Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15545 and RR42_RS15550 are separated by 7 nucleotides RR42_RS15550 and RR42_RS15555 are separated by 60 nucleotides RR42_RS15555 and RR42_RS15560 are separated by 84 nucleotides RR42_RS15560 and RR42_RS15565 are separated by 258 nucleotides
RR42_RS15545: RR42_RS15545 - pilus assembly protein PilE, at 3,358,412 to 3,358,861
_RS15545
RR42_RS15550: RR42_RS15550 - pilus assembly protein, at 3,358,869 to 3,359,441
_RS15550
RR42_RS15555: RR42_RS15555 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 3,359,502 to 3,360,620
_RS15555
RR42_RS15560: RR42_RS15560 - riboflavin synthase subunit alpha, at 3,360,705 to 3,361,334
_RS15560
RR42_RS15565: RR42_RS15565 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,361,593 to 3,362,705
_RS15565
Position (kb)
3359
3360
3361 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3358.519 kb on + strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3358.520 kb on - strand, within RR42_RS15545 at 3359.093 kb on - strand, within RR42_RS15550 at 3359.255 kb on + strand, within RR42_RS15550 at 3359.255 kb on + strand, within RR42_RS15550 at 3359.255 kb on + strand, within RR42_RS15550 at 3359.256 kb on - strand, within RR42_RS15550 at 3359.256 kb on - strand, within RR42_RS15550 at 3359.256 kb on - strand, within RR42_RS15550 at 3359.455 kb on + strand at 3359.455 kb on + strand at 3359.455 kb on + strand at 3359.455 kb on + strand at 3360.668 kb on + strand at 3360.668 kb on + strand at 3360.669 kb on - strand at 3361.460 kb on + strand at 3361.465 kb on + strand at 3361.465 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 3,358,519 + RR42_RS15545 0.24 -0.2 3,358,520 - RR42_RS15545 0.24 -1.5 3,358,520 - RR42_RS15545 0.24 -0.3 3,358,520 - RR42_RS15545 0.24 -1.1 3,358,520 - RR42_RS15545 0.24 -0.7 3,359,093 - RR42_RS15550 0.39 +3.0 3,359,255 + RR42_RS15550 0.67 +0.4 3,359,255 + RR42_RS15550 0.67 -0.2 3,359,255 + RR42_RS15550 0.67 -0.5 3,359,256 - RR42_RS15550 0.68 +0.4 3,359,256 - RR42_RS15550 0.68 +0.3 3,359,256 - RR42_RS15550 0.68 -1.3 3,359,455 + -0.5 3,359,455 + -0.1 3,359,455 + +0.3 3,359,455 + +0.9 3,360,668 + +1.1 3,360,668 + +1.9 3,360,669 - +0.4 3,361,460 + -0.2 3,361,465 + +0.2 3,361,465 + -1.2
Or see this region's nucleotide sequence