Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14505 and BWI76_RS14510 are separated by 27 nucleotides BWI76_RS14510 and BWI76_RS14515 overlap by 8 nucleotides
BWI76_RS14505: BWI76_RS14505 - salicylate synthase Irp9, at 3,028,508 to 3,029,818
_RS14505
BWI76_RS14510: BWI76_RS14510 - MFS transporter, at 3,029,846 to 3,031,126
_RS14510
BWI76_RS14515: BWI76_RS14515 - yersiniabactin-iron ABC transporter permease ATP-binding protein YbtQ, at 3,031,119 to 3,032,921
_RS14515
Position (kb)
3029
3030
3031
3032 Strain fitness (log2 ratio)
-2
-1
0
1 at 3028.983 kb on - strand, within BWI76_RS14505 at 3029.349 kb on - strand, within BWI76_RS14505 at 3029.734 kb on - strand at 3030.343 kb on - strand, within BWI76_RS14510 at 3030.519 kb on + strand, within BWI76_RS14510 at 3030.530 kb on + strand, within BWI76_RS14510 at 3030.653 kb on + strand, within BWI76_RS14510 at 3030.661 kb on - strand, within BWI76_RS14510 at 3030.934 kb on - strand, within BWI76_RS14510 at 3031.050 kb on - strand at 3031.217 kb on - strand at 3031.242 kb on - strand at 3031.512 kb on + strand, within BWI76_RS14515 at 3031.573 kb on - strand, within BWI76_RS14515 at 3032.035 kb on + strand, within BWI76_RS14515
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 3,028,983 - BWI76_RS14505 0.36 +1.0 3,029,349 - BWI76_RS14505 0.64 +0.5 3,029,734 - -0.1 3,030,343 - BWI76_RS14510 0.39 -0.5 3,030,519 + BWI76_RS14510 0.53 -1.1 3,030,530 + BWI76_RS14510 0.53 -0.8 3,030,653 + BWI76_RS14510 0.63 -0.4 3,030,661 - BWI76_RS14510 0.64 -1.2 3,030,934 - BWI76_RS14510 0.85 -0.8 3,031,050 - -1.8 3,031,217 - -0.1 3,031,242 - +0.9 3,031,512 + BWI76_RS14515 0.22 -1.1 3,031,573 - BWI76_RS14515 0.25 -0.6 3,032,035 + BWI76_RS14515 0.51 -1.1
Or see this region's nucleotide sequence