Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26205 and BWI76_RS26210 are separated by 80 nucleotides BWI76_RS26210 and BWI76_RS26215 overlap by 4 nucleotides
BWI76_RS26205: BWI76_RS26205 - glucose-1-phosphate adenylyltransferase, at 5,420,888 to 5,422,183
_RS26205
BWI76_RS26210: BWI76_RS26210 - glycogen debranching enzyme, at 5,422,264 to 5,424,240
_RS26210
BWI76_RS26215: BWI76_RS26215 - glycogen-branching enzyme, at 5,424,237 to 5,426,423
_RS26215
Position (kb) 5422
5423
5424
5425 Strain fitness (log2 ratio) -1
0
1
2 at 5421.506 kb on - strand, within BWI76_RS26205 at 5421.640 kb on + strand, within BWI76_RS26205 at 5421.648 kb on - strand, within BWI76_RS26205 at 5421.946 kb on + strand, within BWI76_RS26205 at 5422.013 kb on + strand, within BWI76_RS26205 at 5422.099 kb on - strand at 5422.263 kb on - strand at 5422.333 kb on - strand at 5422.360 kb on + strand at 5422.373 kb on + strand at 5422.381 kb on - strand at 5422.462 kb on - strand, within BWI76_RS26210 at 5422.483 kb on + strand, within BWI76_RS26210 at 5422.516 kb on + strand, within BWI76_RS26210 at 5422.864 kb on - strand, within BWI76_RS26210 at 5422.910 kb on - strand, within BWI76_RS26210 at 5422.985 kb on + strand, within BWI76_RS26210 at 5423.454 kb on - strand, within BWI76_RS26210 at 5423.763 kb on - strand, within BWI76_RS26210 at 5423.770 kb on - strand, within BWI76_RS26210 at 5423.802 kb on - strand, within BWI76_RS26210 at 5423.915 kb on + strand, within BWI76_RS26210 at 5423.978 kb on - strand, within BWI76_RS26210 at 5424.138 kb on + strand at 5424.183 kb on - strand at 5424.345 kb on - strand at 5424.504 kb on + strand, within BWI76_RS26215 at 5424.604 kb on - strand, within BWI76_RS26215 at 5424.652 kb on + strand, within BWI76_RS26215 at 5424.675 kb on + strand, within BWI76_RS26215 at 5424.786 kb on + strand, within BWI76_RS26215
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 5,421,506 - BWI76_RS26205 0.48 +0.7 5,421,640 + BWI76_RS26205 0.58 +1.4 5,421,648 - BWI76_RS26205 0.59 +1.2 5,421,946 + BWI76_RS26205 0.82 +0.4 5,422,013 + BWI76_RS26205 0.87 -1.2 5,422,099 - +1.3 5,422,263 - -1.0 5,422,333 - -1.0 5,422,360 + +0.9 5,422,373 + +2.0 5,422,381 - -0.9 5,422,462 - BWI76_RS26210 0.10 -0.1 5,422,483 + BWI76_RS26210 0.11 +1.2 5,422,516 + BWI76_RS26210 0.13 -0.5 5,422,864 - BWI76_RS26210 0.30 -1.5 5,422,910 - BWI76_RS26210 0.33 +0.9 5,422,985 + BWI76_RS26210 0.36 +0.3 5,423,454 - BWI76_RS26210 0.60 -1.6 5,423,763 - BWI76_RS26210 0.76 +0.8 5,423,770 - BWI76_RS26210 0.76 +0.8 5,423,802 - BWI76_RS26210 0.78 +0.1 5,423,915 + BWI76_RS26210 0.84 -0.3 5,423,978 - BWI76_RS26210 0.87 -0.4 5,424,138 + +0.6 5,424,183 - -0.1 5,424,345 - -1.4 5,424,504 + BWI76_RS26215 0.12 +1.1 5,424,604 - BWI76_RS26215 0.17 -1.6 5,424,652 + BWI76_RS26215 0.19 +1.1 5,424,675 + BWI76_RS26215 0.20 +1.3 5,424,786 + BWI76_RS26215 0.25 +0.2
Or see this region's nucleotide sequence