Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1421 and Psyr_1422 overlap by 4 nucleotides Psyr_1422 and Psyr_1423 are separated by 428 nucleotides
Psyr_1421: Psyr_1421 - Phage integrase, at 1,607,964 to 1,609,370
_1421
Psyr_1422: Psyr_1422 - Protein of unknown function DUF1528:Relaxase, at 1,609,367 to 1,611,271
_1422
Psyr_1423: Psyr_1423 - Protein of unknown function DUF159, at 1,611,700 to 1,612,392
_1423
Position (kb) 1609
1610
1611
1612 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1608.424 kb on + strand, within Psyr_1421 at 1608.424 kb on + strand, within Psyr_1421 at 1608.424 kb on + strand, within Psyr_1421 at 1608.424 kb on + strand, within Psyr_1421 at 1608.424 kb on + strand, within Psyr_1421 at 1608.762 kb on - strand, within Psyr_1421 at 1608.772 kb on - strand, within Psyr_1421 at 1608.916 kb on + strand, within Psyr_1421 at 1608.959 kb on + strand, within Psyr_1421 at 1609.017 kb on - strand, within Psyr_1421 at 1609.017 kb on - strand, within Psyr_1421 at 1609.133 kb on - strand, within Psyr_1421 at 1609.133 kb on - strand, within Psyr_1421 at 1609.138 kb on + strand, within Psyr_1421 at 1609.138 kb on + strand, within Psyr_1421 at 1609.138 kb on + strand, within Psyr_1421 at 1609.138 kb on + strand, within Psyr_1421 at 1609.138 kb on + strand, within Psyr_1421 at 1609.138 kb on + strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.139 kb on - strand, within Psyr_1421 at 1609.163 kb on - strand, within Psyr_1421 at 1609.163 kb on - strand, within Psyr_1421 at 1609.616 kb on + strand, within Psyr_1422 at 1609.617 kb on - strand, within Psyr_1422 at 1609.617 kb on - strand, within Psyr_1422 at 1609.780 kb on + strand, within Psyr_1422 at 1609.874 kb on - strand, within Psyr_1422 at 1610.026 kb on + strand, within Psyr_1422 at 1610.026 kb on + strand, within Psyr_1422 at 1610.026 kb on + strand, within Psyr_1422 at 1610.372 kb on + strand, within Psyr_1422 at 1610.372 kb on + strand, within Psyr_1422 at 1610.372 kb on + strand, within Psyr_1422 at 1610.372 kb on + strand, within Psyr_1422 at 1610.372 kb on + strand, within Psyr_1422 at 1610.373 kb on - strand, within Psyr_1422 at 1610.373 kb on - strand, within Psyr_1422 at 1610.373 kb on - strand, within Psyr_1422 at 1610.394 kb on - strand, within Psyr_1422 at 1610.521 kb on + strand, within Psyr_1422 at 1610.521 kb on + strand, within Psyr_1422 at 1610.521 kb on + strand, within Psyr_1422 at 1610.522 kb on - strand, within Psyr_1422 at 1610.542 kb on + strand, within Psyr_1422 at 1610.542 kb on + strand, within Psyr_1422 at 1610.542 kb on + strand, within Psyr_1422 at 1610.542 kb on + strand, within Psyr_1422 at 1610.542 kb on + strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.543 kb on - strand, within Psyr_1422 at 1610.631 kb on + strand, within Psyr_1422 at 1610.631 kb on + strand, within Psyr_1422 at 1610.721 kb on + strand, within Psyr_1422 at 1610.721 kb on + strand, within Psyr_1422 at 1610.721 kb on + strand, within Psyr_1422 at 1610.721 kb on + strand, within Psyr_1422 at 1610.722 kb on - strand, within Psyr_1422 at 1610.722 kb on - strand, within Psyr_1422 at 1610.722 kb on - strand, within Psyr_1422 