Experiment: minimal media, aerobic, 0G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and thiS are separated by 1 nucleotides thiS and thiF overlap by 4 nucleotides thiF and thiDE overlap by 4 nucleotides
SO2440: thiH - thiH protein (NCBI ptt file), at 2,553,968 to 2,555,095
thiH
SO2441: thiG - thiG protein (NCBI ptt file), at 2,555,092 to 2,555,856
thiG
SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061
thiS
SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960
thiF
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
Position (kb)
2556
2557 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2555.122 kb on + strand at 2555.122 kb on + strand at 2555.147 kb on + strand at 2555.156 kb on - strand at 2555.243 kb on + strand, within thiG at 2555.243 kb on + strand, within thiG at 2555.251 kb on - strand, within thiG at 2555.410 kb on + strand, within thiG at 2555.433 kb on - strand, within thiG at 2555.489 kb on - strand, within thiG at 2555.522 kb on - strand, within thiG at 2555.563 kb on + strand, within thiG at 2555.580 kb on + strand, within thiG at 2555.855 kb on + strand at 2555.919 kb on - strand, within thiS at 2556.483 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.918 kb on - strand at 2556.971 kb on - strand at 2557.138 kb on - strand, within thiDE at 2557.138 kb on - strand, within thiDE at 2557.214 kb on - strand, within thiDE at 2557.280 kb on + strand, within thiDE at 2557.413 kb on - strand, within thiDE at 2557.483 kb on + strand, within thiDE at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration remove 2,555,122 + +0.7 2,555,122 + -0.7 2,555,147 + -0.6 2,555,156 - -0.6 2,555,243 + thiG SO2441 0.20 +0.9 2,555,243 + thiG SO2441 0.20 -0.5 2,555,251 - thiG SO2441 0.21 +0.7 2,555,410 + thiG SO2441 0.42 -0.0 2,555,433 - thiG SO2441 0.45 -1.0 2,555,489 - thiG SO2441 0.52 -0.7 2,555,522 - thiG SO2441 0.56 -0.3 2,555,563 + thiG SO2441 0.62 +0.0 2,555,580 + thiG SO2441 0.64 -1.9 2,555,855 + -0.8 2,555,919 - thiS SO2442 0.30 +0.1 2,556,483 + thiF SO2443 0.47 -0.4 2,556,680 + thiF SO2443 0.69 -1.1 2,556,680 + thiF SO2443 0.69 -1.3 2,556,680 + thiF SO2443 0.69 -0.4 2,556,738 + thiF SO2443 0.75 +0.1 2,556,738 + thiF SO2443 0.75 -1.3 2,556,918 - -0.2 2,556,971 - +0.4 2,557,138 - thiDE SO2444 0.11 -2.1 2,557,138 - thiDE SO2444 0.11 -0.8 2,557,214 - thiDE SO2444 0.16 -0.7 2,557,280 + thiDE SO2444 0.20 -3.4 2,557,413 - thiDE SO2444 0.29 +0.4 2,557,483 + thiDE SO2444 0.33 -1.5 2,557,552 + thiDE SO2444 0.38 -1.6 2,557,555 - thiDE SO2444 0.38 +0.7 2,557,676 + thiDE SO2444 0.45 +0.2 2,557,690 + thiDE SO2444 0.46 -0.2 2,557,723 + thiDE SO2444 0.48 -0.3 2,557,729 + thiDE SO2444 0.49 -1.1 2,557,742 + thiDE SO2444 0.50 -0.3 2,557,760 - thiDE SO2444 0.51 -0.3 2,557,770 + thiDE SO2444 0.51 -0.9 2,557,778 - thiDE SO2444 0.52 -0.6 2,557,782 + thiDE SO2444 0.52 -0.3 2,557,790 - thiDE SO2444 0.53 -0.6 2,557,794 + thiDE SO2444 0.53 +0.4 2,557,874 + thiDE SO2444 0.58 -1.6 2,557,874 + thiDE SO2444 0.58 -0.1
Or see this region's nucleotide sequence