Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10960 and BWI76_RS10965 are separated by 209 nucleotides BWI76_RS10965 and BWI76_RS10970 overlap by 15 nucleotides BWI76_RS10970 and BWI76_RS10975 are separated by 73 nucleotides
BWI76_RS10960: BWI76_RS10960 - lipid A biosynthesis lauroyl acyltransferase, at 2,313,862 to 2,314,782
_RS10960
BWI76_RS10965: BWI76_RS10965 - sulfurtransferase, at 2,314,992 to 2,316,056
_RS10965
BWI76_RS10970: BWI76_RS10970 - DUF2770 domain-containing protein, at 2,316,042 to 2,316,155
_RS10970
BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347
_RS10975
Position (kb)
2314
2315
2316
2317 Strain fitness (log2 ratio)
-1
0
1
2 at 2314.790 kb on - strand at 2314.790 kb on - strand at 2314.790 kb on - strand at 2314.931 kb on - strand at 2314.984 kb on + strand at 2314.992 kb on - strand at 2315.034 kb on - strand at 2315.050 kb on + strand at 2315.050 kb on + strand at 2315.058 kb on - strand at 2315.121 kb on + strand at 2315.138 kb on + strand, within BWI76_RS10965 at 2315.151 kb on + strand, within BWI76_RS10965 at 2315.205 kb on + strand, within BWI76_RS10965 at 2315.306 kb on + strand, within BWI76_RS10965 at 2315.320 kb on + strand, within BWI76_RS10965 at 2315.909 kb on - strand, within BWI76_RS10965 at 2316.539 kb on - strand, within BWI76_RS10975 at 2316.610 kb on - strand, within BWI76_RS10975
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 2,314,790 - +0.0 2,314,790 - +0.0 2,314,790 - +0.9 2,314,931 - -0.4 2,314,984 + +0.6 2,314,992 - +0.1 2,315,034 - -1.4 2,315,050 + -0.1 2,315,050 + +0.0 2,315,058 - +1.0 2,315,121 + +2.2 2,315,138 + BWI76_RS10965 0.14 -1.5 2,315,151 + BWI76_RS10965 0.15 +0.1 2,315,205 + BWI76_RS10965 0.20 -0.8 2,315,306 + BWI76_RS10965 0.29 -0.6 2,315,320 + BWI76_RS10965 0.31 -1.3 2,315,909 - BWI76_RS10965 0.86 -0.3 2,316,539 - BWI76_RS10975 0.28 +0.5 2,316,610 - BWI76_RS10975 0.34 +1.2
Or see this region's nucleotide sequence