at 1610.722 kb on - strand, within Psyr_1422 at 1610.773 kb on - strand, within Psyr_1422 at 1610.773 kb on - strand, within Psyr_1422 at 1610.773 kb on - strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.961 kb on + strand, within Psyr_1422 at 1610.962 kb on - strand, within Psyr_1422 at 1610.962 kb on - strand, within Psyr_1422 at 1610.962 kb on - strand, within Psyr_1422 at 1610.962 kb on - strand, within Psyr_1422 at 1610.962 kb on - strand, within Psyr_1422 at 1610.973 kb on - strand, within Psyr_1422 at 1610.977 kb on + strand, within Psyr_1422 at 1610.977 kb on + strand, within Psyr_1422 at 1610.977 kb on + strand, within Psyr_1422 at 1610.977 kb on + strand, within Psyr_1422 at 1610.977 kb on + strand, within Psyr_1422 at 1610.978 kb on - strand, within Psyr_1422 at 1610.978 kb on - strand, within Psyr_1422 at 1610.978 kb on - strand, within Psyr_1422 at 1611.027 kb on + strand, within Psyr_1422 at 1611.028 kb on - strand, within Psyr_1422 at 1611.028 kb on - strand, within Psyr_1422 at 1611.028 kb on - strand, within Psyr_1422 at 1611.078 kb on + strand, within Psyr_1422 at 1611.078 kb on + strand, within Psyr_1422 at 1611.078 kb on + strand, within Psyr_1422 at 1611.079 kb on - strand, within Psyr_1422 at 1611.079 kb on - strand, within Psyr_1422 at 1611.099 kb on + strand at 1611.099 kb on + strand at 1611.099 kb on + strand at 1611.099 kb on + strand at 1611.099 kb on + strand at 1611.099 kb on + strand at 1611.265 kb on + strand at 1611.266 kb on - strand at 1611.266 kb on - strand at 1611.266 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.274 kb on - strand at 1611.313 kb on - strand at 1611.313 kb on - strand at 1611.313 kb on - strand at 1611.314 kb on + strand at 1611.314 kb on + strand at 1611.314 kb on + strand at 1611.315 kb on - strand at 1611.315 kb on - strand at 1611.315 kb on - strand at 1611.315 kb on - strand at 1611.315 kb on - strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.341 kb on + strand at 1611.361 kb on + strand at 1611.361 kb on + strand at 1611.362 kb on - strand at 1611.362 kb on - strand at 1611.363 kb on + strand at 1611.363 kb on + strand at 1611.363 kb on + strand at 1611.364 kb on - strand at 1611.364 kb on - strand at 1611.364 kb on - strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.365 kb on + strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.366 kb on - strand at 1611.369 kb on + strand at 1611.369 kb on + strand at 1611.369 kb on + strand at 1611.369 kb on + strand at 1611.369 kb on + strand at 1611.370 kb on - strand at 1611.370 kb on - strand at 1611.370 kb on - strand at 1611.370 kb on - strand at 1611.461 kb on - strand at 1611.563 kb on - strand at 1611.563 kb on - strand at 1611.639 kb on + strand at 1611.639 kb on + strand at 1611.640 kb on - strand at 1611.640 kb on - strand at 1611.640 kb on - strand at 1611.640 kb on - strand at 1611.834 kb on + strand, within Psyr_1423 at 1611.834 kb on + strand, within Psyr_1423 at 1611.834 kb on + strand, within Psyr_1423 at 1611.834 kb on + strand, within Psyr_1423 at 1612.002 kb on + strand, within Psyr_1423 at 1612.002 kb on + strand, within Psyr_1423 at 1612.002 kb on + strand, within Psyr_1423 at 1612.003 kb on - strand, within Psyr_1423 at 1612.003 kb on - strand, within Psyr_1423 at 1612.003 kb on - strand, within Psyr_1423 at 1612.012 kb on - strand, within Psyr_1423 at 1612.012 kb on - strand, within Psyr_1423 at 1612.032 kb on - strand, within Psyr_1423 at 1612.032 kb on - strand, within Psyr_1423
