7 3,226,167 - Psyr_2655 0.45 +1.4 3,226,259 + Psyr_2655 0.57 +0.1 3,226,259 + Psyr_2655 0.57 +1.9 3,226,259 + Psyr_2655 0.57 +0.6 3,226,259 + Psyr_2655 0.57 +0.7 3,226,259 + Psyr_2655 0.57 -2.0 3,226,260 - Psyr_2655 0.57 +1.0 3,226,347 + Psyr_2655 0.67 -1.5 3,226,425 - Psyr_2655 0.77 -0.8 3,226,459 - Psyr_2655 0.81 +1.5 3,226,459 - Psyr_2655 0.81 +1.2 3,226,460 + Psyr_2655 0.81 -0.9 3,226,460 + Psyr_2655 0.81 -0.9 3,226,460 + Psyr_2655 0.81 +0.7 3,226,460 + Psyr_2655 0.81 -1.3 3,226,461 - Psyr_2655 0.81 +0.5 3,226,461 - Psyr_2655 0.81 -0.5 3,226,701 + -1.1 3,226,701 + +2.0 3,226,701 + +0.0 3,226,701 + -0.1 3,226,701 + -0.7 3,226,701 + -0.3 3,226,701 + -0.9 3,226,701 + -0.1 3,226,702 - -0.0 3,226,702 - -0.4 3,226,702 - +0.1 3,226,702 - -1.3 3,226,702 - +0.5 3,226,727 - -0.3 3,226,727 - +0.7 3,226,757 + +0.2 3,226,758 - -1.1 3,226,758 - +1.4 3,226,768 - -1.8 3,226,771 + +2.1 3,226,771 + -0.2 3,226,771 + +0.6 3,226,772 - +0.1 3,226,772 - -0.2 3,226,772 - +0.7 3,226,772 - +0.1 3,226,772 - -0.6 3,226,772 - -1.9 3,226,788 + +0.4 3,226,789 - +0.5 3,226,841 - Psyr_2656 0.15 +0.8 3,226,841 - Psyr_2656 0.15 -0.5 3,226,863 + Psyr_2656 0.22 -2.0 3,226,938 + Psyr_2656 0.43 -1.8 3,226,938 + Psyr_2656 0.43 -0.8 3,226,939 - Psyr_2656 0.43 +1.7 3,226,942 + Psyr_2656 0.44 -0.3 3,226,942 + Psyr_2656 0.44 -0.3 3,226,942 + Psyr_2656 0.44 +0.1 3,226,942 + Psyr_2656 0.44 +0.7 3,226,942 + Psyr_2656 0.44 +0.7 3,226,942 + Psyr_2656 0.44 -2.2 3,226,943 - Psyr_2656 0.44 +0.1 3,226,943 - Psyr_2656 0.44 +0.3 3,226,943 - Psyr_2656 0.44 +1.1 3,226,943 - Psyr_2656 0.44 -0.2 3,226,943 - Psyr_2656 0.44 +1.3 3,226,943 - Psyr_2656 0.44 -0.5 3,226,946 + Psyr_2656 0.45 -1.1 3,226,946 + Psyr_2656 0.45 +0.5 3,226,946 + Psyr_2656 0.45 -0.6 3,226,946 + Psyr_2656 0.45 -0.9 3,226,947 - Psyr_2656 0.46 +0.0 3,226,947 - Psyr_2656 0.46 +0.6 3,227,060 + Psyr_2656 0.78 -0.1 3,227,061 - Psyr_2656 0.78 -0.2 3,227,061 - Psyr_2656 0.78 +1.0 3,227,149 + +0.4 3,227,149 + -1.3 3,227,149 + +1.3 3,227,149 + +0.2 3,227,149 + -0.2 3,227,150 - +0.7 3,227,150 - -1.6 3,227,150 - -1.5 3,227,150 - +0.9 3,227,150 - -0.1 3,227,150 - -0.1 3,227,150 - -2.5 3,227,204 + +0.5 3,227,204 + +0.0 3,227,205 - +0.1 3,227,205 - -0.8 3,227,205 - -0.3 3,227,306 - -2.6 3,227,306 - -0.3 3,227,306 - -1.5 3,227,377 + Psyr_2657 0.11 +1.1 3,227,377 + +0.1 3,227,378 - Psyr_2657 0.12 -0.8 3,227,378 - Psyr_2657 0.12 +0.5 3,227,378 - Psyr_2657 0.12 -0.9 3,227,378 - Psyr_2657 0.12 +0.7 3,227,378 - Psyr_2657 0.12 +1.1 3,227,378 - Psyr_2657 0.12 -0.1 3,227,421 - Psyr_2657 0.17 +0.6 3,227,451 + Psyr_2657 0.20 -0.9 3,227,452 - Psyr_2657 0.20 -0.5 3,227,503 + Psyr_2657 0.26 +0.7 3,227,504 - Psyr_2657 0.27 -2.3
Or see this region's nucleotide sequence