Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19815 and BWI76_RS19820 are separated by 137 nucleotides BWI76_RS19820 and BWI76_RS19825 are separated by 61 nucleotides BWI76_RS19825 and BWI76_RS19830 are separated by 80 nucleotides
BWI76_RS19815: BWI76_RS19815 - ATP-dependent helicase, at 4,143,952 to 4,145,709
_RS19815
BWI76_RS19820: BWI76_RS19820 - 50S ribosomal protein L25, at 4,145,847 to 4,146,131
_RS19820
BWI76_RS19825: BWI76_RS19825 - DNA mismatch repair protein MutT, at 4,146,193 to 4,146,786
_RS19825
BWI76_RS19830: BWI76_RS19830 - DeoR family transcriptional regulator, at 4,146,867 to 4,147,625
_RS19830
Position (kb)
4145
4146
4147 Strain fitness (log2 ratio)
-1
0
1 at 4144.989 kb on + strand, within BWI76_RS19815 at 4145.306 kb on + strand, within BWI76_RS19815 at 4145.481 kb on + strand, within BWI76_RS19815 at 4145.494 kb on - strand, within BWI76_RS19815 at 4145.504 kb on + strand, within BWI76_RS19815 at 4145.504 kb on + strand, within BWI76_RS19815 at 4145.523 kb on - strand, within BWI76_RS19815 at 4145.534 kb on + strand at 4146.196 kb on + strand at 4146.557 kb on + strand, within BWI76_RS19825 at 4146.665 kb on - strand, within BWI76_RS19825 at 4146.691 kb on + strand, within BWI76_RS19825
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,144,989 + BWI76_RS19815 0.59 +0.2 4,145,306 + BWI76_RS19815 0.77 -0.2 4,145,481 + BWI76_RS19815 0.87 +0.5 4,145,494 - BWI76_RS19815 0.88 -0.2 4,145,504 + BWI76_RS19815 0.88 -0.0 4,145,504 + BWI76_RS19815 0.88 +0.0 4,145,523 - BWI76_RS19815 0.89 -0.1 4,145,534 + -0.2 4,146,196 + -0.4 4,146,557 + BWI76_RS19825 0.61 -0.7 4,146,665 - BWI76_RS19825 0.79 +0.0 4,146,691 + BWI76_RS19825 0.84 -0.3
Or see this region's nucleotide sequence