Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24225 and BWI76_RS24230 are separated by 181 nucleotides BWI76_RS24230 and BWI76_RS24235 are separated by 334 nucleotides BWI76_RS24235 and BWI76_RS24240 are separated by 117 nucleotides BWI76_RS24240 and BWI76_RS24245 overlap by 59 nucleotides
BWI76_RS24225: BWI76_RS24225 - hypothetical protein, at 5,049,806 to 5,050,144
_RS24225
BWI76_RS24230: BWI76_RS24230 - hypothetical protein, at 5,050,326 to 5,050,514
_RS24230
BWI76_RS24235: BWI76_RS24235 - Branched-chain-amino-acid transaminase (EC 2.6.1.42) (from data) , at 5,050,849 to 5,052,030
_RS24235
BWI76_RS24240: BWI76_RS24240 - hypothetical protein, at 5,052,148 to 5,052,354
_RS24240
BWI76_RS24245: BWI76_RS24245 - bifunctional glutathionylspermidine amidase/glutathionylspermidine synthase, at 5,052,296 to 5,054,167
_RS24245
Position (kb)
5050
5051
5052
5053 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5049.853 kb on - strand, within BWI76_RS24225 at 5050.011 kb on - strand, within BWI76_RS24225 at 5050.105 kb on - strand, within BWI76_RS24225 at 5050.107 kb on + strand, within BWI76_RS24225 at 5050.242 kb on + strand at 5050.630 kb on + strand at 5050.691 kb on + strand at 5050.691 kb on + strand at 5050.833 kb on + strand at 5051.030 kb on - strand, within BWI76_RS24235 at 5051.107 kb on + strand, within BWI76_RS24235 at 5051.542 kb on - strand, within BWI76_RS24235 at 5051.806 kb on - strand, within BWI76_RS24235 at 5051.860 kb on + strand, within BWI76_RS24235 at 5051.869 kb on + strand, within BWI76_RS24235 at 5051.869 kb on - strand, within BWI76_RS24235 at 5051.892 kb on - strand, within BWI76_RS24235 at 5051.947 kb on + strand at 5051.959 kb on - strand at 5051.970 kb on - strand at 5051.987 kb on + strand at 5052.194 kb on + strand, within BWI76_RS24240 at 5052.414 kb on + strand at 5052.453 kb on + strand at 5052.818 kb on - strand, within BWI76_RS24245 at 5052.977 kb on - strand, within BWI76_RS24245 at 5053.002 kb on + strand, within BWI76_RS24245 at 5053.021 kb on + strand, within BWI76_RS24245
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 5,049,853 - BWI76_RS24225 0.14 -0.2 5,050,011 - BWI76_RS24225 0.60 +0.6 5,050,105 - BWI76_RS24225 0.88 +0.3 5,050,107 + BWI76_RS24225 0.89 -2.5 5,050,242 + +2.6 5,050,630 + -1.5 5,050,691 + +0.7 5,050,691 + -0.4 5,050,833 + -0.1 5,051,030 - BWI76_RS24235 0.15 -0.2 5,051,107 + BWI76_RS24235 0.22 -0.7 5,051,542 - BWI76_RS24235 0.59 +0.2 5,051,806 - BWI76_RS24235 0.81 -0.7 5,051,860 + BWI76_RS24235 0.86 +0.5 5,051,869 + BWI76_RS24235 0.86 +0.4 5,051,869 - BWI76_RS24235 0.86 -0.6 5,051,892 - BWI76_RS24235 0.88 -0.6 5,051,947 + -0.3 5,051,959 - +0.0 5,051,970 - -0.3 5,051,987 + -0.7 5,052,194 + BWI76_RS24240 0.22 -0.5 5,052,414 + +1.2 5,052,453 + +0.2 5,052,818 - BWI76_RS24245 0.28 +0.7 5,052,977 - BWI76_RS24245 0.36 +1.0 5,053,002 + BWI76_RS24245 0.38 +0.5 5,053,021 + BWI76_RS24245 0.39 -0.2
Or see this region's nucleotide sequence