Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00750 and BWI76_RS00755 are separated by 9 nucleotides BWI76_RS00755 and BWI76_RS00760 are separated by 91 nucleotides BWI76_RS00760 and BWI76_RS00765 are separated by 91 nucleotides
BWI76_RS00750: BWI76_RS00750 - ATP-dependent protease ATP-binding subunit HslU, at 152,271 to 153,605
_RS00750
BWI76_RS00755: BWI76_RS00755 - ATP-dependent protease subunit HslV, at 153,615 to 154,145
_RS00755
BWI76_RS00760: BWI76_RS00760 - cell division protein FtsN, at 154,237 to 155,211
_RS00760
BWI76_RS00765: BWI76_RS00765 - DNA-binding transcriptional regulator CytR, at 155,303 to 156,331
_RS00765
Position (kb) 154
155
156 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 153.484 kb on - strand at 153.489 kb on - strand at 153.823 kb on + strand, within BWI76_RS00755 at 153.967 kb on + strand, within BWI76_RS00755 at 154.069 kb on + strand, within BWI76_RS00755 at 154.077 kb on - strand, within BWI76_RS00755 at 154.082 kb on - strand, within BWI76_RS00755 at 154.114 kb on - strand at 154.369 kb on - strand, within BWI76_RS00760 at 154.417 kb on - strand, within BWI76_RS00760 at 154.532 kb on + strand, within BWI76_RS00760 at 154.540 kb on - strand, within BWI76_RS00760 at 154.540 kb on - strand, within BWI76_RS00760 at 154.540 kb on - strand, within BWI76_RS00760 at 154.565 kb on - strand, within BWI76_RS00760 at 155.382 kb on - strand at 155.397 kb on + strand at 155.521 kb on + strand, within BWI76_RS00765 at 155.521 kb on + strand, within BWI76_RS00765 at 155.973 kb on - strand, within BWI76_RS00765 at 156.005 kb on + strand, within BWI76_RS00765 at 156.005 kb on + strand, within BWI76_RS00765 at 156.005 kb on + strand, within BWI76_RS00765 at 156.041 kb on + strand, within BWI76_RS00765 at 156.059 kb on + strand, within BWI76_RS00765
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 153,484 - -0.8 153,489 - +0.9 153,823 + BWI76_RS00755 0.39 +0.5 153,967 + BWI76_RS00755 0.66 +0.1 154,069 + BWI76_RS00755 0.85 +0.3 154,077 - BWI76_RS00755 0.87 -0.7 154,082 - BWI76_RS00755 0.88 +0.0 154,114 - +0.5 154,369 - BWI76_RS00760 0.14 -1.5 154,417 - BWI76_RS00760 0.18 -0.1 154,532 + BWI76_RS00760 0.30 -0.8 154,540 - BWI76_RS00760 0.31 -0.7 154,540 - BWI76_RS00760 0.31 -0.4 154,540 - BWI76_RS00760 0.31 -0.2 154,565 - BWI76_RS00760 0.34 -3.2 155,382 - +1.0 155,397 + +1.2 155,521 + BWI76_RS00765 0.21 -0.0 155,521 + BWI76_RS00765 0.21 -0.1 155,973 - BWI76_RS00765 0.65 +1.0 156,005 + BWI76_RS00765 0.68 +0.9 156,005 + BWI76_RS00765 0.68 +0.4 156,005 + BWI76_RS00765 0.68 +0.8 156,041 + BWI76_RS00765 0.72 +1.0 156,059 + BWI76_RS00765 0.73 -0.6
Or see this region's nucleotide sequence