Experiment: KB with Anisodamine Hydrobromide () 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0682 and Psyr_0683 are separated by 120 nucleotides Psyr_0683 and Psyr_0684 are separated by 96 nucleotides Psyr_0684 and Psyr_0685 overlap by 14 nucleotides
Psyr_0682: Psyr_0682 - DNA helicase/exodeoxyribonuclease V, alpha subunit, at 784,699 to 786,792
_0682
Psyr_0683: Psyr_0683 - NUDIX hydrolase, at 786,913 to 787,317
_0683
Psyr_0684: Psyr_0684 - hypothetical protein, at 787,414 to 787,830
_0684
Psyr_0685: Psyr_0685 - PAAR, at 787,817 to 788,956
_0685
Position (kb) 787
788 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 786.836 kb on + strand at 786.836 kb on + strand at 786.837 kb on - strand at 786.837 kb on - strand at 786.837 kb on - strand at 786.854 kb on - strand at 786.860 kb on + strand at 786.906 kb on + strand at 786.906 kb on + strand at 786.906 kb on + strand at 786.907 kb on - strand at 787.062 kb on - strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.134 kb on + strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.135 kb on - strand, within Psyr_0683 at 787.273 kb on - strand, within Psyr_0683 at 787.273 kb on - strand, within Psyr_0683 at 787.273 kb on - strand, within Psyr_0683 at 787.311 kb on + strand at 787.311 kb on + strand at 787.312 kb on - strand at 787.343 kb on + strand at 787.343 kb on + strand at 787.343 kb on + strand at 787.344 kb on - strand at 787.344 kb on - strand at 787.344 kb on - strand at 787.344 kb on - strand at 787.344 kb on - strand at 787.369 kb on + strand at 787.370 kb on - strand at 787.370 kb on - strand at 787.370 kb on - strand at 787.498 kb on - strand, within Psyr_0684 at 787.514 kb on + strand, within Psyr_0684 at 787.514 kb on + strand, within Psyr_0684 at 787.514 kb on + strand, within Psyr_0684 at 787.515 kb on - strand, within Psyr_0684 at 787.515 kb on - strand, within Psyr_0684 at 787.515 kb on - strand, within Psyr_0684 at 787.515 kb on - strand, within Psyr_0684 at 787.515 kb on - strand, within Psyr_0684 at 787.573 kb on - strand, within Psyr_0684 at 787.597 kb on - strand, within Psyr_0684 at 787.620 kb on + strand, within Psyr_0684 at 787.620 kb on + strand, within Psyr_0684 at 787.620 kb on + strand, within Psyr_0684 at 787.621 kb on - strand, within Psyr_0684 at 787.621 kb on - strand, within Psyr_0684 at 787.621 kb on - strand, within Psyr_0684 at 787.621 kb on - strand, within Psyr_0684 at 787.621 kb on - strand, within Psyr_0684 at 787.691 kb on - strand, within Psyr_0684 at 787.691 kb on - strand, within Psyr_0684 at 787.694 kb on + strand, within Psyr_0684 at 787.695 kb on - strand, within Psyr_0684 at 787.725 kb on - strand, within Psyr_0684 at 787.727 kb on + strand, within Psyr_0684 at 787.727 kb on + strand, within Psyr_0684 at 787.727 kb on + strand, within Psyr_0684 at 787.727 kb on + strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.728 kb on - strand, within Psyr_0684 at 787.750 kb on + strand, within Psyr_0684 at 787.750 kb on + strand, within Psyr_0684 at 787.751 kb on - strand, within Psyr_0684 at 787.751 kb on - strand, within Psyr_0684 at 787.751 kb on - strand, within Psyr_0684 