Strain Fitness in Shewanella oneidensis MR-1 around SO2513

Experiment: minimal media, aerobic, 0G configuration

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2511 and SO2512 are separated by 54 nucleotidesSO2512 and SO2513 overlap by 8 nucleotidesSO2513 and nth are separated by 73 nucleotidesnth and SO2515 are separated by 29 nucleotides SO2511: SO2511 - membrane protein, putative (NCBI ptt file), at 2,639,225 to 2,640,274 SO2511 SO2512: SO2512 - conserved hypothetical protein (NCBI ptt file), at 2,640,329 to 2,640,967 SO2512 SO2513: SO2513 - NqrDE/RnfAE family protein (NCBI ptt file), at 2,640,960 to 2,641,658 SO2513 SO2514: nth - endonuclease III (NCBI ptt file), at 2,641,732 to 2,642,427 nth SO2515: SO2515 - hypothetical protein (NCBI ptt file), at 2,642,457 to 2,642,729 SO2515 Position (kb) 2640 2641 2642Strain fitness (log2 ratio) -1 0 1at 2641.907 kb on + strand, within nthat 2642.007 kb on - strand, within nthat 2642.146 kb on + strand, within nthat 2642.259 kb on - strand, within nthat 2642.272 kb on - strand, within nthat 2642.366 kb on + strandat 2642.366 kb on + strandat 2642.439 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration
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2,641,907 + nth SO2514 0.25 +0.9
2,642,007 - nth SO2514 0.40 +0.7
2,642,146 + nth SO2514 0.59 +0.1
2,642,259 - nth SO2514 0.76 +0.7
2,642,272 - nth SO2514 0.78 -0.6
2,642,366 + +0.2
2,642,366 + -0.5
2,642,439 - +0.3

Or see this region's nucleotide sequence