Experiment: minimal media, aerobic, 0G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0208 and Sp16SB are separated by 682 nucleotides Sp16SB and tRNA-Ile-1 are separated by 75 nucleotides tRNA-Ile-1 and tRNA-Ala-1 are separated by 138 nucleotides tRNA-Ala-1 and Sp23SB are separated by 274 nucleotides
SO0208: SO0208 - RNA-binding protein (NCBI ptt file), at 215,641 to 216,105
SO0208
SO_r004: Sp16SB - 16S ribosomal RNA (RefSeq), at 216,788 to 218,316
Sp16SB
SO_t003: tRNA-Ile-1 - tRNA-Ile (RefSeq), at 218,392 to 218,468
tRNA-Ile-1
SO_t004: tRNA-Ala-1 - tRNA-Ala (RefSeq), at 218,607 to 218,682
tRNA-Ala-1
SO_r005: Sp23SB - 23S ribosomal RNA (RefSeq), at 218,957 to 221,849
Sp23SB
Position (kb) 216
217
218
219 Strain fitness (log2 ratio) -1
0
1
2 at 215.790 kb on - strand, within SO0208 at 215.839 kb on - strand, within SO0208 at 215.885 kb on - strand, within SO0208 at 215.893 kb on - strand, within SO0208 at 215.927 kb on + strand, within SO0208 at 215.950 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.026 kb on + strand, within SO0208 at 216.041 kb on + strand, within SO0208 at 216.049 kb on - strand, within SO0208 at 216.243 kb on + strand at 216.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration remove 215,790 - SO0208 0.32 -0.4 215,839 - SO0208 0.43 +0.8 215,885 - SO0208 0.52 +2.7 215,893 - SO0208 0.54 +0.0 215,927 + SO0208 0.62 +0.3 215,950 + SO0208 0.66 -0.0 216,026 + SO0208 0.83 +1.1 216,026 + SO0208 0.83 -0.9 216,026 + SO0208 0.83 -0.2 216,026 + SO0208 0.83 +0.6 216,041 + SO0208 0.86 +0.6 216,049 - SO0208 0.88 -0.4 216,243 + -1.2 216,386 + +0.1
Or see this region's nucleotide sequence