Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14820 and BWI76_RS14825 are separated by 210 nucleotides BWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotides BWI76_RS14830 and BWI76_RS14835 are separated by 133 nucleotides
BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522
_RS14820
BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332
_RS14825
BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904
_RS14830
BWI76_RS14835: BWI76_RS14835 - hypothetical protein, at 3,108,038 to 3,108,430
_RS14835
Position (kb)
3106
3107
3108 Strain fitness (log2 ratio)
-2
-1
0
1 at 3106.369 kb on + strand, within BWI76_RS14820 at 3106.660 kb on - strand at 3106.674 kb on - strand at 3106.846 kb on - strand, within BWI76_RS14825 at 3106.983 kb on + strand, within BWI76_RS14825 at 3107.604 kb on - strand, within BWI76_RS14830 at 3107.727 kb on - strand, within BWI76_RS14830 at 3107.735 kb on + strand, within BWI76_RS14830 at 3107.821 kb on + strand, within BWI76_RS14830 at 3108.022 kb on + strand at 3108.130 kb on - strand, within BWI76_RS14835
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 3,106,369 + BWI76_RS14820 0.83 +0.1 3,106,660 - -1.9 3,106,674 - -0.2 3,106,846 - BWI76_RS14825 0.19 -0.7 3,106,983 + BWI76_RS14825 0.42 -0.9 3,107,604 - BWI76_RS14830 0.47 +0.4 3,107,727 - BWI76_RS14830 0.69 +0.5 3,107,735 + BWI76_RS14830 0.70 +0.8 3,107,821 + BWI76_RS14830 0.85 -0.4 3,108,022 + -0.3 3,108,130 - BWI76_RS14835 0.23 -0.1
Or see this region's nucleotide sequence