Experiment: minimal media, aerobic, 0G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4494.1 and SO4494 overlap by 31 nucleotides SO4494 and Sp5SF are separated by 267 nucleotides Sp5SF and tRNA-Thr-3 are separated by 31 nucleotides tRNA-Thr-3 and SO_r028 are separated by 28 nucleotides SO_r028 and Sp23SF are separated by 203 nucleotides
SO4494.1: SO4494.1 - hypothetical protein (NCBI ptt file), at 4,678,390 to 4,678,620
SO4494.1
SO4494: SO4494 - hypothetical protein (NCBI ptt file), at 4,678,590 to 4,678,814
SO4494
SO_r019: Sp5SF - 5S ribosomal RNA (RefSeq), at 4,679,082 to 4,679,201
Sp5SF
SO_t101: tRNA-Thr-3 - tRNA-Thr (RefSeq), at 4,679,233 to 4,679,308
tRNA-Thr-3
SO_r028: SO_r028 - 5S ribosomal RNA (RefSeq), at 4,679,337 to 4,679,456
_r028
SO_r020: Sp23SF - 23S ribosomal RNA (RefSeq), at 4,679,660 to 4,682,552
Sp23SF
Position (kb)
4679
4680 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4678.388 kb on + strand at 4678.524 kb on + strand, within SO4494.1 at 4678.524 kb on + strand, within SO4494.1 at 4678.532 kb on - strand, within SO4494.1 at 4678.955 kb on + strand at 4678.968 kb on + strand at 4679.220 kb on + strand at 4679.228 kb on - strand at 4679.228 kb on - strand at 4679.228 kb on - strand at 4679.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration remove 4,678,388 + +2.7 4,678,524 + SO4494.1 0.58 -0.7 4,678,524 + SO4494.1 0.58 -1.9 4,678,532 - SO4494.1 0.61 +0.1 4,678,955 + +0.6 4,678,968 + -0.4 4,679,220 + +0.6 4,679,228 - -1.5 4,679,228 - -0.8 4,679,228 - +0.1 4,679,337 - -0.4
Or see this region's nucleotide sequence