Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotides BWI76_RS13130 and BWI76_RS13135 overlap by 1 nucleotides BWI76_RS13135 and BWI76_RS13140 are separated by 28 nucleotides
BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349
_RS13125
BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761
_RS13130
BWI76_RS13135: BWI76_RS13135 - 3-oxoadipyl-CoA thiolase, at 2,738,761 to 2,739,966
_RS13135
BWI76_RS13140: BWI76_RS13140 - phenylacetate-CoA ligase, at 2,739,995 to 2,741,311
_RS13140
Position (kb)
2738
2739
2740 Strain fitness (log2 ratio)
-1
0
1 at 2737.786 kb on + strand, within BWI76_RS13125 at 2737.794 kb on - strand, within BWI76_RS13125 at 2737.877 kb on + strand, within BWI76_RS13125 at 2738.546 kb on + strand, within BWI76_RS13130 at 2738.845 kb on - strand at 2738.935 kb on - strand, within BWI76_RS13135 at 2739.311 kb on - strand at 2739.452 kb on + strand, within BWI76_RS13135 at 2739.460 kb on - strand, within BWI76_RS13135 at 2739.928 kb on - strand at 2739.931 kb on + strand at 2739.931 kb on + strand at 2740.433 kb on + strand, within BWI76_RS13140 at 2740.799 kb on + strand, within BWI76_RS13140 at 2740.865 kb on + strand, within BWI76_RS13140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 2,737,786 + BWI76_RS13125 0.60 -1.0 2,737,794 - BWI76_RS13125 0.61 -1.1 2,737,877 + BWI76_RS13125 0.67 -1.6 2,738,546 + BWI76_RS13130 0.49 -1.2 2,738,845 - -0.5 2,738,935 - BWI76_RS13135 0.14 +1.6 2,739,311 - +0.2 2,739,452 + BWI76_RS13135 0.57 -0.3 2,739,460 - BWI76_RS13135 0.58 -0.3 2,739,928 - -0.7 2,739,931 + +0.5 2,739,931 + -0.4 2,740,433 + BWI76_RS13140 0.33 -0.2 2,740,799 + BWI76_RS13140 0.61 -0.8 2,740,865 + BWI76_RS13140 0.66 +0.9
Or see this region's nucleotide sequence