at 787.751 kb on - strand, within Psyr_0684 at 787.751 kb on - strand, within Psyr_0684 at 787.754 kb on + strand, within Psyr_0684 at 787.754 kb on + strand, within Psyr_0684 at 787.754 kb on + strand, within Psyr_0684 at 787.754 kb on + strand, within Psyr_0684 at 787.755 kb on - strand, within Psyr_0684 at 787.786 kb on + strand, within Psyr_0684 at 787.786 kb on + strand, within Psyr_0684 at 787.786 kb on + strand, within Psyr_0684 at 787.786 kb on + strand, within Psyr_0684 at 787.787 kb on - strand, within Psyr_0684 at 787.787 kb on - strand, within Psyr_0684 at 787.787 kb on - strand, within Psyr_0684 at 787.787 kb on - strand, within Psyr_0684 at 787.789 kb on - strand at 787.789 kb on - strand at 787.806 kb on - strand at 787.860 kb on - strand at 787.860 kb on - strand at 787.861 kb on + strand at 787.861 kb on + strand at 787.861 kb on + strand at 787.861 kb on + strand at 787.861 kb on + strand at 787.861 kb on + strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.862 kb on - strand at 787.865 kb on + strand at 787.865 kb on + strand at 787.926 kb on + strand at 787.927 kb on - strand at 788.036 kb on + strand, within Psyr_0685 at 788.036 kb on + strand, within Psyr_0685 at 788.037 kb on - strand, within Psyr_0685 at 788.037 kb on - strand, within Psyr_0685 at 788.037 kb on - strand, within Psyr_0685 at 788.107 kb on + strand, within Psyr_0685 at 788.108 kb on - strand, within Psyr_0685 at 788.125 kb on - strand, within Psyr_0685 at 788.149 kb on + strand, within Psyr_0685 at 788.150 kb on - strand, within Psyr_0685 at 788.150 kb on - strand, within Psyr_0685 at 788.155 kb on - strand, within Psyr_0685 at 788.155 kb on - strand, within Psyr_0685 at 788.155 kb on - strand, within Psyr_0685 at 788.160 kb on + strand, within Psyr_0685 at 788.161 kb on - strand, within Psyr_0685 at 788.161 kb on - strand, within Psyr_0685 at 788.161 kb on - strand, within Psyr_0685 at 788.210 kb on + strand, within Psyr_0685 at 788.294 kb on - strand, within Psyr_0685 at 788.346 kb on + strand, within Psyr_0685 at 788.346 kb on + strand, within Psyr_0685 at 788.346 kb on + strand, within Psyr_0685 at 788.346 kb on + strand, within Psyr_0685 at 788.347 kb on - strand, within Psyr_0685 at 788.347 kb on - strand, within Psyr_0685 at 788.347 kb on - strand, within Psyr_0685 at 788.350 kb on + strand, within Psyr_0685 at 788.351 kb on - strand, within Psyr_0685 at 788.351 kb on - strand, within Psyr_0685 at 788.390 kb on + strand, within Psyr_0685 at 788.390 kb on + strand, within Psyr_0685 at 788.390 kb on + strand, within Psyr_0685 at 788.391 kb on - strand, within Psyr_0685 at 788.391 kb on - strand, within Psyr_0685 at 788.391 kb on - strand, within Psyr_0685 at 788.396 kb on + strand, within Psyr_0685 at 788.397 kb on - strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.440 kb on + strand, within Psyr_0685 at 788.441 kb on - strand, within Psyr_0685 at 788.441 kb on - strand, within Psyr_0685 at 788.441 kb on - strand, within Psyr_0685 at 788.441 kb on - strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.442 kb on + strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.443 kb on - strand, within Psyr_0685 at 788.544 kb on - strand, within Psyr_0685 at 788.544 kb on - strand, within Psyr_0685 at 788.544 kb on - strand, within Psyr_0685 at 788.544 kb on - strand, within Psyr_0685
