Experiment: Mixed culture; Synechococcus str. WH7803 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_458 and HP15_459 are separated by 46 nucleotides HP15_459 and HP15_460 are separated by 184 nucleotides HP15_460 and HP15_461 are separated by 8 nucleotides
HP15_458: HP15_458 - transcriptional regulator, LysR family protein, at 464,016 to 464,906
_458
HP15_459: HP15_459 - glycine cleavage H-protein, subgroup, at 464,953 to 465,345
_459
HP15_460: HP15_460 - SAM-dependent methyltransferase, at 465,530 to 466,735
_460
HP15_461: HP15_461 - Histidinol-phosphatase (EC:3.1.3.15) <small>(from data)</small>, at 466,744 to 467,400
_461
Position (kb) 465
466
467 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 464.541 kb on - strand, within HP15_458 at 464.552 kb on + strand, within HP15_458 at 464.552 kb on + strand, within HP15_458 at 464.552 kb on + strand, within HP15_458 at 464.662 kb on - strand, within HP15_458 at 464.677 kb on - strand, within HP15_458 at 464.677 kb on - strand, within HP15_458 at 464.758 kb on - strand, within HP15_458 at 464.764 kb on - strand, within HP15_458 at 464.800 kb on - strand, within HP15_458 at 464.845 kb on + strand at 464.900 kb on + strand at 464.921 kb on - strand at 465.233 kb on - strand, within HP15_459 at 465.233 kb on - strand, within HP15_459 at 465.436 kb on - strand at 465.612 kb on + strand at 465.703 kb on - strand, within HP15_460 at 465.703 kb on - strand, within HP15_460 at 465.715 kb on - strand, within HP15_460 at 465.743 kb on - strand, within HP15_460 at 465.758 kb on - strand, within HP15_460 at 465.758 kb on - strand, within HP15_460 at 465.944 kb on - strand, within HP15_460 at 465.966 kb on + strand, within HP15_460 at 465.971 kb on + strand, within HP15_460 at 466.424 kb on - strand, within HP15_460 at 466.488 kb on - strand, within HP15_460 at 466.489 kb on - strand, within HP15_460 at 466.500 kb on + strand, within HP15_460 at 466.513 kb on - strand, within HP15_460 at 467.084 kb on + strand, within HP15_461 at 467.448 kb on + strand at 467.456 kb on - strand at 467.572 kb on - strand at 467.590 kb on + strand at 467.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH7803 10:1 starting OD to HP15 remove 464,541 - HP15_458 0.59 -0.2 464,552 + HP15_458 0.60 -2.0 464,552 + HP15_458 0.60 -2.9 464,552 + HP15_458 0.60 -2.5 464,662 - HP15_458 0.73 -2.4 464,677 - HP15_458 0.74 -1.8 464,677 - HP15_458 0.74 -2.0 464,758 - HP15_458 0.83 -1.2 464,764 - HP15_458 0.84 -0.9 464,800 - HP15_458 0.88 -1.5 464,845 + -0.5 464,900 + +0.5 464,921 - +1.1 465,233 - HP15_459 0.71 -1.1 465,233 - HP15_459 0.71 -1.1 465,436 - -0.7 465,612 + +1.9 465,703 - HP15_460 0.14 -0.3 465,703 - HP15_460 0.14 +0.8 465,715 - HP15_460 0.15 +2.1 465,743 - HP15_460 0.18 +0.1 465,758 - HP15_460 0.19 +0.9 465,758 - HP15_460 0.19 +1.0 465,944 - HP15_460 0.34 -2.0 465,966 + HP15_460 0.36 +1.1 465,971 + HP15_460 0.37 +0.4 466,424 - HP15_460 0.74 +1.4 466,488 - HP15_460 0.79 -1.2 466,489 - HP15_460 0.80 +0.3 466,500 + HP15_460 0.80 +0.2 466,513 - HP15_460 0.82 -0.3 467,084 + HP15_461 0.52 -2.9 467,448 + -0.2 467,456 - -0.4 467,572 - +2.1 467,590 + +1.0 467,598 - +1.7
Or see this region's nucleotide sequence