Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12695 and BWI76_RS12700 are separated by 277 nucleotides BWI76_RS12700 and BWI76_RS12705 overlap by 50 nucleotides
BWI76_RS12695: BWI76_RS12695 - zinc transporter ZntB, at 2,646,758 to 2,647,741
_RS12695
BWI76_RS12700: BWI76_RS12700 - hypothetical protein, at 2,648,019 to 2,648,273
_RS12700
BWI76_RS12705: BWI76_RS12705 - ATP-dependent RNA helicase, at 2,648,224 to 2,649,597
_RS12705
Position (kb)
2648
2649 Strain fitness (log2 ratio)
-1
0
1 at 2647.035 kb on - strand, within BWI76_RS12695 at 2647.127 kb on + strand, within BWI76_RS12695 at 2647.398 kb on + strand, within BWI76_RS12695 at 2647.475 kb on - strand, within BWI76_RS12695 at 2647.581 kb on + strand, within BWI76_RS12695 at 2647.706 kb on + strand at 2647.840 kb on + strand at 2647.864 kb on + strand at 2647.872 kb on - strand at 2648.068 kb on + strand, within BWI76_RS12700 at 2648.076 kb on - strand, within BWI76_RS12700 at 2648.396 kb on + strand, within BWI76_RS12705 at 2648.424 kb on - strand, within BWI76_RS12705 at 2648.438 kb on - strand, within BWI76_RS12705 at 2648.438 kb on - strand, within BWI76_RS12705 at 2648.662 kb on + strand, within BWI76_RS12705 at 2648.673 kb on + strand, within BWI76_RS12705 at 2648.815 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 2,647,035 - BWI76_RS12695 0.28 -0.9 2,647,127 + BWI76_RS12695 0.38 +0.6 2,647,398 + BWI76_RS12695 0.65 +0.3 2,647,475 - BWI76_RS12695 0.73 -1.2 2,647,581 + BWI76_RS12695 0.84 +0.4 2,647,706 + -1.0 2,647,840 + +0.2 2,647,864 + +0.1 2,647,872 - -0.6 2,648,068 + BWI76_RS12700 0.19 +0.4 2,648,076 - BWI76_RS12700 0.22 -0.1 2,648,396 + BWI76_RS12705 0.13 +0.5 2,648,424 - BWI76_RS12705 0.15 +0.4 2,648,438 - BWI76_RS12705 0.16 -0.6 2,648,438 - BWI76_RS12705 0.16 -1.0 2,648,662 + BWI76_RS12705 0.32 +0.3 2,648,673 + BWI76_RS12705 0.33 +0.2 2,648,815 + BWI76_RS12705 0.43 -0.2 2,649,044 + BWI76_RS12705 0.60 -0.2 2,649,044 + BWI76_RS12705 0.60 +0.9
Or see this region's nucleotide sequence