Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28825 and RR42_RS28830 are separated by 7 nucleotides RR42_RS28830 and RR42_RS28835 are separated by 269 nucleotides RR42_RS28835 and RR42_RS28840 are separated by 116 nucleotides RR42_RS28840 and RR42_RS28845 are separated by 40 nucleotides
RR42_RS28825: RR42_RS28825 - energy transducer TonB, at 1,819,720 to 1,822,122
_RS28825
RR42_RS28830: RR42_RS28830 - hypothetical protein, at 1,822,130 to 1,822,462
_RS28830
RR42_RS28835: RR42_RS28835 - gluconate:H+ symporter (gntT) (from data) , at 1,822,732 to 1,824,093
_RS28835
RR42_RS28840: RR42_RS28840 - hypothetical protein, at 1,824,210 to 1,824,602
_RS28840
RR42_RS28845: RR42_RS28845 - D-aminoacylase, at 1,824,643 to 1,826,136
_RS28845
Position (kb)
1822
1823
1824
1825 Strain fitness (log2 ratio)
-2
-1
0
1 at 1821.892 kb on + strand at 1821.907 kb on + strand at 1821.908 kb on - strand at 1821.978 kb on + strand at 1822.131 kb on + strand at 1822.131 kb on + strand at 1822.132 kb on - strand at 1822.221 kb on - strand, within RR42_RS28830 at 1822.278 kb on + strand, within RR42_RS28830 at 1822.279 kb on - strand, within RR42_RS28830 at 1822.279 kb on - strand, within RR42_RS28830 at 1822.456 kb on + strand at 1822.457 kb on - strand at 1822.494 kb on - strand at 1822.528 kb on - strand at 1822.631 kb on + strand at 1822.632 kb on - strand at 1822.739 kb on - strand at 1822.838 kb on + strand at 1822.839 kb on - strand at 1823.228 kb on + strand, within RR42_RS28835 at 1823.228 kb on + strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.511 kb on + strand, within RR42_RS28835 at 1823.511 kb on + strand, within RR42_RS28835 at 1823.574 kb on - strand, within RR42_RS28835 at 1823.574 kb on - strand, within RR42_RS28835 at 1823.755 kb on - strand, within RR42_RS28835 at 1823.755 kb on - strand, within RR42_RS28835 at 1823.911 kb on + strand, within RR42_RS28835 at 1823.911 kb on + strand, within RR42_RS28835 at 1823.995 kb on - strand at 1824.059 kb on + strand at 1824.059 kb on + strand at 1824.229 kb on + strand at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.509 kb on - strand, within RR42_RS28840 at 1824.596 kb on + strand at 1824.636 kb on + strand at 1824.636 kb on + strand at 1824.636 kb on + strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.644 kb on + strand at 1824.645 kb on - strand at 1824.848 kb on + strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 1,821,892 + -0.6 1,821,907 + -2.0 1,821,908 - -0.2 1,821,978 + -0.9 1,822,131 + -0.4 1,822,131 + -0.4 1,822,132 - +1.0 1,822,221 - RR42_RS28830 0.27 -0.2 1,822,278 + RR42_RS28830 0.44 +0.6 1,822,279 - RR42_RS28830 0.45 -1.4 1,822,279 - RR42_RS28830 0.45 -0.2 1,822,456 + -1.6 1,822,457 - -0.6 1,822,494 - -1.3 1,822,528 - -0.6 1,822,631 + -1.0 1,822,632 - -0.2 1,822,739 - +0.5 1,822,838 + +0.1 1,822,839 - -0.8 1,823,228 + RR42_RS28835 0.36 -0.8 1,823,228 + RR42_RS28835 0.36 +0.5 1,823,229 - RR42_RS28835 0.36 -0.9 1,823,229 - RR42_RS28835 0.36 +0.4 1,823,229 - RR42_RS28835 0.36 -1.0 1,823,229 - RR42_RS28835 0.36 -0.3 1,823,229 - RR42_RS28835 0.36 -0.3 1,823,511 + RR42_RS28835 0.57 -0.5 1,823,511 + RR42_RS28835 0.57 +0.6 1,823,574 - RR42_RS28835 0.62 -0.5 1,823,574 - RR42_RS28835 0.62 -0.8 1,823,755 - RR42_RS28835 0.75 -0.8 1,823,755 - RR42_RS28835 0.75 -0.6 1,823,911 + RR42_RS28835 0.87 +0.2 1,823,911 + RR42_RS28835 0.87 -1.4 1,823,995 - +0.4 1,824,059 + +0.7 1,824,059 + -0.3 1,824,229 + -0.2 1,824,508 + RR42_RS28840 0.76 -0.3 1,824,508 + RR42_RS28840 0.76 -1.0 1,824,508 + RR42_RS28840 0.76 -0.3 1,824,508 + RR42_RS28840 0.76 -0.3 1,824,509 - RR42_RS28840 0.76 +0.3 1,824,596 + +0.3 1,824,636 + +0.3 1,824,636 + -0.6 1,824,636 + +0.5 1,824,637 - -0.5 1,824,637 - -0.5 1,824,637 - -0.6 1,824,637 - -0.6 1,824,637 - -0.6 1,824,637 - -0.6 1,824,644 + +0.2 1,824,645 - +0.0 1,824,848 + RR42_RS28845 0.14 +0.6 1,825,092 - RR42_RS28845 0.30 +1.1 1,825,092 - RR42_RS28845 0.30 +0.4 1,825,092 - RR42_RS28845 0.30 +0.5
Or see this region's nucleotide sequence