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Anisodamine Hydrobromide () 10 mM remove 786,836 + -0.9 786,836 + -0.1 786,837 - -0.2 786,837 - +0.2 786,837 - +0.1 786,854 - -2.1 786,860 + -0.0 786,906 + -0.5 786,906 + +1.3 786,906 + +0.8 786,907 - +0.4 787,062 - Psyr_0683 0.37 +1.3 787,134 + Psyr_0683 0.55 -3.3 787,134 + Psyr_0683 0.55 -0.1 787,134 + Psyr_0683 0.55 +1.5 787,134 + Psyr_0683 0.55 -0.6 787,134 + Psyr_0683 0.55 +1.1 787,134 + Psyr_0683 0.55 -0.5 787,134 + Psyr_0683 0.55 +1.0 787,134 + Psyr_0683 0.55 +0.8 787,134 + Psyr_0683 0.55 -1.1 787,134 + Psyr_0683 0.55 -1.9 787,134 + Psyr_0683 0.55 -0.6 787,134 + Psyr_0683 0.55 +0.1 787,134 + Psyr_0683 0.55 -0.5 787,134 + Psyr_0683 0.55 -0.3 787,134 + Psyr_0683 0.55 +0.5 787,134 + Psyr_0683 0.55 +0.9 787,134 + Psyr_0683 0.55 +0.6 787,134 + Psyr_0683 0.55 +0.3 787,134 + Psyr_0683 0.55 +1.0 787,135 - Psyr_0683 0.55 -0.1 787,135 - Psyr_0683 0.55 +0.1 787,135 - Psyr_0683 0.55 -0.8 787,135 - Psyr_0683 0.55 +0.6 787,135 - Psyr_0683 0.55 +0.2 787,135 - Psyr_0683 0.55 +2.0 787,135 - Psyr_0683 0.55 +1.0 787,135 - Psyr_0683 0.55 +0.8 787,135 - Psyr_0683 0.55 +0.1 787,135 - Psyr_0683 0.55 -1.9 787,135 - Psyr_0683 0.55 +0.6 787,135 - Psyr_0683 0.55 -0.1 787,135 - Psyr_0683 0.55 +1.8 787,135 - Psyr_0683 0.55 -0.5 787,135 - Psyr_0683 0.55 -1.0 787,135 - Psyr_0683 0.55 +0.2 787,135 - Psyr_0683 0.55 -0.6 787,135 - Psyr_0683 0.55 -1.3 787,273 - Psyr_0683 0.89 -2.0 787,273 - Psyr_0683 0.89 +0.7 787,273 - Psyr_0683 0.89 -0.0 787,311 + -1.3 787,311 + +0.1 787,312 - -1.3 787,343 + -2.1 787,343 + -0.9 787,343 + +2.1 787,344 - -0.7 787,344 - -1.1 787,344 - +0.8 787,344 - +0.3 787,344 - +2.0 787,369 + -1.7 787,370 - +0.7 787,370 - -0.7 787,370 - +1.3 787,498 - Psyr_0684 0.20 -0.2 787,514 + Psyr_0684 0.24 -1.3 787,514 + Psyr_0684 0.24 -1.1 787,514 + Psyr_0684 0.24 -1.4 787,515 - Psyr_0684 0.24 +0.5 787,515 - Psyr_0684 0.24 +0.1 787,515 - Psyr_0684 0.24 +0.8 787,515 - Psyr_0684 0.24 -1.2 787,515 - Psyr_0684 0.24 -0.7 787,573 - Psyr_0684 0.38 +0.6 787,597 - Psyr_0684 0.44 -1.6 787,620 + Psyr_0684 0.49 -2.3 787,620 + Psyr_0684 0.49 -1.7 787,620 + Psyr_0684 0.49 -0.7 787,621 - Psyr_0684 0.50 -0.4 787,621 - Psyr_0684 0.50 -0.7 787,621 - Psyr_0684 0.50 -0.8 787,621 - Psyr_0684 0.50 +0.1 787,621 - Psyr_0684 0.50 -0.0 787,691 - Psyr_0684 0.66 +2.2 787,691 - Psyr_0684 0.66 -0.9 787,694 + Psyr_0684 0.67 -1.1 787,695 - Psyr_0684 0.67 +2.5 787,725 - Psyr_0684 0.75 -2.9 787,727 + Psyr_0684 0.75 +1.5 787,727 + Psyr_0684 0.75 +1.5 787,727 + Psyr_0684 0.75 -1.1 787,727 + Psyr_0684 0.75 -0.5 787,728 - Psyr_0684 0.75 -0.1 787,728 - Psyr_0684 0.75 -0.5 787,728 - Psyr_0684 0.75 -1.8 787,728 - Psyr_0684 0.75 +0.5 787,728 - Psyr_0684 0.75 -0.5 787,728 - Psyr_0684 0.75 -0.4 787,728 - Psyr_0684 0.75 -1.0 787,750 + Psyr_0684 0.81 -1.5 787,750 + Psyr_0684 0.81 -2.2 787,751 - Psyr_0684 0.81 -0.3 787,751 - Psyr_0684 0.81 +0.4 787,751 - Psyr_0684 0.81 -0.0 787,751 - Psyr_0684 