Experiment: minimal media, aerobic, 0G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gsk and nadE are separated by 224 nucleotides nadE and SO2022 are separated by 60 nucleotides SO2022 and SO2024 are separated by 409 nucleotides
SO2020: gsk - inosine-guanosine kinase (NCBI ptt file), at 2,119,424 to 2,120,728
gsk
SO2021: nadE - NH(3)-dependent NAD(+) synthetase (NCBI ptt file), at 2,120,953 to 2,121,783
nadE
SO2022: SO2022 - hypothetical protein (NCBI ptt file), at 2,121,844 to 2,122,368
SO2022
SO2024: SO2024 - hypothetical protein (NCBI ptt file), at 2,122,778 to 2,124,004
SO2024
Position (kb)
2120
2121
2122 Strain fitness (log2 ratio)
-1
0
1 at 2119.986 kb on - strand, within gsk at 2120.025 kb on + strand, within gsk at 2120.197 kb on - strand, within gsk at 2120.344 kb on - strand, within gsk at 2120.547 kb on + strand, within gsk at 2120.547 kb on + strand, within gsk at 2120.593 kb on - strand, within gsk at 2120.659 kb on - strand at 2120.678 kb on + strand at 2120.708 kb on - strand at 2120.708 kb on - strand at 2120.810 kb on + strand at 2120.894 kb on - strand at 2121.920 kb on + strand, within SO2022 at 2121.920 kb on + strand, within SO2022 at 2121.922 kb on - strand, within SO2022 at 2121.948 kb on - strand, within SO2022 at 2121.963 kb on - strand, within SO2022 at 2122.043 kb on + strand, within SO2022 at 2122.043 kb on + strand, within SO2022 at 2122.067 kb on + strand, within SO2022 at 2122.075 kb on + strand, within SO2022 at 2122.311 kb on + strand, within SO2022 at 2122.371 kb on - strand at 2122.389 kb on - strand at 2122.414 kb on - strand at 2122.420 kb on - strand at 2122.464 kb on - strand at 2122.517 kb on + strand at 2122.517 kb on + strand at 2122.517 kb on + strand at 2122.525 kb on - strand at 2122.556 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration remove 2,119,986 - gsk SO2020 0.43 +0.7 2,120,025 + gsk SO2020 0.46 +0.3 2,120,197 - gsk SO2020 0.59 +0.8 2,120,344 - gsk SO2020 0.70 -0.2 2,120,547 + gsk SO2020 0.86 +0.4 2,120,547 + gsk SO2020 0.86 -0.2 2,120,593 - gsk SO2020 0.90 +0.1 2,120,659 - -0.8 2,120,678 + +1.1 2,120,708 - -0.5 2,120,708 - -0.5 2,120,810 + -0.1 2,120,894 - -0.2 2,121,920 + SO2022 0.14 +1.4 2,121,920 + SO2022 0.14 -0.4 2,121,922 - SO2022 0.15 +1.2 2,121,948 - SO2022 0.20 +0.1 2,121,963 - SO2022 0.23 +1.1 2,122,043 + SO2022 0.38 +0.1 2,122,043 + SO2022 0.38 +0.3 2,122,067 + SO2022 0.42 +1.0 2,122,075 + SO2022 0.44 -1.2 2,122,311 + SO2022 0.89 -1.2 2,122,371 - +1.1 2,122,389 - +0.0 2,122,414 - +0.1 2,122,420 - +0.7 2,122,464 - -0.8 2,122,517 + +0.0 2,122,517 + -0.5 2,122,517 + +0.2 2,122,525 - -0.2 2,122,556 - -0.6
Or see this region's nucleotide sequence