Experiment: minimal media, aerobic, 0G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsP and SO1333 are separated by 92 nucleotides SO1333 and lgt are separated by 45 nucleotides lgt and thyA overlap by 1 nucleotides thyA and nhaA are separated by 118 nucleotides
SO1332: ptsP - phosphoenolpyruvate-protein phosphotransferase PtsP (NCBI ptt file), at 1,389,120 to 1,391,354
ptsP
SO1333: SO1333 - conserved hypothetical protein (NCBI ptt file), at 1,391,447 to 1,392,214
SO1333
SO1334: lgt - prolipoprotein diacylglyceryl transferase (NCBI ptt file), at 1,392,260 to 1,393,066
lgt
SO1335: thyA - thymidylate synthase (NCBI ptt file), at 1,393,066 to 1,393,860
thyA
SO1336: nhaA - Na+/H+ antiporter (NCBI ptt file), at 1,393,979 to 1,395,148
nhaA
Position (kb)
1392
1393
1394 Strain fitness (log2 ratio)
-2
-1
0
1 at 1391.267 kb on + strand at 1391.267 kb on + strand at 1391.275 kb on - strand at 1391.295 kb on - strand at 1391.295 kb on - strand at 1391.370 kb on + strand at 1391.402 kb on + strand at 1391.480 kb on + strand at 1391.480 kb on + strand at 1391.514 kb on + strand at 1391.689 kb on + strand, within SO1333 at 1391.808 kb on - strand, within SO1333 at 1392.040 kb on + strand, within SO1333 at 1394.042 kb on + strand at 1394.048 kb on + strand at 1394.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction minimal media, aerobic, 0G configuration remove 1,391,267 + +0.6 1,391,267 + -0.1 1,391,275 - -0.4 1,391,295 - -0.6 1,391,295 - +0.3 1,391,370 + -0.4 1,391,402 + -0.8 1,391,480 + -1.9 1,391,480 + -0.1 1,391,514 + -1.8 1,391,689 + SO1333 0.32 -2.3 1,391,808 - SO1333 0.47 -0.1 1,392,040 + SO1333 0.77 -1.6 1,394,042 + -0.3 1,394,048 + -0.3 1,394,065 + +0.2
Or see this region's nucleotide sequence