Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02525 and BWI76_RS02530 overlap by 8 nucleotides BWI76_RS02530 and BWI76_RS02535 are separated by 92 nucleotides BWI76_RS02535 and BWI76_RS02540 are separated by 75 nucleotides
BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921
_RS02525
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
BWI76_RS02535: BWI76_RS02535 - RNA-binding protein Hfq, at 519,957 to 520,265
_RS02535
BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621
_RS02540
Position (kb) 518
519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 517.926 kb on - strand, within BWI76_RS02525 at 518.074 kb on - strand, within BWI76_RS02525 at 518.152 kb on + strand, within BWI76_RS02525 at 518.287 kb on + strand, within BWI76_RS02525 at 518.325 kb on + strand, within BWI76_RS02525 at 518.468 kb on - strand, within BWI76_RS02525 at 518.502 kb on + strand, within BWI76_RS02525 at 518.512 kb on + strand, within BWI76_RS02525 at 518.621 kb on - strand, within BWI76_RS02525 at 518.651 kb on + strand, within BWI76_RS02525 at 518.652 kb on - strand, within BWI76_RS02525 at 518.659 kb on - strand, within BWI76_RS02525 at 518.758 kb on + strand at 518.816 kb on + strand at 518.846 kb on + strand at 518.854 kb on - strand at 518.854 kb on - strand at 518.940 kb on + strand at 519.180 kb on + strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.305 kb on - strand, within BWI76_RS02530 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand at 519.922 kb on + strand at 520.239 kb on + strand at 520.333 kb on + strand at 520.365 kb on - strand at 520.427 kb on - strand at 520.528 kb on - strand, within BWI76_RS02540 at 520.794 kb on - strand, within BWI76_RS02540 at 520.799 kb on - strand, within BWI76_RS02540 at 520.825 kb on - strand, within BWI76_RS02540 at 520.849 kb on + strand, within BWI76_RS02540
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 517,926 - BWI76_RS02525 0.47 -0.4 518,074 - BWI76_RS02525 0.55 +0.2 518,152 + BWI76_RS02525 0.59 +0.5 518,287 + BWI76_RS02525 0.67 -0.8 518,325 + BWI76_RS02525 0.69 +0.4 518,468 - BWI76_RS02525 0.76 +1.0 518,502 + BWI76_RS02525 0.78 +0.7 518,512 + BWI76_RS02525 0.78 -0.4 518,621 - BWI76_RS02525 0.84 +0.2 518,651 + BWI76_RS02525 0.86 -0.0 518,652 - BWI76_RS02525 0.86 -0.8 518,659 - BWI76_RS02525 0.86 -1.5 518,758 + -0.5 518,816 + -0.4 518,846 + -0.1 518,854 - -1.6 518,854 - -0.5 518,940 + +1.8 519,180 + BWI76_RS02530 0.28 +0.9 519,287 - BWI76_RS02530 0.39 -1.4 519,287 - BWI76_RS02530 0.39 -2.0 519,305 - BWI76_RS02530 0.41 -1.4 519,530 + BWI76_RS02530 0.65 -0.0 519,776 + -1.3 519,853 + -0.0 519,882 + +0.9 519,922 + +0.2 520,239 + -3.6 520,333 + +1.6 520,365 - -1.1 520,427 - -0.2 520,528 - BWI76_RS02540 0.15 +0.6 520,794 - BWI76_RS02540 0.35 +0.3 520,799 - BWI76_RS02540 0.36 -0.1 520,825 - BWI76_RS02540 0.38 -0.5 520,849 + BWI76_RS02540 0.40 -1.1
Or see this region's nucleotide sequence