Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14945 and BWI76_RS14950 are separated by 4 nucleotides BWI76_RS14950 and BWI76_RS14955 are separated by 3 nucleotides
BWI76_RS14945: BWI76_RS14945 - PTS system, cellobiose-specific IIC component, at 3,131,958 to 3,133,277
_RS14945
BWI76_RS14950: BWI76_RS14950 - glycosylasparaginase, at 3,133,282 to 3,134,235
_RS14950
BWI76_RS14955: BWI76_RS14955 - peptidase M17 leucyl aminopeptidase domain-containing protein, at 3,134,239 to 3,135,594
_RS14955
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-1
0
1 at 3132.620 kb on + strand, within BWI76_RS14945 at 3132.657 kb on + strand, within BWI76_RS14945 at 3133.014 kb on + strand, within BWI76_RS14945 at 3133.024 kb on - strand, within BWI76_RS14945 at 3133.074 kb on - strand, within BWI76_RS14945 at 3133.131 kb on + strand, within BWI76_RS14945 at 3133.370 kb on + strand at 3133.421 kb on - strand at 3133.637 kb on + strand, within BWI76_RS14950 at 3133.851 kb on - strand, within BWI76_RS14950 at 3133.851 kb on - strand, within BWI76_RS14950 at 3134.204 kb on - strand at 3134.232 kb on - strand at 3134.529 kb on - strand, within BWI76_RS14955 at 3134.561 kb on + strand, within BWI76_RS14955 at 3135.083 kb on + strand, within BWI76_RS14955 at 3135.123 kb on + strand, within BWI76_RS14955
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 3,132,620 + BWI76_RS14945 0.50 +0.1 3,132,657 + BWI76_RS14945 0.53 +0.0 3,133,014 + BWI76_RS14945 0.80 +0.1 3,133,024 - BWI76_RS14945 0.81 +0.5 3,133,074 - BWI76_RS14945 0.85 +0.1 3,133,131 + BWI76_RS14945 0.89 -0.2 3,133,370 + -0.4 3,133,421 - +0.1 3,133,637 + BWI76_RS14950 0.37 -1.0 3,133,851 - BWI76_RS14950 0.60 -1.1 3,133,851 - BWI76_RS14950 0.60 -0.8 3,134,204 - -0.0 3,134,232 - +0.7 3,134,529 - BWI76_RS14955 0.21 +0.0 3,134,561 + BWI76_RS14955 0.24 +0.1 3,135,083 + BWI76_RS14955 0.62 +0.1 3,135,123 + BWI76_RS14955 0.65 +1.4
Or see this region's nucleotide sequence