Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00360 and RR42_RS00365 are separated by 247 nucleotides RR42_RS00365 and RR42_RS00370 are separated by 13 nucleotides RR42_RS00370 and RR42_RS00375 are separated by 95 nucleotides
RR42_RS00360: RR42_RS00360 - Asp/Glu/hydantoin racemase, at 97,832 to 98,569
_RS00360
RR42_RS00365: RR42_RS00365 - dihydropyrimidinase, at 98,817 to 100,232
_RS00365
RR42_RS00370: RR42_RS00370 - Asp/Glu/hydantoin racemase, at 100,246 to 100,974
_RS00370
RR42_RS00375: RR42_RS00375 - MFS transporter, at 101,070 to 102,368
_RS00375
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 97.834 kb on - strand at 97.834 kb on - strand at 98.440 kb on + strand, within RR42_RS00360 at 98.443 kb on - strand, within RR42_RS00360 at 98.543 kb on - strand at 98.551 kb on + strand at 98.551 kb on + strand at 98.551 kb on + strand at 98.552 kb on - strand at 98.563 kb on + strand at 98.647 kb on - strand at 98.657 kb on - strand at 98.702 kb on - strand at 98.767 kb on + strand at 98.788 kb on - strand at 98.788 kb on - strand at 98.878 kb on + strand at 98.879 kb on - strand at 98.879 kb on - strand at 98.879 kb on - strand at 98.936 kb on + strand at 98.953 kb on + strand at 98.954 kb on - strand at 98.954 kb on - strand at 98.963 kb on - strand, within RR42_RS00365 at 98.988 kb on - strand, within RR42_RS00365 at 98.988 kb on - strand, within RR42_RS00365 at 99.079 kb on + strand, within RR42_RS00365 at 99.253 kb on + strand, within RR42_RS00365 at 99.377 kb on - strand, within RR42_RS00365 at 99.377 kb on - strand, within RR42_RS00365 at 99.402 kb on - strand, within RR42_RS00365 at 99.418 kb on + strand, within RR42_RS00365 at 99.425 kb on - strand, within RR42_RS00365 at 99.741 kb on + strand, within RR42_RS00365 at 99.758 kb on - strand, within RR42_RS00365 at 99.766 kb on + strand, within RR42_RS00365 at 99.767 kb on - strand, within RR42_RS00365 at 99.767 kb on - strand, within RR42_RS00365 at 99.784 kb on + strand, within RR42_RS00365 at 99.784 kb on + strand, within RR42_RS00365 at 99.785 kb on - strand, within RR42_RS00365 at 99.862 kb on + strand, within RR42_RS00365 at 100.347 kb on - strand, within RR42_RS00370 at 100.496 kb on + strand, within RR42_RS00370 at 100.497 kb on - strand, within RR42_RS00370 at 100.497 kb on - strand, within RR42_RS00370 at 100.497 kb on - strand, within RR42_RS00370 at 100.554 kb on - strand, within RR42_RS00370 at 100.979 kb on + strand at 100.990 kb on - strand at 100.990 kb on - strand at 100.990 kb on - strand at 101.040 kb on + strand at 101.040 kb on + strand at 101.040 kb on + strand at 101.041 kb on - strand at 101.041 kb on - strand at 101.046 kb on + strand at 101.046 kb on + strand at 101.047 kb on - strand at 101.047 kb on - strand at 101.047 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 97,834 - +0.8 97,834 - -1.8 98,440 + RR42_RS00360 0.82 +1.2 98,443 - RR42_RS00360 0.83 -0.8 98,543 - +0.1 98,551 + -0.7 98,551 + +3.4 98,551 + -0.5 98,552 - -1.5 98,563 + -0.4 98,647 - -1.8 98,657 - -0.6 98,702 - -2.4 98,767 + +2.4 98,788 - +0.4 98,788 - -0.2 98,878 + +0.4 98,879 - -0.8 98,879 - +1.1 98,879 - -1.7 98,936 + -0.5 98,953 + +0.3 98,954 - +0.1 98,954 - -2.0 98,963 - RR42_RS00365 0.10 +1.7 98,988 - RR42_RS00365 0.12 +0.3 98,988 - RR42_RS00365 0.12 -2.9 99,079 + RR42_RS00365 0.19 +0.1 99,253 + RR42_RS00365 0.31 -1.1 99,377 - RR42_RS00365 0.40 +0.4 99,377 - RR42_RS00365 0.40 +0.2 99,402 - RR42_RS00365 0.41 +1.9 99,418 + RR42_RS00365 0.42 -0.1 99,425 - RR42_RS00365 0.43 -0.8 99,741 + RR42_RS00365 0.65 +0.8 99,758 - RR42_RS00365 0.66 -0.5 99,766 + RR42_RS00365 0.67 -0.1 99,767 - RR42_RS00365 0.67 +1.4 99,767 - RR42_RS00365 0.67 -0.3 99,784 + RR42_RS00365 0.68 +0.8 99,784 + RR42_RS00365 0.68 -0.1 99,785 - RR42_RS00365 0.68 +0.2 99,862 + RR42_RS00365 0.74 -0.3 100,347 - RR42_RS00370 0.14 -2.3 100,496 + RR42_RS00370 0.34 +0.8 100,497 - RR42_RS00370 0.34 -0.5 100,497 - RR42_RS00370 0.34 +0.2 100,497 - RR42_RS00370 0.34 -1.5 100,554 - RR42_RS00370 0.42 +2.3 100,979 + -0.6 100,990 - +0.5 100,990 - +0.5 100,990 - -1.0 101,040 + -0.3 101,040 + -1.8 101,040 + +0.8 101,041 - +0.7 101,041 - -0.3 101,046 + -1.3 101,046 + +0.7 101,047 - +1.7 101,047 - +0.2 101,047 - +0.3
Or see this region's nucleotide sequence