Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23535 and RR42_RS23540 are separated by 30 nucleotides RR42_RS23540 and RR42_RS23545 are separated by 134 nucleotides RR42_RS23545 and RR42_RS23550 are separated by 26 nucleotides RR42_RS23550 and RR42_RS23555 overlap by 8 nucleotides
RR42_RS23535: RR42_RS23535 - hypothetical protein, at 606,107 to 607,333
_RS23535
RR42_RS23540: RR42_RS23540 - alpha-ketoglutarate-dependent dioxygenase, at 607,364 to 608,017
_RS23540
RR42_RS23545: RR42_RS23545 - hypothetical protein, at 608,152 to 609,594
_RS23545
RR42_RS23550: RR42_RS23550 - flagellar export protein FliJ, at 609,621 to 610,076
_RS23550
RR42_RS23555: RR42_RS23555 - ATP synthase, at 610,069 to 611,532
_RS23555
Position (kb) 608
609
610 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 607.165 kb on - strand, within RR42_RS23535 at 607.274 kb on + strand at 607.365 kb on + strand at 607.476 kb on - strand, within RR42_RS23540 at 607.900 kb on + strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 607.901 kb on - strand, within RR42_RS23540 at 608.081 kb on + strand at 608.081 kb on + strand at 608.082 kb on - strand at 608.082 kb on - strand at 608.082 kb on - strand at 608.106 kb on - strand at 608.819 kb on + strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 608.820 kb on - strand, within RR42_RS23545 at 609.075 kb on + strand, within RR42_RS23545 at 609.148 kb on - strand, within RR42_RS23545 at 609.334 kb on + strand, within RR42_RS23545 at 609.335 kb on - strand, within RR42_RS23545 at 609.585 kb on + strand at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.667 kb on + strand, within RR42_RS23550 at 609.668 kb on - strand, within RR42_RS23550 at 609.732 kb on - strand, within RR42_RS23550 at 609.732 kb on - strand, within RR42_RS23550 at 610.052 kb on + strand at 610.053 kb on - strand at 610.070 kb on + strand at 610.070 kb on + strand at 610.070 kb on + strand at 610.098 kb on + strand at 610.098 kb on + strand at 610.098 kb on + strand at 610.099 kb on - strand at 610.099 kb on - strand at 610.220 kb on + strand, within RR42_RS23555 at 610.221 kb on - strand, within RR42_RS23555 at 610.257 kb on - strand, within RR42_RS23555 at 610.266 kb on + strand, within RR42_RS23555 at 610.472 kb on + strand, within RR42_RS23555 at 610.472 kb on + strand, within RR42_RS23555 at 610.473 kb on - strand, within RR42_RS23555
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 607,165 - RR42_RS23535 0.86 +2.0 607,274 + -0.1 607,365 + -0.5 607,476 - RR42_RS23540 0.17 +0.0 607,900 + RR42_RS23540 0.82 -0.4 607,901 - RR42_RS23540 0.82 -0.7 607,901 - RR42_RS23540 0.82 +0.2 607,901 - RR42_RS23540 0.82 -1.6 608,081 + -0.4 608,081 + +1.3 608,082 - -1.1 608,082 - -0.4 608,082 - -0.1 608,106 - +0.5 608,819 + RR42_RS23545 0.46 +0.7 608,820 - RR42_RS23545 0.46 -0.0 608,820 - RR42_RS23545 0.46 -0.1 608,820 - RR42_RS23545 0.46 +0.6 609,075 + RR42_RS23545 0.64 +0.1 609,148 - RR42_RS23545 0.69 -1.3 609,334 + RR42_RS23545 0.82 -1.2 609,335 - RR42_RS23545 0.82 -0.6 609,585 + +4.2 609,667 + RR42_RS23550 0.10 -0.1 609,667 + RR42_RS23550 0.10 -0.1 609,667 + RR42_RS23550 0.10 -0.3 609,667 + RR42_RS23550 0.10 -0.4 609,668 - RR42_RS23550 0.10 +0.2 609,732 - RR42_RS23550 0.24 -0.1 609,732 - RR42_RS23550 0.24 +0.2 610,052 + +2.5 610,053 - -2.9 610,070 + +0.7 610,070 + +0.6 610,070 + +1.1 610,098 + -0.9 610,098 + -0.3 610,098 + -0.6 610,099 - +0.6 610,099 - +0.3 610,220 + RR42_RS23555 0.10 -0.5 610,221 - RR42_RS23555 0.10 -0.1 610,257 - RR42_RS23555 0.13 -0.5 610,266 + RR42_RS23555 0.13 -0.8 610,472 + RR42_RS23555 0.28 -0.4 610,472 + RR42_RS23555 0.28 +0.4 610,473 - RR42_RS23555 0.28 +0.4
Or see this region's nucleotide sequence