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 1,608,424 + Psyr_1421 0.33 +0.3 1,608,424 + Psyr_1421 0.33 +1.1 1,608,424 + Psyr_1421 0.33 +1.2 1,608,424 + Psyr_1421 0.33 -0.1 1,608,424 + Psyr_1421 0.33 -0.3 1,608,762 - Psyr_1421 0.57 +0.5 1,608,772 - Psyr_1421 0.57 +0.9 1,608,916 + Psyr_1421 0.68 -0.9 1,608,959 + Psyr_1421 0.71 -0.6 1,609,017 - Psyr_1421 0.75 +0.8 1,609,017 - Psyr_1421 0.75 -0.5 1,609,133 - Psyr_1421 0.83 +1.0 1,609,133 - Psyr_1421 0.83 +2.1 1,609,138 + Psyr_1421 0.83 -0.7 1,609,138 + Psyr_1421 0.83 -0.0 1,609,138 + Psyr_1421 0.83 +0.3 1,609,138 + Psyr_1421 0.83 -1.6 1,609,138 + Psyr_1421 0.83 -0.7 1,609,138 + Psyr_1421 0.83 -1.8 1,609,139 - Psyr_1421 0.84 +0.4 1,609,139 - Psyr_1421 0.84 -2.7 1,609,139 - Psyr_1421 0.84 -2.0 1,609,139 - Psyr_1421 0.84 +0.1 1,609,139 - Psyr_1421 0.84 +0.2 1,609,139 - Psyr_1421 0.84 -2.0 1,609,139 - Psyr_1421 0.84 -1.4 1,609,139 - Psyr_1421 0.84 +1.0 1,609,163 - Psyr_1421 0.85 +1.4 1,609,163 - Psyr_1421 0.85 +0.6 1,609,616 + Psyr_1422 0.13 -0.8 1,609,617 - Psyr_1422 0.13 +0.0 1,609,617 - Psyr_1422 0.13 -1.4 1,609,780 + Psyr_1422 0.22 -0.3 1,609,874 - Psyr_1422 0.27 -0.7 1,610,026 + Psyr_1422 0.35 -0.9 1,610,026 + Psyr_1422 0.35 -2.8 1,610,026 + Psyr_1422 0.35 +1.2 1,610,372 + Psyr_1422 0.53 +2.2 1,610,372 + Psyr_1422 0.53 -0.3 1,610,372 + Psyr_1422 0.53 -0.4 1,610,372 + Psyr_1422 0.53 -0.2 1,610,372 + Psyr_1422 0.53 +0.8 1,610,373 - Psyr_1422 0.53 +0.7 1,610,373 - Psyr_1422 0.53 -1.5 1,610,373 - Psyr_1422 0.53 -1.6 1,610,394 - Psyr_1422 0.54 +2.1 1,610,521 + Psyr_1422 0.61 -1.7 1,610,521 + Psyr_1422 0.61 +1.6 1,610,521 + Psyr_1422 0.61 -0.0 1,610,522 - Psyr_1422 0.61 +0.0 1,610,542 + Psyr_1422 0.62 -2.6 1,610,542 + Psyr_1422 0.62 +0.7 1,610,542 + Psyr_1422 0.62 -0.9 1,610,542 + Psyr_1422 0.62 +1.1 1,610,542 + Psyr_1422 0.62 -0.2 1,610,543 - Psyr_1422 0.62 -0.6 1,610,543 - Psyr_1422 0.62 +0.3 1,610,543 - Psyr_1422 0.62 -1.2 1,610,543 - Psyr_1422 0.62 -0.6 1,610,543 - Psyr_1422 0.62 +0.7 1,610,543 - Psyr_1422 0.62 -1.4 1,610,543 - Psyr_1422 0.62 +0.6 1,610,543 - Psyr_1422 0.62 -1.0 1,610,631 + Psyr_1422 0.66 -2.0 1,610,631 + Psyr_1422 0.66 -1.0 1,610,721 + Psyr_1422 0.71 +0.6 1,610,721 + Psyr_1422 0.71 -0.5 1,610,721 + Psyr_1422 0.71 +1.6 1,610,721 + Psyr_1422 0.71 -1.1 1,610,722 - Psyr_1422 0.71 -0.3 1,610,722 - Psyr_1422 0.71 -1.3 1,610,722 - Psyr_1422 0.71 -2.3 1,610,722 - Psyr_1422 0.71 -1.4 1,610,773 - Psyr_1422 0.74 +0.3 1,610,773 - Psyr_1422 0.74 +0.1 1,610,773 - Psyr_1422 0.74 +0.0 1,610,961 + Psyr_1422 0.84 -2.0 1,610,961 + Psyr_1422 0.84 -0.7 1,610,961 + Psyr_1422 0.84 +0.5 1,610,961 + Psyr_1422 0.84 +1.0 1,610,961 + Psyr_1422 0.84 -0.9 1,610,961 + Psyr_1422 0.84 +0.9 1,610,961 + Psyr_1422 0.84 +2.0 1,610,961 + Psyr_1422 0.84 -0.2 1,610,961 + Psyr_1422 0.84 -1.9 1,610,962 - Psyr_1422 0.84 +0.8 1,610,962 - Psyr_1422 0.84 -1.1 1,610,962 - Psyr_1422 0.84 -3.1 1,610,962 - Psyr_1422 0.84 +0.0 1,610,962 - Psyr_1422 0.84 +0.3 1,610,973 - Psyr_1422 0.84 -0.3 1,610,977 + Psyr_1422 0.85 -0.3 1,610,977 + Psyr_1422 0.85 -2.2 1,610,977 + Psyr_1422 0.85 -2.4 1,610,977 + Psyr_1422 0.85 -0.3 1,610,977 + Psyr_1422 0.85 -0.4 1,610,978 - Psyr_1422 0.85 -0.7 1,610,978 - Psyr_1422 0.85 -1.2 1,610,978 - Psyr_1422 0.85 -1.6 1,611,027 + Psyr_1422 0.87 +0.1 1,611,028 - Psyr_1422 0.87 +0.2 1,611,028 - Psyr_1422 0.87 +1.6 1,611,028 - Psyr_1422 0.87 +0.1 1,611,078 + Psyr_1422 0.90 -0.6 1,611,078 + Psyr_1422 0.90 -0.1 1,611,078 + Psyr_1422 0.90 -1.2 1,611,079 - Psyr_1422 0.90 -0.7 1,611,079 - Psyr_1422 0.90 +0.1 1,611,099 + -1.4 1,611,099 + +0.3 1,611,099 + -1.7 1,611,099 + +0.8 1,611,099 + +0.7 1,611,099 + +1.4 1,611,265 + -0.2 1,611,266 - -1.3 1,611,266 - -0.7 1,611,266 - +0.3 1,611,274 - -2.1 1,611,274 - -1.2 1,611,274 - -1.4 1,611,274 - -0.0 1,611,274 - -2.3 1,611,274 - +0.6 1,611,274 - +0.0 1,611,274 - +0.6 1,611,313 - +0.1 1,611,313 - -0.3 1,611,313 - -1.0 1,611,314 + -1.5 1,611,314 + +0.3 1,611,314 + -0.4 1,611,315 - -0.8 1,611,315 - +0.6 1,611,315 - -1.4 1,611,315 - -1.7 1,611,315 - -0.6 1,611,341 + -1.0 1,611,341 + +1.6 1,611,341 + -0.4 1,611,341 + +0.0 1,611,341 + -0.7 1,611,341 + -0.7 1,611,341 + -1.9 1,611,341 + +1.6 1,611,341 + +1.3 1,611,341 + -0.8 1,611,341 + -1.2 1,611,341 + -0.3 1,611,341 + -0.8 1,611,341 + -2.3 1,611,361 + -1.6 1,611,361 + -0.6 1,611,362 - -0.6 1,611,362 - +0.6 1,611,363 + +3.6 1,611,363 + +1.6 1,611,363 + -2.5 1,611,364 - +0.3 1,611,364 - -1.9 1,611,364 - -0.4 1,611,365 + +0.6 1,611,365 + +1.6 1,611,365 + -1.8 1,611,365 + -0.3 1,611,365 + -0.6 1,611,365 + +0.0 1,611,365 + -1.9 1,611,365 + +0.2 1,611,365 + -1.6 1,611,365 + -0.4 1,611,365 + +0.0 1,611,365 + +0.5 1,611,365 + +0.3 1,611,365 + +0.0 1,611,365 + +1.2 1,611,365 + +0.6 1,611,365 + -2.3 1,611,365 + -0.2 1,611,365 + -2.0 1,611,365 + -1.2 1,611,365 + +0.0 1,611,365 + +0.0 1,611,365 + -1.6 1,611,366 - -0.4 1,611,366 - +0.6 1,611,366 - +1.0 1,611,366 - -2.2 1,611,366 - -1.7 1,611,366 - -0.7 1,611,366 - +0.7 1,611,366 - +1.2 1,611,366 - -2.0 1,611,366 - -0.4 1,611,366 - +1.1 1,611,366 - -0.9 1,611,366 - -2.2 1,611,366 - +0.8 1,611,366 - -1.4 1,611,366 - +0.0 1,611,366 - +0.0 1,611,366 - -1.3 1,611,366 - -2.6 1,611,366 - +1.6 1,611,366 - -1.9 1,611,366 - +1.6 1,611,369 + -1.2 1,611,369 + -2.1 1,611,369 + -1.4 1,611,369 + +0.6 1,611,369 + -1.1 1,611,370 - +0.0 1,611,370 - +0.7 1,611,370 - -0.4 1,611,370 - -1.6 1,611,461 - -0.6 1,611,563 - -2.9 1,611,563 - +0.4 1,611,639 + +0.9 1,611,639 + -0.3 1,611,640 - -0.4 1,611,640 - -1.2 1,611,640 - +0.4 1,611,640 - +0.1 1,611,834 + Psyr_1423 0.19 -0.2 1,611,834 + Psyr_1423 0.19 +0.4 1,611,834 + Psyr_1423 0.19 -1.0 1,611,834 + Psyr_1423 0.19 +0.6 1,612,002 + Psyr_1423 0.44 +1.8 1,612,002 + Psyr_1423 0.44 -0.9 1,612,002 + Psyr_1423 0.44 -0.6 1,612,003 - Psyr_1423 0.44 -2.2 1,612,003 - Psyr_1423 0.44 +0.3 1,612,003 - Psyr_1423 0.44 +1.2 1,612,012 - Psyr_1423 0.45 -0.8 1,612,012 - Psyr_1423 0.45 +0.6 1,612,032 - Psyr_1423 0.48 -1.9 1,612,032 - Psyr_1423 0.48 +1.6
Or see this region's nucleotide sequence