0.81 +0.8 787,751 - Psyr_0684 0.81 -0.5 787,754 + Psyr_0684 0.82 -1.1 787,754 + Psyr_0684 0.82 +1.5 787,754 + Psyr_0684 0.82 -1.5 787,754 + Psyr_0684 0.82 -0.1 787,755 - Psyr_0684 0.82 +0.1 787,786 + Psyr_0684 0.89 -1.9 787,786 + Psyr_0684 0.89 -0.1 787,786 + Psyr_0684 0.89 -0.1 787,786 + Psyr_0684 0.89 -0.5 787,787 - Psyr_0684 0.89 +0.3 787,787 - Psyr_0684 0.89 +0.1 787,787 - Psyr_0684 0.89 +0.5 787,787 - Psyr_0684 0.89 +1.5 787,789 - -0.1 787,789 - +0.7 787,806 - +0.5 787,860 - -0.3 787,860 - +0.1 787,861 + -2.8 787,861 + -0.1 787,861 + -1.3 787,861 + -1.9 787,861 + -1.7 787,861 + +1.5 787,862 - -0.8 787,862 - -1.3 787,862 - -1.1 787,862 - -2.4 787,862 - -0.7 787,862 - -0.9 787,862 - -1.9 787,862 - -0.5 787,862 - -0.8 787,865 + +0.8 787,865 + +1.5 787,926 + -0.1 787,927 - +0.9 788,036 + Psyr_0685 0.19 -1.5 788,036 + Psyr_0685 0.19 -2.0 788,037 - Psyr_0685 0.19 -0.1 788,037 - Psyr_0685 0.19 +0.2 788,037 - Psyr_0685 0.19 -0.5 788,107 + Psyr_0685 0.25 -0.5 788,108 - Psyr_0685 0.26 +1.1 788,125 - Psyr_0685 0.27 -1.9 788,149 + Psyr_0685 0.29 -0.5 788,150 - Psyr_0685 0.29 -0.0 788,150 - Psyr_0685 0.29 -0.0 788,155 - Psyr_0685 0.30 -1.5 788,155 - Psyr_0685 0.30 +1.5 788,155 - Psyr_0685 0.30 +0.7 788,160 + Psyr_0685 0.30 +0.5 788,161 - Psyr_0685 0.30 +2.3 788,161 - Psyr_0685 0.30 -1.9 788,161 - Psyr_0685 0.30 +0.4 788,210 + Psyr_0685 0.34 +0.1 788,294 - Psyr_0685 0.42 -2.6 788,346 + Psyr_0685 0.46 -0.1 788,346 + Psyr_0685 0.46 +0.1 788,346 + Psyr_0685 0.46 +1.2 788,346 + Psyr_0685 0.46 -0.9 788,347 - Psyr_0685 0.46 -0.1 788,347 - Psyr_0685 0.46 -0.7 788,347 - Psyr_0685 0.46 +0.0 788,350 + Psyr_0685 0.47 +0.5 788,351 - Psyr_0685 0.47 +0.2 788,351 - Psyr_0685 0.47 -2.1 788,390 + Psyr_0685 0.50 +0.5 788,390 + Psyr_0685 0.50 -0.5 788,390 + Psyr_0685 0.50 -0.4 788,391 - Psyr_0685 0.50 -0.7 788,391 - Psyr_0685 0.50 -0.3 788,391 - Psyr_0685 0.50 -0.8 788,396 + Psyr_0685 0.51 +0.1 788,397 - Psyr_0685 0.51 -0.9 788,440 + Psyr_0685 0.55 +0.3 788,440 + Psyr_0685 0.55 +1.1 788,440 + Psyr_0685 0.55 +0.2 788,440 + Psyr_0685 0.55 -0.5 788,440 + Psyr_0685 0.55 -1.5 788,440 + Psyr_0685 0.55 +2.1 788,440 + Psyr_0685 0.55 -1.9 788,440 + Psyr_0685 0.55 +2.0 788,440 + Psyr_0685 0.55 -0.4 788,441 - Psyr_0685 0.55 -1.7 788,441 - Psyr_0685 0.55 +1.5 788,441 - Psyr_0685 0.55 -0.1 788,441 - Psyr_0685 0.55 -1.5 788,442 + Psyr_0685 0.55 +0.7 788,442 + Psyr_0685 0.55 -0.4 788,442 + Psyr_0685 0.55 -0.1 788,442 + Psyr_0685 0.55 +0.2 788,442 + Psyr_0685 0.55 +0.8 788,442 + Psyr_0685 0.55 +0.2 788,442 + Psyr_0685 0.55 -0.1 788,442 + Psyr_0685 0.55 +1.5 788,442 + Psyr_0685 0.55 +0.4 788,442 + Psyr_0685 0.55 -2.1 788,443 - Psyr_0685 0.55 -0.6 788,443 - Psyr_0685 0.55 -0.5 788,443 - Psyr_0685 0.55 +0.2 788,443 - Psyr_0685 0.55 +0.4 788,443 - Psyr_0685 0.55 +1.1 788,443 - Psyr_0685 0.55 -0.9 788,443 - Psyr_0685 0.55 +1.2 788,443 - Psyr_0685 0.55 +1.3 788,443 - Psyr_0685 0.55 +1.9 788,443 - Psyr_0685 0.55 +0.1 788,443 - Psyr_0685 0.55 -0.5 788,443 - Psyr_0685 0.55 +0.5 788,544 - Psyr_0685 0.64 -0.5 788,544 - Psyr_0685 0.64 +0.9 788,544 - Psyr_0685 0.64 -1.1 788,544 - Psyr_0685 0.64 +0.5
Or see this region's nucleotide sequence