Experiment: minimal media, aerobic, 0G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Ala-3 and SO_3130 are separated by 104 nucleotides SO_3130 and SO3128.2 are separated by 18 nucleotides SO3128.2 and SO3131 are separated by 261 nucleotides SO3131 and tRNA-Val-5 are separated by 173 nucleotides tRNA-Val-5 and tRNA-Val-4 are separated by 48 nucleotides tRNA-Val-4 and tRNA-Val-3 are separated by 27 nucleotides tRNA-Val-3 and tRNA-Val-2 are separated by 45 nucleotides tRNA-Val-2 and tRNA-Val-1 are separated by 26 nucleotides
SO_t072: tRNA-Ala-3 - tRNA-Ala (RefSeq), at 3,259,601 to 3,259,676
tRNA-Ala-3
SO_3130: SO_3130 - no description, at 3,259,781 to 3,260,620
_3130
SO3128.2: SO3128.2 - hypothetical glutamate tRNA synthetase (NCBI ptt file), at 3,260,639 to 3,261,190
SO3128.2
SO3131: SO3131 - hypothetical protein (NCBI ptt file), at 3,261,452 to 3,261,544
SO3131
SO_t073: tRNA-Val-5 - tRNA-Val (RefSeq), at 3,261,718 to 3,261,793
tRNA-Val-5
SO_t074: tRNA-Val-4 - tRNA-Val (RefSeq), at 3,261,842 to 3,261,917
tRNA-Val-4
SO_t075: tRNA-Val-3 - tRNA-Val (RefSeq), at 3,261,945 to 3,262,020
tRNA-Val-3
SO_t076: tRNA-Val-2 - tRNA-Val (RefSeq), at 3,262,066 to 3,262,141
tRNA-Val-2
SO_t077: tRNA-Val-1 - tRNA-Val (RefSeq), at 3,262,168 to 3,262,243
tRNA-Val-1
Position (kb)
3260
3261
3262 Strain fitness (log2 ratio)
-1
0
1 at 3261.310 kb on + strand at 3261.318 kb on - strand at 3261.443 kb on - strand at 3261.509 kb on + strand, within SO3131 at 3261.517 kb on - strand, within SO3131 at 3261.517 kb on - strand, within SO3131 at 3261.524 kb on - strand, within SO3131 at 3261.596 kb on - strand at 3261.624 kb on - strand at 3262.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration remove 3,261,310 + -0.4 3,261,318 - -1.0 3,261,443 - +0.9 3,261,509 + SO3131 0.61 -0.1 3,261,517 - SO3131 0.70 +0.3 3,261,517 - SO3131 0.70 -0.4 3,261,524 - SO3131 0.77 +0.5 3,261,596 - -0.7 3,261,624 - +0.0 3,262,143 + +1.3
Or see this region's nucleotide sequence