Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4510 and BT4511 are separated by 135 nucleotides BT4511 and BT4512 are separated by 177 nucleotides BT4512 and BT4513 are separated by 4 nucleotides BT4513 and BT4514 are separated by 18 nucleotides
BT4510: BT4510 - putative helicase (NCBI ptt file), at 5,926,125 to 5,928,080
BT4510
BT4511: BT4511 - hypothetical protein (NCBI ptt file), at 5,928,216 to 5,928,650
BT4511
BT4512: BT4512 - putative zinc-type alcohol dehydrogenase (NCBI ptt file), at 5,928,828 to 5,929,880
BT4512
BT4513: BT4513 - putative rRNA methylase (NCBI ptt file), at 5,929,885 to 5,930,685
BT4513
BT4514: BT4514 - hypothetical protein (NCBI ptt file), at 5,930,704 to 5,931,522
BT4514
Position (kb)
5928
5929
5930 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5927.943 kb on - strand at 5927.955 kb on - strand at 5927.955 kb on - strand at 5928.002 kb on - strand at 5928.035 kb on - strand at 5928.051 kb on + strand at 5928.052 kb on - strand at 5928.052 kb on - strand at 5928.094 kb on + strand at 5928.094 kb on + strand at 5928.222 kb on + strand at 5928.223 kb on - strand at 5928.314 kb on + strand, within BT4511 at 5928.361 kb on + strand, within BT4511 at 5928.362 kb on - strand, within BT4511 at 5928.403 kb on + strand, within BT4511 at 5928.442 kb on - strand, within BT4511 at 5928.524 kb on - strand, within BT4511 at 5928.545 kb on - strand, within BT4511 at 5928.670 kb on + strand at 5928.745 kb on - strand at 5928.750 kb on - strand at 5928.838 kb on - strand at 5928.843 kb on + strand at 5928.844 kb on - strand at 5928.844 kb on - strand at 5928.936 kb on - strand, within BT4512 at 5928.941 kb on + strand, within BT4512 at 5928.956 kb on + strand, within BT4512 at 5928.956 kb on + strand, within BT4512 at 5928.968 kb on + strand, within BT4512 at 5929.016 kb on - strand, within BT4512 at 5929.016 kb on - strand, within BT4512 at 5929.016 kb on - strand, within BT4512 at 5929.046 kb on + strand, within BT4512 at 5929.046 kb on + strand, within BT4512 at 5929.047 kb on - strand, within BT4512 at 5929.126 kb on - strand, within BT4512 at 5929.166 kb on - strand, within BT4512 at 5929.207 kb on + strand, within BT4512 at 5929.207 kb on + strand, within BT4512 at 5929.207 kb on + strand, within BT4512 at 5929.235 kb on + strand, within BT4512 at 5929.335 kb on - strand, within BT4512 at 5929.409 kb on + strand, within BT4512 at 5929.410 kb on - strand, within BT4512 at 5929.472 kb on - strand, within BT4512 at 5929.665 kb on + strand, within BT4512 at 5929.666 kb on - strand, within BT4512 at 5929.700 kb on - strand, within BT4512 at 5929.755 kb on - strand, within BT4512 at 5929.767 kb on - strand, within BT4512 at 5929.806 kb on + strand at 5929.807 kb on - strand at 5929.874 kb on + strand at 5929.875 kb on - strand at 5929.884 kb on + strand at 5929.885 kb on - strand at 5929.932 kb on - strand at 5929.968 kb on + strand, within BT4513 at 5930.048 kb on + strand, within BT4513 at 5930.068 kb on - strand, within BT4513 at 5930.126 kb on - strand, within BT4513 at 5930.152 kb on - strand, within BT4513 at 5930.164 kb on + strand, within BT4513 at 5930.274 kb on + strand, within BT4513 at 5930.274 kb on + strand, within BT4513 at 5930.334 kb on - strand, within BT4513 at 5930.343 kb on + strand, within BT4513 at 5930.344 kb on - strand, within BT4513 at 5930.594 kb on - strand, within BT4513 at 5930.597 kb on + strand, within BT4513 at 5930.610 kb on + strand at 5930.693 kb on - strand at 5930.751 kb on + strand at 5930.753 kb on + strand at 5930.831 kb on - strand, within BT4514 at 5930.841 kb on + strand, within BT4514 at 5930.851 kb on - strand, within BT4514
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 5,927,943 - -1.1 5,927,955 - -0.5 5,927,955 - +0.1 5,928,002 - +0.1 5,928,035 - -1.6 5,928,051 + +0.5 5,928,052 - -0.6 5,928,052 - -0.9 5,928,094 + -1.3 5,928,094 + -0.9 5,928,222 + +1.4 5,928,223 - -0.9 5,928,314 + BT4511 0.23 -3.9 5,928,361 + BT4511 0.33 -1.5 5,928,362 - BT4511 0.34 +0.1 5,928,403 + BT4511 0.43 +0.6 5,928,442 - BT4511 0.52 -2.3 5,928,524 - BT4511 0.71 -1.9 5,928,545 - BT4511 0.76 -1.9 5,928,670 + -0.9 5,928,745 - -0.9 5,928,750 - +0.1 5,928,838 - -1.5 5,928,843 + +0.4 5,928,844 - +1.1 5,928,844 - +1.6 5,928,936 - BT4512 0.10 -2.0 5,928,941 + BT4512 0.11 -1.9 5,928,956 + BT4512 0.12 -2.7 5,928,956 + BT4512 0.12 -1.3 5,928,968 + BT4512 0.13 -1.9 5,929,016 - BT4512 0.18 -1.4 5,929,016 - BT4512 0.18 -0.9 5,929,016 - BT4512 0.18 -1.1 5,929,046 + BT4512 0.21 +0.1 5,929,046 + BT4512 0.21 -0.9 5,929,047 - BT4512 0.21 -2.5 5,929,126 - BT4512 0.28 -1.5 5,929,166 - BT4512 0.32 -1.5 5,929,207 + BT4512 0.36 -1.6 5,929,207 + BT4512 0.36 +0.8 5,929,207 + BT4512 0.36 -0.9 5,929,235 + BT4512 0.39 +2.1 5,929,335 - BT4512 0.48 +1.6 5,929,409 + BT4512 0.55 -3.6 5,929,410 - BT4512 0.55 -1.9 5,929,472 - BT4512 0.61 +0.6 5,929,665 + BT4512 0.79 +3.1 5,929,666 - BT4512 0.80 +0.8 5,929,700 - BT4512 0.83 +0.1 5,929,755 - BT4512 0.88 -1.5 5,929,767 - BT4512 0.89 +2.4 5,929,806 + +2.6 5,929,807 - -2.4 5,929,874 + -1.9 5,929,875 - -0.8 5,929,884 + -0.9 5,929,885 - -2.5 5,929,932 - -1.4 5,929,968 + BT4513 0.10 +0.1 5,930,048 + BT4513 0.20 -1.5 5,930,068 - BT4513 0.23 -1.4 5,930,126 - BT4513 0.30 -0.7 5,930,152 - BT4513 0.33 -1.5 5,930,164 + BT4513 0.35 -2.4 5,930,274 + BT4513 0.49 +2.4 5,930,274 + BT4513 0.49 -3.3 5,930,334 - BT4513 0.56 +0.1 5,930,343 + BT4513 0.57 -1.2 5,930,344 - BT4513 0.57 -2.5 5,930,594 - BT4513 0.89 -0.4 5,930,597 + BT4513 0.89 -0.9 5,930,610 + -0.9 5,930,693 - +1.1 5,930,751 + +0.1 5,930,753 + -0.9 5,930,831 - BT4514 0.16 -0.9 5,930,841 + BT4514 0.17 -0.9 5,930,851 - BT4514 0.18 -1.9
Or see this region's nucleotide sequence