Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1858 and Psyr_1859 are separated by 110 nucleotides Psyr_1859 and Psyr_1860 are separated by 346 nucleotides Psyr_1860 and Psyr_1861 are separated by 100 nucleotides
Psyr_1858: Psyr_1858 - transcriptional regulator, LuxR family, at 2,135,456 to 2,136,178
_1858
Psyr_1859: Psyr_1859 - conserved hypothetical protein, at 2,136,289 to 2,136,567
_1859
Psyr_1860: Psyr_1860 - Ankyrin, at 2,136,914 to 2,137,459
_1860
Psyr_1861: Psyr_1861 - conserved hypothetical protein, at 2,137,560 to 2,138,078
_1861
Position (kb)
2136
2137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2135.373 kb on + strand at 2135.373 kb on + strand at 2135.382 kb on - strand at 2135.382 kb on - strand at 2135.382 kb on - strand at 2135.493 kb on + strand at 2135.493 kb on + strand at 2135.493 kb on + strand at 2135.493 kb on + strand at 2135.494 kb on - strand at 2135.494 kb on - strand at 2135.494 kb on - strand at 2135.673 kb on - strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.784 kb on - strand, within Psyr_1858 at 2135.828 kb on + strand, within Psyr_1858 at 2135.828 kb on + strand, within Psyr_1858 at 2135.829 kb on - strand, within Psyr_1858 at 2135.834 kb on + strand, within Psyr_1858 at 2135.834 kb on + strand, within Psyr_1858 at 2135.836 kb on + strand, within Psyr_1858 at 2135.878 kb on + strand, within Psyr_1858 at 2135.878 kb on + strand, within Psyr_1858 at 2135.921 kb on + strand, within Psyr_1858 at 2135.921 kb on + strand, within Psyr_1858 at 2136.089 kb on + strand, within Psyr_1858 at 2136.089 kb on + strand, within Psyr_1858 at 2136.089 kb on + strand, within Psyr_1858 at 2136.090 kb on - strand, within Psyr_1858 at 2136.090 kb on - strand, within Psyr_1858 at 2136.107 kb on + strand at 2136.108 kb on - strand at 2136.255 kb on - strand at 2136.302 kb on + strand at 2136.302 kb on + strand at 2136.434 kb on + strand, within Psyr_1859 at 2136.434 kb on + strand, within Psyr_1859 at 2136.435 kb on - strand, within Psyr_1859 at 2136.460 kb on - strand, within Psyr_1859 at 2136.524 kb on + strand, within Psyr_1859 at 2136.559 kb on - strand at 2136.609 kb on + strand at 2136.609 kb on + strand at 2136.610 kb on - strand at 2136.612 kb on + strand at 2136.613 kb on - strand at 2136.613 kb on - strand at 2136.908 kb on + strand at 2136.908 kb on + strand at 2136.908 kb on + strand at 2136.909 kb on - strand at 2136.909 kb on - strand at 2136.912 kb on + strand at 2136.913 kb on - strand at 2136.923 kb on - strand at 2137.097 kb on + strand, within Psyr_1860 at 2137.097 kb on + strand, within Psyr_1860 at 2137.097 kb on + strand, within Psyr_1860 at 2137.097 kb on + strand, within Psyr_1860 at 2137.098 kb on - strand, within Psyr_1860 at 2137.098 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.314 kb on + strand, within Psyr_1860 at 2137.315 kb on - strand, within Psyr_1860 at 2137.493 kb on + strand at 2137.494 kb on - strand at 2137.494 kb on - strand at 2137.494 kb on - strand at 2137.494 kb on - strand at 2137.511 kb on - strand at 2137.511 kb on - strand at 2137.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 2,135,373 + -0.4 2,135,373 + +0.6 2,135,382 - +1.1 2,135,382 - +2.0 2,135,382 - -0.7 2,135,493 + +1.3 2,135,493 + -2.5 2,135,493 + -1.0 2,135,493 + +1.5 2,135,494 - +0.8 2,135,494 - -0.9 2,135,494 - +0.1 2,135,673 - Psyr_1858 0.30 +0.3 2,135,783 + Psyr_1858 0.45 -2.4 2,135,783 + Psyr_1858 0.45 +0.5 2,135,783 + Psyr_1858 0.45 -1.3 2,135,783 + Psyr_1858 0.45 -0.6 2,135,784 - Psyr_1858 0.45 -0.5 2,135,828 + Psyr_1858 0.51 -0.5 2,135,828 + Psyr_1858 0.51 +0.2 2,135,829 - Psyr_1858 0.52 -0.4 2,135,834 + Psyr_1858 0.52 +0.1 2,135,834 + Psyr_1858 0.52 +0.2 2,135,836 + Psyr_1858 0.53 -0.7 2,135,878 + Psyr_1858 0.58 +1.5 2,135,878 + Psyr_1858 0.58 +1.2 2,135,921 + Psyr_1858 0.64 -0.9 2,135,921 + Psyr_1858 0.64 +0.9 2,136,089 + Psyr_1858 0.88 -0.9 2,136,089 + Psyr_1858 0.88 -1.5 2,136,089 + Psyr_1858 0.88 +0.3 2,136,090 - Psyr_1858 0.88 -2.0 2,136,090 - Psyr_1858 0.88 +2.5 2,136,107 + -1.4 2,136,108 - +0.2 2,136,255 - +0.3 2,136,302 + -1.4 2,136,302 + +2.3 2,136,434 + Psyr_1859 0.52 -1.1 2,136,434 + Psyr_1859 0.52 -3.6 2,136,435 - Psyr_1859 0.52 -0.5 2,136,460 - Psyr_1859 0.61 -1.0 2,136,524 + Psyr_1859 0.84 -1.0 2,136,559 - -0.1 2,136,609 + +0.1 2,136,609 + -1.4 2,136,610 - -0.4 2,136,612 + +0.1 2,136,613 - +1.6 2,136,613 - -0.0 2,136,908 + +0.0 2,136,908 + +0.7 2,136,908 + -0.5 2,136,909 - -0.2 2,136,909 - -0.5 2,136,912 + -0.1 2,136,913 - +0.1 2,136,923 - +1.5 2,137,097 + Psyr_1860 0.34 +0.2 2,137,097 + Psyr_1860 0.34 +0.2 2,137,097 + Psyr_1860 0.34 +1.3 2,137,097 + Psyr_1860 0.34 +1.4 2,137,098 - Psyr_1860 0.34 -1.7 2,137,098 - Psyr_1860 0.34 +1.3 2,137,249 - Psyr_1860 0.61 +1.5 2,137,249 - Psyr_1860 0.61 +0.1 2,137,249 - Psyr_1860 0.61 +0.5 2,137,249 - Psyr_1860 0.61 +0.5 2,137,249 - Psyr_1860 0.61 +1.5 2,137,249 - Psyr_1860 0.61 -0.6 2,137,249 - Psyr_1860 0.61 -0.1 2,137,314 + Psyr_1860 0.73 -0.3 2,137,315 - Psyr_1860 0.73 -0.2 2,137,493 + +0.0 2,137,494 - -2.7 2,137,494 - -0.8 2,137,494 - -0.5 2,137,494 - +1.3 2,137,511 - +0.9 2,137,511 - +0.1 2,137,511 - +0.9
Or see this region's nucleotide sequence